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Invitrogen
PDK1 Recombinant Rabbit Monoclonal Antibody (JA67-30)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 10
PDK1 Antibody (MA5-32702) in ICC/IF
Immunofluorescence analysis of PDK1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PDK1 Recombinant Rabbit Monoclonal Antibody (Product # PA5-32702) at 1:100 in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjug... View More
Product Details
MA5-32702
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JA67-30
Immunogen
Synthetic peptide within Human PDK1 aa 16-65
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809979
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
PDK1 (3-phosphoinositide dependent kinase-1) is a serine/threonine protein kinase localized to the plasma membrane. It plays a vital role in insulin-induced glycogen synthesis, PKC activation, and activation of the AGC subfamily of protein kinases. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; isoenzyme 1; kinase isoenzyme 1; lipoamide; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; PDH kinase 1; PDK p48; pyruvate dehydrogenase; pyruvate dehydrogenase kinase 1; Pyruvate dehydrogenase kinase isoform 1; pyruvate dehydrogenase kinase, isoenzyme 1; pyruvate dehydrogenase kinase, isozyme 1
Gene Aliases: B830012B01; D530020C15Rik; PDHK1; PDK1
UniProt ID: (Human) Q15118, (Mouse) Q8BFP9
Entrez Gene ID: (Human) 5163, (Rat) 116551, (Mouse) 228026
protein kinase activity
pyruvate dehydrogenase (acetyl-transferring) kinase activity
protein binding
ATP binding
protein serine/threonine kinase activity
protein complex binding
protein homodimerization activity
protein heterodimerization activity
nucleotide binding
kinase activity
transferase activity
non-receptor serine/threonine protein kinase
glucose metabolic process
protein phosphorylation
cell proliferation
intrinsic apoptotic signaling pathway in response to oxidative stress
regulation of acetyl-CoA biosynthetic process from pyruvate
regulation of glucose metabolic process
hypoxia-inducible factor-1alpha signaling pathway
mitophagy in response to mitochondrial depolarization
cell surface receptor signaling pathway
peptidyl-serine phosphorylation
carbohydrate metabolic process
phosphorylation
intracellular signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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