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Invitrogen
PDI Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
PDI Antibody (PA5-77821) in WB
Western blot was performed using Anti-PDI Polyclonal Antibody (Product # PA5-77821) and a 57 kDa band corresponding to PDI was observed across the cell lines and tissues tested. Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane2), NIH/3T3 (Lane 3), A-375 (Lane 4), A-431 (Lane 5), HEK-293 (Lane 6), and tissue extracts of Mouse Liver (Lane 7), Rat Liver (lane 8) and Rat Testis (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nit... View More
Product Details
PA5-77821
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Guinea pig,
Hamster,
Human,
Mollusc,
Mouse,
Sheep,
Pig,
Rat,
Xenopus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Rat PDI synthetic peptide (409-509 aa C-terminal) conjugated to KLH
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2736334
Product Specific Information
A 1:1,000 dilution of PA5-77821 was sufficient for detection of PDI in 20 µg of HeLa cell lysate by ECL immunoblot analysis.|Detects approximately 58kDa.
Target Information
Protein disulfide isomerase (PDI) is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, the protein seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. It may therefore cause structural modifications of exofacial proteins. Inside the cell, PDI seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, it functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). PDI may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CaO19.12595; cellular thyroid hormone binding protein; Cellular thyroid hormone-binding protein; collagen prolyl 4-hydroxylase beta; Endoplasmic reticulum resident protein 59; Eps1p; ER protein 59; glutathione-insulin transhydrogenase; p55; p58; pancreas-specific protein disulfide isomerase; PDI; PDI (E.C.5.3.4.1); PDI1P; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; prolyl 4-hydroxylase beta polypeptide; Prolyl 4-hydroxylase subunit beta; prolyl 4-hydroxylase, beta polypeptide; prolyl 4-hydroxylase, beta subunit; protein disulfide isomerase; Protein disulfide isomerase (Prolyl 4-hydroxylase, beta polypeptide); protein disulfide isomerase A2; protein disulfide isomerase family A, member 1; protein disulfide isomerase family A, member 2; protein disulfide isomerase-associated 1; protein disulfide isomerase/oxidoreductase; Protein disulfide-isomerase; protocollagen hydroxylase; testicular secretory protein Li 32; thyroid hormone-binding protein p55
Gene Aliases: CLCRP1; DSI; ERBA2L; ERp59; GIT; I79_002399; P4HB; P4Hbeta; p58; PDI; PDIA1; pdia2; pdip; PDIR; PHDB; PO4DB; PO4HB; PROHB; Thbp; XELAEV_18043753mg; XELAEV_18045582mg; XPDIp
UniProt ID: (Human) P07237, (Bovine) P05307, (Rat) P04785, (Mouse) P09103, (Chinese hamster) Q8R4U2
response to reactive oxygen species
protein folding
peptidyl-proline hydroxylation to 4-hydroxy-L-proline
response to endoplasmic reticulum stress
lipoprotein biosynthetic process
cell redox homeostasis
positive regulation of viral entry into host cell
cellular response to hypoxia
regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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