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PDK1 Polyclonal Antibody

PDK1 Antibody in Immunocytochemistry (ICC/IF)

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PDK1 Antibody (18262-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol) fixed HEK-293 cells using 18262-1-AP (PDK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PDK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

18262-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PDK1 Fusion Protein Ag13121 (1-436 aa encoded by BC039158)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is specific to PDK1.

Immunogen sequence: MRLARLLRG AALAGPGPGL RAAGFSRSFS SDSGSSPASE RGVPGQVDFY ARFSPSPLSM KQFLDFGSVN ACEKTSFMFL RQELPVRLAN IMKEISLLPD NLLRTPSVQL VQSWYIQSLQ ELLDFKDKSA EDAKAIYDFT DTVIRIRNRH NDVIPTMAQG VIEYKESFGV DPVTSQNVQY FLDRFYMSRI SIRMLLNQHS LLFGGKGKGS PSHRKHIGSI NPNCNVLEVI KDGYENARRL CDLYYINSPE LELEELNAKS PGQPIQVVYV PSHLYHMVFE LFKNAMRATM EHHANRGVYP PIQVHVTLGN EDLTVKMSDR GGGVPLRKID RLFNYMYSTA PRPRVETSRA VPLAGFGYGL PISRLYAQYF QGDLKLYSLE GYGTDAVIYI KALSTDSIER LPVYNKAAWK HYNTNHEADD WCVPSREPKD MTTFRSA (1-436 aa encoded by BC039158)

Target Information

PDK1 (3-phosphoinositide dependent kinase-1) is a serine/threonine protein kinase localized to the plasma membrane. It plays a vital role in insulin-induced glycogen synthesis, PKC activation, and activation of the AGC subfamily of protein kinases. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PDK1-stabilized LncRNA SPRY4-IT1 promotes breast cancer progression via activating NF-κB signaling pathway.

Molecular carcinogenesis

Zhang Y,Chen H,Yuan R,Wang Y,Zhang D,Zeng Q,Jiang W,Zhang R,Chen T,Chai C,Guo B

Sat Jul 01 00:00:00 EDT 2023

Inhibition of phosphoglycerate kinase 1 attenuates autoimmune myocarditis by reprogramming CD4+ T cell metabolism.

Cardiovascular research

Lu Y,Zhao N,Wu Y,Yang S,Wu Q,Dong Q,Du Y

Tue Jun 13 00:00:00 EDT 2023

Inhibition of phosphoglycerate kinase 1 attenuates autoimmune myocarditis by reprogramming CD4+ T cell metabolism.

Cardiovascular research

Lu Y,Zhao N,Wu Y,Yang S,Wu Q,Dong Q,Du Y

Tue Jun 13 00:00:00 EDT 2023

A novel polypeptide encoded by the circular RNA ZKSCAN1 suppresses HCC via degradation of mTOR.

Molecular cancer

Song R,Ma S,Xu J,Ren X,Guo P,Liu H,Li P,Yin F,Liu M,Wang Q,Yu L,Liu J,Duan B,Rahman NA,Wołczyński S,Li G,Li X

Mon Jan 23 00:00:00 EST 2023

PDK1 regulates the progression of esophageal squamous cell carcinoma through metabolic reprogramming.

Molecular carcinogenesis

Ma Y,Zhang X,Han X,Hao Z,Ji R,Li J,Guo W,Zhang Y,Guan F,Ma S

Thu Jun 01 00:00:00 EDT 2023

PDK1 regulates the progression of esophageal squamous cell carcinoma through metabolic reprogramming.

Molecular carcinogenesis

Ma Y,Zhang X,Han X,Hao Z,Ji R,Li J,Guo W,Zhang Y,Guan F,Ma S

Thu Jun 01 00:00:00 EDT 2023

PINK1 deficiency in gastric cancer compromises mitophagy, promotes the Warburg effect, and facilitates M2 polarization of macrophages.

Cancer letters

Xu Y,Lu J,Tang Y,Xie W,Zhang H,Wang B,Zhang S,Hou W,Zou C,Jiang P,Zhang W

Thu Mar 31 00:00:00 EDT 2022

PINK1 deficiency in gastric cancer compromises mitophagy, promotes the Warburg effect, and facilitates M2 polarization of macrophages.

Cancer letters

Xu Y,Lu J,Tang Y,Xie W,Zhang H,Wang B,Zhang S,Hou W,Zou C,Jiang P,Zhang W

Thu Mar 31 00:00:00 EDT 2022

CircSEMA4B inhibits the progression of breast cancer by encoding a novel protein SEMA4B-211aa and regulating AKT phosphorylation.

Cell death & disease

Wang X,Jian W,Luo Q,Fang L

Sat Sep 17 00:00:00 EDT 2022

RNA-seq transcriptome profiling of liver regeneration in mice identifies the miR-34b-5p/phosphoinositide-dependent protein kinase 1 axis as a potential target for hepatocyte proliferation.

Biochemical and biophysical research communications

Lei X,Dai X,Wang Q,Long R,Xiang Z,Li H,Long Z,Zhang C,Zhu Z

Sun Oct 30 00:00:00 EDT 2022

Honokiol Inhibits HIF-1α-Mediated Glycolysis to Halt Breast Cancer Growth.

Frontiers in pharmacology

Yi X,Qi M,Huang M,Zhou S,Xiong J

Fri Apr 01 00:00:00 EDT 2022

FOXM1 regulates glycolysis in nasopharyngeal carcinoma cells through PDK1.

Journal of cellular and molecular medicine

Yang Q,Wu F,Zhang Y,Wang R

Fri Jul 01 00:00:00 EDT 2022

HDAC2- and EZH2-Mediated Histone Modifications Induce PDK1 Expression through miR-148a Downregulation in Breast Cancer Progression and Adriamycin Resistance.

Cancers

Xie Y,Shi Z,Qian Y,Jiang C,Liu W,Liu B,Jiang B

Sat Jul 23 00:00:00 EDT 2022

Polydopamine-mediated graphene oxide and nanohydroxyapatite-incorporated conductive scaffold with an immunomodulatory ability accelerates periodontal bone regeneration in diabetes.

Bioactive materials

Li Y,Yang L,Hou Y,Zhang Z,Chen M,Wang M,Liu J,Wang J,Zhao Z,Xie C,Lu X

Thu Dec 01 00:00:00 EST 2022

The anti-rotavirus effect of baicalin via the gluconeogenesis-related p-JNK-PDK1-AKT-SIK2 signaling pathway.

European journal of pharmacology

Song L,Zhong P,Zhu X,Zhou R,Gao M,Lan Q,Chen J,Chen Y,Zhao W

Thu Apr 15 00:00:00 EDT 2021

The SIAH2-NRF1 axis spatially regulates tumor microenvironment remodeling for tumor progression.

Nature communications

Ma B,Cheng H,Mu C,Geng G,Zhao T,Luo Q,Ma K,Chang R,Liu Q,Gao R,Nie J,Xie J,Han J,Chen L,Ma G,Zhu Y,Chen Q

Mon Mar 04 00:00:00 EST 2019

Donor Treatment With a Hypoxia-Inducible Factor-1 Agonist Prevents Donation After Cardiac Death Liver Graft Injury in a Rat Isolated Perfusion Model.

Artificial organs

Zhang X,Liu Z,Xiao Q,Zeng C,Lai CH,Fan X,Ye Q,Wang Y,Xiong Y

Thu Mar 01 00:00:00 EST 2018

Donor Treatment With a Hypoxia-Inducible Factor-1 Agonist Prevents Donation After Cardiac Death Liver Graft Injury in a Rat Isolated Perfusion Model.

Artificial organs

Zhang X,Liu Z,Xiao Q,Zeng C,Lai CH,Fan X,Ye Q,Wang Y,Xiong Y

Thu Mar 01 00:00:00 EST 2018

Impairment of Akt activity by CYP2E1 mediated oxidative stress is involved in chronic ethanol-induced fatty liver.

Redox biology

Zeng T,Zhang CL,Zhao N,Guan MJ,Xiao M,Yang R,Zhao XL,Yu LH,Zhu ZP,Xie KQ

Sun Apr 01 00:00:00 EDT 2018

Impairment of Akt activity by CYP2E1 mediated oxidative stress is involved in chronic ethanol-induced fatty liver.

Redox biology

Zeng T,Zhang CL,Zhao N,Guan MJ,Xiao M,Yang R,Zhao XL,Yu LH,Zhu ZP,Xie KQ

Sun Apr 01 00:00:00 EDT 2018

Defective insulin signaling and the protective effects of dimethyldiguanide during follicular development in the ovaries of polycystic ovary syndrome.

Molecular medicine reports

Wang F,Wang S,Zhang Z,Lin Q,Liu Y,Xiao Y,Xiao K,Wang Z

Fri Dec 01 00:00:00 EST 2017

Bioinformatics

Protein Aliases: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; isoenzyme 1; kinase isoenzyme 1; lipoamide; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; PDH kinase 1; PDK p48; pyruvate dehydrogenase; pyruvate dehydrogenase kinase 1; Pyruvate dehydrogenase kinase isoform 1; pyruvate dehydrogenase kinase, isoenzyme 1; pyruvate dehydrogenase kinase, isozyme 1

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Gene Aliases: B830012B01; D530020C15Rik; PDHK1; PDK1

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UniProt ID: (Human) Q15118, (Mouse) Q8BFP9

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Entrez Gene ID: (Human) 5163, (Rat) 116551, (Mouse) 228026

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Function(s)

protein kinase activity pyruvate dehydrogenase (acetyl-transferring) kinase activity protein binding ATP binding protein serine/threonine kinase activity protein complex binding protein homodimerization activity protein heterodimerization activity nucleotide binding kinase activity transferase activity non-receptor serine/threonine protein kinase

Process(es)

glucose metabolic process protein phosphorylation cell proliferation intrinsic apoptotic signaling pathway in response to oxidative stress regulation of acetyl-CoA biosynthetic process from pyruvate regulation of glucose metabolic process hypoxia-inducible factor-1alpha signaling pathway mitophagy in response to mitochondrial depolarization cell surface receptor signaling pathway peptidyl-serine phosphorylation carbohydrate metabolic process phosphorylation intracellular signal transduction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PDK1 Polyclonal Antibody

PDK1 Antibody (18262-1-AP) in ICC/IF

Product Details

18262-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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