Invitrogen

PERK Monoclonal Antibody (5G5)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

2 References

View all (33) PERK antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 4

PERK Antibody (MA5-15705) in WB

Western blot analysis of EIF2AK3 using EIF2AK3 monoclonal antibody (Product # MA5-15705) in HEK293 (1) and EIF2AK3 (AA: 929-1116) human IgG Fc transfected HEK293 (2) cell lysate.

Product Image Gallery

PERK Antibody — Figure 3. Combination of LEB and I-131 regulates apoptosis of nasopharyngeal carcinoma cells through ER stress. (A) ATF6, IER1, RERK and CHOP expression levels in HK-1 cells following treatment with LEB or/and I-131 determined by western blotting. (B) Analysis of mRNA expression levels of ATF6, IER1, RERK and CHOP expression levels in HK-1 cells. (C) Effects of LEB or/and I-131 on ER in HK-1 cells (magnification, x100). (D) GRP78 expression in HK-1 cells following combined treatment of LEB and I-131 determined by immunofluorescence (magnification, x20). (E) Adhered morphology of HK-1 cells following treatment with LEB or/and I-131 (magnification, x20). (F) JNK and P38 expression levels in HK-1 following treatment with LEB or/and I-131. The data are presented as the mean + standard error of the mean of three independent experiments. **P<0.01. LEB, Lenvatinib; I-131, iodine-131; ATF6, activating transcription factor 6; IER1, inositol-requiring protein 1; RERK, protein kinase RNA-like endoplasmic reticulum kinase; CHOP, C/EBP homologous protein; GRP78, glucose-regulated protein 78; JNK, c-jun N-terminal kinase. {TM}

Product Details

MA5-15705

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5G5

Immunogen

Purified recombinant fragment of human EIF2AK3 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10986057

Product Specific Information

MA5-15705 targets EIF2AK3 in indirect ELISA, WB applications and shows reactivity with Human samples.

The MA5-15705 immunogen is purified recombinant fragment of human EIF2AK3 expressed in E. Coli.

MA5-15705 detects EIF2AK3 which has a predicted molecular weight of approximately 125kDa.

Target Information

EIF2AK3 (PERK) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK3, signaling is initiated by misfolded proteins in the ER. Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS), also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pulsed focused ultrasound enhances the therapeutic effect of mesenchymal stromal cell-derived extracellular vesicles in acute kidney injury.

Stem cell research & therapy

Ullah M,Liu DD,Rai S,Razavi M,Concepcion W,Thakor AS

MA5-15705 was used in Western Blotting to show that pFUS may be a strategy for enhancing the therapeutic efficacy of MSC-derived EV treatment for the treatment of AKI.

Mon Sep 14 00:00:00 EDT 2020

Pulsed focused ultrasound enhances the therapeutic effect of mesenchymal stromal cell-derived extracellular vesicles in acute kidney injury.

Stem cell research & therapy

Ullah M,Liu DD,Rai S,Razavi M,Concepcion W,Thakor AS

MA5-15705 was used in Western Blotting to show that pFUS may be a strategy for enhancing the therapeutic efficacy of MSC-derived EV treatment for the treatment of AKI.

Mon Sep 14 00:00:00 EDT 2020

Combined effects of Lenvatinib and iodine-131 on cell apoptosis in nasopharyngeal carcinoma through inducing endoplasmic reticulum stress.

Experimental and therapeutic medicine

Wang G,Zhuang J,Ni J,Ye Y,He S,Xia W

Published figure using PERK monoclonal antibody (Product # MA5-15705) in Western Blot

Mon Oct 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: 2.7.11.1; Eukaryotic translation initiation factor 2-alpha kinase 3; HsPEK; OTTHUMP00000207187; OTTHUMP00000207188; Pancreatic eIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase

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Gene Aliases: EIF2AK3; PEK; PERK; WRS

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UniProt ID: (Human) Q9NZJ5

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Entrez Gene ID: (Human) 9451

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Function(s)

protein kinase activity protein serine/threonine kinase activity eukaryotic translation initiation factor 2alpha kinase activity protein binding ATP binding enzyme binding protein phosphatase binding identical protein binding protein homodimerization activity Hsp90 protein binding non-receptor serine/threonine protein kinase

Process(es)

skeletal system development ossification angiogenesis chondrocyte development protein phosphorylation activation of cysteine-type endopeptidase activity involved in apoptotic process ER overload response endoplasmic reticulum organization positive regulation of vascular endothelial growth factor production positive regulation of gene expression regulation of translational initiation by eIF2 alpha phosphorylation negative regulation of translation peptidyl-serine phosphorylation calcium-mediated signaling insulin secretion bone mineralization endoplasmic reticulum unfolded protein response endocrine pancreas development negative regulation of myelination negative regulation of translational initiation in response to stress cellular response to amino acid starvation response to endoplasmic reticulum stress eiF2alpha phosphorylation in response to endoplasmic reticulum stress PERK-mediated unfolded protein response cellular response to glucose starvation positive regulation of transcription from RNA polymerase I promoter protein autophosphorylation insulin-like growth factor receptor signaling pathway protein homooligomerization regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation cellular response to cold positive regulation of protein localization to nucleus regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway response to manganese-induced endoplasmic reticulum stress

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PERK Monoclonal Antibody (5G5)

PERK Antibody (MA5-15705) in WB

Product Details

MA5-15705

Western Blot (WB)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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