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PFKM Antibody (PA5-29336) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of C2C12 (Lane 1), HeLa (Lane 2), A-431 (Lane 3), NIH/3T3 (Lane 4) and HEK 293 (Lane 5). The blot was probed with Anti-PFKM Polyclonal Antibody (Product # PA5-29336, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 85 kDa band corresponding to PFKM was observed across the cell lines tested.
Product Details
PA5-29336
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 424 and 761 of Human PFK
Conjugate
Form
Liquid
Concentration
0.57 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2546812
Product Specific Information
Recommended positive controls: 293T, A431, H1299, mouse brain, rat brain.
Predicted reactivity: Mouse (98%), Rat (96%), Xenopus laevis (81%), Dog (99%), Pig (97%), Chicken (82%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 6-phosphofructo-1-kinase; 6-phosphofructokinase type A; 6-phosphofructokinase, muscle type; ATP-dependent 6-phosphofructokinase, muscle type; ATP-dependent 6-phosphofructokinase, muscle type {ECO:0000255|HAMAP-Rule:MF_03184}; ATP-PFK; ATP-PFK {ECO:0000255|HAMAP-Rule:MF_03184}; PFK-A; Phosphofructo-1-kinase isozyme A; phosphofructokinase 1; phosphofructokinase 4; phosphofructokinase M variant 3/4; phosphofructokinase muscle; phosphofructokinase, polypeptide X; phosphofructokinase-1 A isozyme; phosphofructokinase-A; phosphofructokinase-M; phosphofructokinase-M (connecting peptide); Phosphohexokinase; phosphohexokinase {ECO:0000255|HAMAP-Rule:MF_03184}; protein phosphatase 1, regulatory subunit 122
Gene Aliases: AI131669; ATP-PFK; GSD7; PFK-1; Pfk-4; PFK-A; PFK-M; PFK1; Pfk4; PFKA; PFKM; PFKX; PPP1R122
UniProt ID: (Human) P08237, (Mouse) P47857, (Rat) P47858
Entrez Gene ID: (Human) 5213, (Mouse) 18642, (Rat) 65152
6-phosphofructokinase activity
protein binding
ATP binding
protein C-terminus binding
kinase binding
identical protein binding
protein homodimerization activity
metal ion binding
fructose binding
nucleotide binding
catalytic activity
phosphofructokinase activity
AMP binding
kinase activity
transferase activity
carbohydrate binding
fructose-6-phosphate binding
carbohydrate kinase
kinase
transferase
metabolite interconversion enzyme
glycogen catabolic process
fructose 6-phosphate metabolic process
glycolytic process
positive regulation of insulin secretion
glucose homeostasis
muscle cell cellular homeostasis
carbohydrate phosphorylation
protein oligomerization
canonical glycolysis
glycolysis from storage polysaccharide through glucose-1-phosphate
metabolic process
phosphorylation
fructose 1,6-bisphosphate metabolic process
protein homotetramerization
glycolytic process through fructose-6-phosphate
cellular glucose homeostasis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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