Proteintech

PDK4 Polyclonal Antibody

PDK4 Antibody in Immunocytochemistry (ICC/IF)

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PDK4 Antibody (12949-1-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 12949-1-AP (PDK4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PDK4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

12949-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

View 39 publications 39 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PDK4 Fusion Protein Ag3629 (61-412 aa encoded by BC040239)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LPVRLANIL KEIDILPTQL VNTSSVQLVK SWYIQSLMDL VEFHEKSPDD QKALSDFVDT LIKVRNRHHN VVPTMAQGII EYKDACTVDP VTNQNLQYFL DRFYMNRIST RMLMNQHILI FSDSQTGNPS HIGSIDPNCD VVAVVQDAFE CSRMLCDQYY LSSPELKLTQ VNGKFPDQPI HIVYVPSHLH HMLFELFKNA MRATVEHQEN QPSLTPIEVI VVLGKEDLTI KISDRGGGVP LRIIDRLFSY TYSTAPTPVM DNSRNAPLAG FGYGLPISRL YAKYFQGDLN LYSLSGYGTD AIIYLKALSS ESIEKLPVFN KSAFKHYQMS SEADDWCIPS REPKNLAKEV AM (61-412 aa encoded by BC040239)

Target Information

PDK4 (PDHK4), along with the other PDHKs, regulates glucose metabolism by phosphorylating the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex. Inhibition of PDHKs is viewed as a potential therapeutic treatment for type II diabetes. This protein is located in the matrix of the mitrochondria and it's expression is regulated by glucocorticoids, retinoic acid and insulin. PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial; kinase isozyme 4; lipoamide; pyruvate dehydrogenase; pyruvate dehydrogenase kinase 4; Pyruvate dehydrogenase kinase isoform 4; pyruvate dehydrogenase kinase, isoenzyme 4; pyruvate dehydrogenase kinase, isozyme 4; pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial; pyruvate dehydrogenate kinase 4

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Gene Aliases: AV005916; PDHK4; PDK4

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UniProt ID: (Human) Q16654, (Rat) O54937, (Mouse) O70571

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Entrez Gene ID: (Human) 5166, (Rat) 89813, (Mouse) 27273

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Function(s)

protein kinase activity pyruvate dehydrogenase (acetyl-transferring) kinase activity ATP binding nucleotide binding protein serine/threonine/tyrosine kinase activity kinase activity transferase activity non-receptor serine/threonine protein kinase

Process(es)

positive regulation of defense response to virus by host glucose metabolic process protein phosphorylation regulation of pH insulin receptor signaling pathway cellular response to starvation regulation of acetyl-CoA biosynthetic process from pyruvate regulation of cellular ketone metabolic process regulation of glucose metabolic process regulation of fatty acid biosynthetic process glucose homeostasis response to starvation regulation of bone resorption regulation of fatty acid oxidation cellular response to fatty acid reactive oxygen species metabolic process xenophagy negative regulation of anoikis acetyl-CoA biosynthetic process from pyruvate carbohydrate metabolic process phosphorylation

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PDK4 Polyclonal Antibody

PDK4 Antibody (12949-1-AP) in ICC/IF

Product Details

12949-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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