Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
PF4 Antibody (PA5-79810) in IHC (P)
Immunohistochemistry analysis of PF4 on paraffin-embedded mouse spleen tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with PF4 polyclonal antibody (Product# PA5-79810) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development... View More
Product Details
PA5-79810
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived rat PF4 recombinant protein (Position: V30-S105).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2746925
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4; PF-4; Platelet factor 4
Gene Aliases: Cxcl4; PF-4; Pf4; Pf4a; RATPF4A; Scyb4
UniProt ID: (Rat) P06765, (Mouse) Q9Z126
Entrez Gene ID: (Rat) 360918, (Mouse) 56744
positive regulation of leukocyte chemotaxis
protein complex assembly
inflammatory response
immune response
G-protein coupled receptor signaling pathway
positive regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
negative regulation of angiogenesis
cytokine-mediated signaling pathway
platelet activation
leukocyte chemotaxis
positive regulation of cAMP metabolic process
response to lipopolysaccharide
positive regulation of tumor necrosis factor production
regulation of cell proliferation
positive regulation of cAMP-mediated signaling
negative regulation of MHC class II biosynthetic process
positive regulation of macrophage differentiation
negative regulation of megakaryocyte differentiation
negative regulation of cytolysis
positive regulation of transcription from RNA polymerase II promoter
chemokine-mediated signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
chemotaxis
cell chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support