Invitrogen

PDGFRB Monoclonal Antibody (G.290.3)

Advanced Verification

IP-mass spectrometry

4 Published Figures

12 References

View all (90) PDGFRB (CD140b) antibodies

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PDGFRB Antibody (MA5-15143)

IP-MS enrichment of PDGFRB (LFQ intensity): PDGFRB was enriched 83-fold from NIH/3T3 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and PDGFRB antibody (Product # MA5-15143). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of... View More

PDGFRB Antibody in Immunocytochemistry (ICC/IF)

PDGFRB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PDGFRB Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

PDGFRB Antibody in Immunohistochemistry (IHC)

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Product Details

MA5-15143

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:100-1:400

View 3 publications 3 publications

Immunoprecipitation (IP)

1:50

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

G.290.3

Immunogen

GST fusion protein containing a carboxy-terminal fragment of human PDGF receptor ß

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

4 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10985851

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

PDGFRB is a receptor tyrosine kinase and has been implicated in atherogenesis and in cell transformation by the SIS oncogene. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. The gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses PDGFRB (CD140b) to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

High Resolution Multiplex Confocal Imaging of the Neurovascular Unit in Health and Experimental Ischemic Stroke.

Cells

Lochhead JJ,Williams EI,Reddell ES,Dorn E,Ronaldson PT,Davis TP

MA5-15143 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to demonstrate ischemia-induced astrocyte activation, neurite loss, and microglial migration toward blood vessels in a single confocal image.

Fri Feb 17 00:00:00 EST 2023

Activating NO-sGC crosstalk in the mouse vascular niche promotes vascular integrity and mitigates acute lung injury.

The Journal of experimental medicine

He H,Yang W,Su N,Zhang C,Dai J,Han F,Singhal M,Bai W,Zhu X,Zhu J,Liu Z,Xia W,Liu X,Zhang C,Jiang K,Huang W,Chen D,Wang Z,He X,Kirchhoff F,Li Z,Liu C,Huan J,Wang X,Wei W,Wang J,Augustin HG,Hu J

MA5-15143 was used in Immunohistochemistry to demonstrate that impaired NO-sGC crosstalk in the vascular niche results in elevated vascular permeability, and pharmacological activation of this crosstalk represents a promising translational therapy for ALI.

Mon Feb 06 00:00:00 EST 2023

Improving hindlimb locomotor function by Non-invasive AAV-mediated manipulations of propriospinal neurons in mice with complete spinal cord injury.

Nature communications

Brommer B,He M,Zhang Z,Yang Z,Page JC,Su J,Zhang Y,Zhu J,Gouy E,Tang J,Williams P,Dai W,Wang Q,Solinsky R,Chen B,He Z

MA5-15143 was used in Immunohistochemistry to demonstrate a strategy of engaging thoracic propriospinal neurons to improve hindlimb function and provide insights into optimizing neuromodulation-based strategies for treating SCI.

Wed Feb 03 00:00:00 EST 2021

Epigenetic Reprogramming of Cancer-Associated Fibroblasts Deregulates Glucose Metabolism and Facilitates Progression of Breast Cancer.

Cell reports

Becker LM,O'Connell JT,Vo AP,Cain MP,Tampe D,Bizarro L,Sugimoto H,McGow AK,Asara JM,Lovisa S,McAndrews KM,Zielinski R,Lorenzi PL,Zeisberg M,Raza S,LeBleu VS,Kalluri R

MA5-15143 was used in Immunohistochemistry-immunofluorescence to demonstrate that cancer-associated fibroblasts exhibit a metabolic shift toward lactate and pyruvate production and fuel biosynthetic pathways of cancer cells.

Tue Jun 02 00:00:00 EDT 2020

PDGF Modulates BMP2-Induced Osteogenesis in Periosteal Progenitor Cells.

JBMR plus

Wang X,Matthews BG,Yu J,Novak S,Grcevic D,Sanjay A,Kalajzic I

MA5-15143 was used in Immunohistochemistry to show BMP2-induced osteogenesis imperfecta in periosteal progenitor cells can be inhibited by platelet-derived growth factor which decreases stimulation of the canonical BMP2/Smad pathway and downstream target gene expression.

Wed May 01 00:00:00 EDT 2019

PDGF Modulates BMP2-Induced Osteogenesis in Periosteal Progenitor Cells.

JBMR plus

Wang X,Matthews BG,Yu J,Novak S,Grcevic D,Sanjay A,Kalajzic I

Wed May 01 00:00:00 EDT 2019

Zoledronate suppressed angiogenesis and osteogenesis by inhibiting osteoclasts formation and secretion of PDGF-BB.

PloS one

Gao SY,Zheng GS,Wang L,Liang YJ,Zhang SE,Lao XM,Li K,Liao GQ

Published figure using PDGFRB monoclonal antibody (Product # MA5-15143) in Western Blot

Mon Sep 18 00:00:00 EDT 2017

Dynamics of PDGFRβ expression in different cell types after brain injury.

Glia

Kyyriäinen J,Ekolle Ndode-Ekane X,Pitkänen A

MA5-15143 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to reveal an acute accumulation of PDGFRβ+ BBB-related cells in degenerating brain areas, which can be long lasting, suggesting an active role for PDGFRβ-signaling in blood vessel and post-injury tissue recovery.

Wed Feb 01 00:00:00 EST 2017

Dynamics of PDGFRβ expression in different cell types after brain injury.

Glia

Kyyriäinen J,Ekolle Ndode-Ekane X,Pitkänen A

Wed Feb 01 00:00:00 EST 2017

Osteogenic potential of alpha smooth muscle actin expressing muscle resident progenitor cells.

Bone

Matthews BG,Torreggiani E,Roeder E,Matic I,Grcevic D,Kalajzic I

MA5-15143 was used in immunohistochemistry to characterize alpha smooth muscle actin for osteogenic potential by expression muscle resident progenitor cells

Tue Mar 01 00:00:00 EST 2016

Heterogeneous perivascular cell coverage affects breast cancer metastasis and response to chemotherapy.

JCI insight

Kim J,de Sampaio PC,Lundy DM,Peng Q,Evans KW,Sugimoto H,Gagea M,Kienast Y,Amaral NSD,Rocha RM,Eikesdal HP,Lønning PE,Meric-Bernstam F,LeBleu VS

Published figure using PDGFRB monoclonal antibody (Product # MA5-15143) in Immunofluorescence

Thu Dec 22 00:00:00 EST 2016

Heterogeneous perivascular cell coverage affects breast cancer metastasis and response to chemotherapy.

JCI insight

Kim J,de Sampaio PC,Lundy DM,Peng Q,Evans KW,Sugimoto H,Gagea M,Kienast Y,Amaral NSD,Rocha RM,Eikesdal HP,Lønning PE,Meric-Bernstam F,LeBleu VS

Published figure using PDGFRB monoclonal antibody (Product # MA5-15143) in Immunofluorescence

Thu Dec 22 00:00:00 EST 2016

Bile acids induce hepatic differentiation of mesenchymal stem cells.

Scientific reports

Sawitza I,Kordes C,Götze S,Herebian D,Häussinger D

MA5-15143 was used in immunocytochemistry to study the effect of bile acids on mesenchymal stem cells in liver

Tue Aug 25 00:00:00 EDT 2015

Hepatic stellate cells support hematopoiesis and are liver-resident mesenchymal stem cells.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Kordes C,Sawitza I,Götze S,Häussinger D

MA5-15143 was used in immunocytochemistry to study the role of hepatic stellate cells as liver-resident mesenchymal stem cells and their role in hematopoiesis

Mon Sep 16 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b; CD140b antigen; PDGF beta chain; PDGF Receptor beta; PDGF-R-beta; PDGFR-1; PDGFR-beta; Platelet-derived growth factor receptor 1; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor beta variant 1; Platelet-derived growth factor receptor, beta; platelet-derived growth factor receptor, beta polypeptide

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Gene Aliases: AI528809; CD140B; IBGC4; IMF1; JTK12; KOGS; PDGFR; PDGFR-1; PDGFR1; PDGFRB; PENTT

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UniProt ID: (Human) P09619, (Rat) Q05030, (Mouse) P05622

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Entrez Gene ID: (Human) 5159, (Rat) 24629, (Mouse) 18596

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Function(s)

protein tyrosine kinase activity platelet activating factor receptor activity platelet-derived growth factor-activated receptor activity platelet-derived growth factor beta-receptor activity Ras guanyl-nucleotide exchange factor activity receptor binding platelet-derived growth factor receptor binding protein binding ATP binding protein kinase binding vascular endothelial growth factor binding phosphatidylinositol 3-kinase binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity platelet-derived growth factor binding protein kinase activity signal transducer activity kinase activity nucleotide binding transmembrane receptor protein tyrosine kinase activity transferase activity transferase activity, transferring phosphorus-containing groups transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PDGFRB Monoclonal Antibody (G.290.3)

PDGFRB Antibody (MA5-15143)

Product Details

MA5-15143

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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