Invitrogen

PEBP1 Polyclonal Antibody

PEBP1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

PEBP1 Antibody (36-0700) in ICC/IF

Immunofluorescence analysis of RKIP (N-term) was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PEBP1 Rabbit Polyclonal Antibody (Product # 36-0700) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More

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Product Details

36-0700

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Dog, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the N-terminal region of human RKIP (Raf kinase inhibitor protein).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533236

Target Information

PBP binds ATP, opioids and phosphatidylethanolamine, exhibiting a lower affinity for phosphatidylinositol and phosphatidylcholine. This serine protease inhibitor inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. PBP contains hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Changes of RAS Pathway Phosphorylation in Lymphoblastoid Cell Lines from Noonan Syndrome Patients Carrying Hypomorphic Variants in Two NS Genes.

International journal of molecular sciences

Tritto V,Capitanio D,Gelfi C,Riva P

36-0700 was used in Western Blotting to suggest that the presence of one subclinical variant can activate the RAS pathway below the pathological threshold, which can instead be exceeded by the additive effect due to the co-presence of two subclinical variants causing NS, supporting our digenic inheritance hypothesis.

Fri Feb 17 00:00:00 EST 2023

Changes of RAS Pathway Phosphorylation in Lymphoblastoid Cell Lines from Noonan Syndrome Patients Carrying Hypomorphic Variants in Two NS Genes.

International journal of molecular sciences

Tritto V,Capitanio D,Gelfi C,Riva P

Fri Feb 17 00:00:00 EST 2023

Phospholipid remodeling is critical for stem cell pluripotency by facilitating mesenchymal-to-epithelial transition.

Science advances

Wu Y,Chen K,Xing G,Li L,Ma B,Hu Z,Duan L,Liu X

36-0700 was used in Immunocytochemistry-immunoflourescence to reveal dynamic changes in phospholipids occurring during reprogramming and show that the CDP-ethanolamine (CDP-Etn) pathway for phosphatidylethanolamine (PE) synthesis is required at the early stage of reprogramming.

Fri Nov 01 00:00:00 EDT 2019

Phospholipid remodeling is critical for stem cell pluripotency by facilitating mesenchymal-to-epithelial transition.

Science advances

Wu Y,Chen K,Xing G,Li L,Ma B,Hu Z,Duan L,Liu X

Fri Nov 01 00:00:00 EDT 2019

Phospholipid remodeling is critical for stem cell pluripotency by facilitating mesenchymal-to-epithelial transition.

Science advances

Wu Y,Chen K,Xing G,Li L,Ma B,Hu Z,Duan L,Liu X

Fri Nov 01 00:00:00 EDT 2019

Mapping protein interactions of sodium channel Na_V1.7 using epitope-tagged gene-targeted mice.

The EMBO journal

Kanellopoulos AH,Koenig J,Huang H,Pyrski M,Millet Q,Lolignier S,Morohashi T,Gossage SJ,Jay M,Linley JE,Baskozos G,Kessler BM,Cox JJ,Dolphin AC,Zufall F,Wood JN,Zhao J

Published figure using PEBP1 polyclonal antibody (Product # 36-0700) in Western Blot

Thu Feb 01 00:00:00 EST 2018

Mapping protein interactions of sodium channel Na_V1.7 using epitope-tagged gene-targeted mice.

The EMBO journal

Kanellopoulos AH,Koenig J,Huang H,Pyrski M,Millet Q,Lolignier S,Morohashi T,Gossage SJ,Jay M,Linley JE,Baskozos G,Kessler BM,Cox JJ,Dolphin AC,Zufall F,Wood JN,Zhao J

Published figure using PEBP1 polyclonal antibody (Product # 36-0700) in Western Blot

Thu Feb 01 00:00:00 EST 2018

Effects of Raf kinase inhibitor protein expression on pancreatic cancer cell growth and motility: an in vivo and in vitro study.

Journal of cancer research and clinical oncology

Dai H,Chen H,Liu W,You Y,Tan J,Yang A,Lai X,Bie P

360700 was used in immunohistochemistry - paraffin section to evaluate the effects of Raf kinase inhibitor protein on pancreatic carcinoma cells

Sat Oct 01 00:00:00 EDT 2016

Polycomb protein EZH2 regulates tumor invasion via the transcriptional repression of the metastasis suppressor RKIP in breast and prostate cancer.

Cancer research

Ren G,Baritaki S,Marathe H,Feng J,Park S,Beach S,Bazeley PS,Beshir AB,Fenteany G,Mehra R,Daignault S,Al-Mulla F,Keller E,Bonavida B,de la Serna I,Yeung KC

36-0700 was used in immunohistochemistry and western blot to investigate the role of polycomb protein EZH2 in tumor invasion

Fri Jun 15 00:00:00 EDT 2012

Raf kinase inhibitor protein positively regulates cell-substratum adhesion while negatively regulating cell-cell adhesion.

Journal of cellular biochemistry

Mc Henry KT,Montesano R,Zhu S,Beshir AB,Tang HH,Yeung KC,Fenteany G

Fri Feb 15 00:00:00 EST 2008

Raf kinase inhibitor protein positively regulates cell-substratum adhesion while negatively regulating cell-cell adhesion.

Journal of cellular biochemistry

Mc Henry KT,Montesano R,Zhu S,Beshir AB,Tang HH,Yeung KC,Fenteany G

Fri Feb 15 00:00:00 EST 2008

Raf kinase inhibitor protein positively regulates cell-substratum adhesion while negatively regulating cell-cell adhesion.

Journal of cellular biochemistry

Mc Henry KT,Montesano R,Zhu S,Beshir AB,Tang HH,Yeung KC,Fenteany G

Fri Feb 15 00:00:00 EST 2008

Raf kinase inhibitor protein positively regulates cell-substratum adhesion while negatively regulating cell-cell adhesion.

Journal of cellular biochemistry

Mc Henry KT,Montesano R,Zhu S,Beshir AB,Tang HH,Yeung KC,Fenteany G

Fri Feb 15 00:00:00 EST 2008

Rituximab (chimeric anti-CD20 monoclonal antibody) inhibits the constitutive nuclear factor-{kappa}B signaling pathway in non-Hodgkin's lymphoma B-cell lines: role in sensitization to chemotherapeutic drug-induced apoptosis.

Cancer research

Jazirehi AR,Huerta-Yepez S,Cheng G,Bonavida B

Sat Jan 01 00:00:00 EST 2005

Inhibition of the Raf-MEK1/2-ERK1/2 signaling pathway, Bcl-xL down-regulation, and chemosensitization of non-Hodgkin's lymphoma B cells by Rituximab.

Cancer research

Jazirehi AR,Vega MI,Chatterjee D,Goodglick L,Bonavida B

Fri Oct 01 00:00:00 EDT 2004

Inhibition of the Raf-MEK1/2-ERK1/2 signaling pathway, Bcl-xL down-regulation, and chemosensitization of non-Hodgkin's lymphoma B cells by Rituximab.

Cancer research

Jazirehi AR,Vega MI,Chatterjee D,Goodglick L,Bonavida B

Fri Oct 01 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: 23 kDa morphine-binding protein; epididymis luminal protein 210; epididymis secretory protein Li 34; epididymis secretory protein Li 96; HCNPpp; hippocampal cholinergic neurostimulating peptide; KIAA0299; MOCA; Neuropolypeptide h3; P23K; PEBP-1; Phosphatidylethanolamine-binding protein 1; PPBP; prostatic binding protein; Prostatic-binding protein; Raf kinase inhibitor protein; Raf kinase inhibitory protein; Raf-1 inhibitor protein; Raf-1 kinase inhibitor protein; RKIP

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Gene Aliases: HCNP; HCNPpp; HEL-210; HEL-S-34; HEL-S-96; PBP; Pbp1; Pbpr; PEBP; PEBP-1; PEBP1; RKIP

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UniProt ID: (Human) P30086, (Rat) P31044, (Mouse) P70296

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Entrez Gene ID: (Human) 5037, (Rat) 29542, (Mouse) 23980

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Function(s)

serine-type endopeptidase inhibitor activity protein binding ATP binding phosphatidylethanolamine binding enzyme binding protein kinase binding poly(A) RNA binding receptor binding lipid binding kinase binding mitogen-activated protein kinase binding nucleotide binding peptidase inhibitor activity protease inhibitor

Process(es)

MAPK cascade negative regulation of endopeptidase activity regulation of neurotransmitter levels negative regulation of protein phosphorylation regulation of the force of heart contraction response to oxidative stress spermatid development brain development aging response to heat response to wounding response to toxic substance response to organic substance response to organonitrogen compound response to organic cyclic compound response to activity response to drug eating behavior negative regulation of MAPK cascade positive regulation of cAMP-mediated signaling response to ethanol positive regulation of mitotic nuclear division sperm capacitation response to corticosterone response to cAMP response to calcium ion response to electrical stimulus positive regulation of acetylcholine metabolic process negative regulation of peptidase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PEBP1 Polyclonal Antibody

PEBP1 Antibody (36-0700) in ICC/IF

Product Details

36-0700

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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