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Invitrogen
PDK1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
PDK1 Antibody (PA1-16945) in ICC/IF
Immunofluorescence analysis of PDK1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PDK1 Polyclonal Antibody (Product # PA1-16945) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a d... View More
Product Details
PA1-16945
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide between amino acids 300-400 of the human PDK1 protein.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_793042
Product Specific Information
Suggested positive control: human heart protein, antigen standard for PDK1 (transient overexpression lysate).
Target Information
PDK1 (3-phosphoinositide dependent kinase-1) is a serine/threonine protein kinase localized to the plasma membrane. It plays a vital role in insulin-induced glycogen synthesis, PKC activation, and activation of the AGC subfamily of protein kinases. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; isoenzyme 1; kinase isoenzyme 1; lipoamide; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; PDH kinase 1; pyruvate dehydrogenase; Pyruvate dehydrogenase kinase isoform 1; pyruvate dehydrogenase kinase, isoenzyme 1; pyruvate dehydrogenase kinase, isozyme 1
Gene Aliases: B830012B01; D530020C15Rik; PDHK1; PDK1
UniProt ID: (Human) Q15118, (Mouse) Q8BFP9
Entrez Gene ID: (Human) 5163, (Mouse) 228026
protein kinase activity
pyruvate dehydrogenase (acetyl-transferring) kinase activity
protein binding
ATP binding
nucleotide binding
protein serine/threonine kinase activity
kinase activity
transferase activity
protein complex binding
protein homodimerization activity
protein heterodimerization activity
non-receptor serine/threonine protein kinase
glucose metabolic process
protein phosphorylation
cell proliferation
intrinsic apoptotic signaling pathway in response to oxidative stress
regulation of acetyl-CoA biosynthetic process from pyruvate
regulation of glucose metabolic process
hypoxia-inducible factor-1alpha signaling pathway
mitophagy in response to mitochondrial depolarization
carbohydrate metabolic process
cell surface receptor signaling pathway
phosphorylation
peptidyl-serine phosphorylation
intracellular signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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