Proteintech

PGAM1 Polyclonal Antibody

PGAM1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

PGAM1 Antibody (16126-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed A549 cells using 16126-1-AP (PGAM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PGAM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

16126-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PGAM1 Fusion Protein Ag9110 (1-254 aa encoded by BC011678)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is specific to PGAM1.

Immunogen sequence: MAAYKLVLI RHGESAWNLE NRFSGWYDAD LSPAGHEEAK RGGQALRDAG YEFDICFTSV QKRAIRTLWT VLDAIDQMWL PVVRTWRLNE RHYGGLTGLN KAETAAKHGE AQVKIWRRSY DVPPPPMEPD HPFYSNISKD RRYADLTEDQ LPSCESLKDT IARALPFWNE EIVPQIKEGK RVLIAAHGNS LRGIVKHLEG LSEEAIMELN LPTGIPIVYE LDKNLKPIKP MQFLGDEETV RKAMEAVAAQ GKAKK (1-254 aa encoded by BC011678)

Target Information

PGAM1 belongs to the phosphoglycerate mutase family. PGAM1 is an important component of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM1 protein is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Mutations in PGAM1 cause muscle phosphoglycerate mutase efficiency, also known as glycogen storage disease X. Recombinant human PGAM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Auger C,Moudgalya H,Neely MR,Stephan JT,Tarhoni I,Gerard D,Basu S,Fhied CL,Abdelkader A,Vargas M,Hu S,Hulett T,Liptay MJ,Shah P,Seder CW,Borgia JA

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C11orf54 promotes DNA repair via blocking CMA-mediated degradation of HIF1A.

Communications biology

Tan J,Wang W,Liu X,Xu J,Che Y,Liu Y,Hu J,Hu L,Li J,Zhou Q

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Ubiquitin specific protease 46 potentiates triple negative breast cancer development by stabilizing PGAM1-mediated glycolysis.

Cell biology international

Ke L,Jia Z,Gao W,Luo L

Sun Jan 01 00:00:00 EST 2023

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Molecular cell

Zhu Y,Jin L,Shi R,Li J,Wang Y,Zhang L,Liang CZ,Narayana VK,De Souza DP,Thorne RF,Zhang LR,Zhang XD,Wu M

Thu Feb 03 00:00:00 EST 2022

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

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Zhu Y,Jin L,Shi R,Li J,Wang Y,Zhang L,Liang CZ,Narayana VK,De Souza DP,Thorne RF,Zhang LR,Zhang XD,Wu M

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MYB proto-oncogene like 2 promotes hepatocellular carcinoma growth and glycolysis via binding to the Optic atrophy 3 promoter and activating its expression.

Bioengineered

Liu M,Du Q,Mao G,Dai N,Zhang F

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Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang X,Luo F,Luo S,Li L,Ren X,Lin J,Liang Y,Ma C,Ding L,Zhang D,Ye T,Lin Y,Jin B,Gao S,Ye Q

Thu Sep 01 00:00:00 EDT 2022

U2 small nuclear RNA auxiliary factor 2, transcriptionally activated by the transcription factor Dp-1/E2F transcription factor 1 complex, enhances the growth and aerobic glycolysis of leiomyosarcoma cells.

Bioengineered

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Fri Apr 01 00:00:00 EDT 2022

Progranulin-derived granulin E and lysosome membrane protein CD68 interact to reciprocally regulate their protein homeostasis.

The Journal of biological chemistry

Santos MN,Paushter DH,Zhang T,Wu X,Feng T,Lou J,Du H,Becker SM,Fragoza R,Yu H,Hu F

Thu Sep 01 00:00:00 EDT 2022

A Schistosoma japonicum MicroRNA Exerts Antitumor Effects Through Inhibition of Both Cell Migration and Angiogenesis by Targeting PGAM1.

Frontiers in oncology

Hu C,Li Y,Pan D,Wang J,Zhu L,Lin Y,Zhu S,Pan W

Tue Jul 06 00:00:00 EDT 2021

Histone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis.

EMBO reports

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EMBO reports

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Valproic acid Suppresses Breast Cancer Cell Growth Through Triggering Pyruvate Kinase M2 Isoform Mediated Warburg Effect.

Cell transplantation

Li Z,Yang L,Zhang S,Song J,Sun H,Shan C,Wang D,Liu S

Tue Feb 01 00:00:00 EST 2022

Reciprocal regulation of LOXL2 and HIF1α drives the Warburg effect to support pancreatic cancer aggressiveness.

Cell death & disease

Li R,Li H,Zhu L,Zhang X,Liu D,Li Q,Ni B,Hu L,Zhang Z,Zhang Y,Wang X,Jiang SH

Fri Nov 26 00:00:00 EST 2021

Enhancer Reprogramming Confers Dependence on Glycolysis and IGF Signaling in KMT2D Mutant Melanoma.

Cell reports

Maitituoheti M,Keung EZ,Tang M,Yan L,Alam H,Han G,Singh AK,Raman AT,Terranova C,Sarkar S,Orouji E,Amin SB,Sharma S,Williams M,Samant NS,Dhamdhere M,Zheng N,Shah T,Shah A,Axelrad JB,Anvar NE,Lin YH,Jiang S,Chang EQ,Ingram DR,Wang WL,Lazar A,Lee MG,Muller F,Wang L,Ying H,Rai K

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Li L,Liang Y,Kang L,Liu Y,Gao S,Chen S,Li Y,You W,Dong Q,Hong T,Yan Z,Jin S,Wang T,Zhao W,Mai H,Huang J,Han X,Ji Q,Song Q,Yang C,Zhao S,Xu X,Ye Q

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Cancer cell

Li L,Liang Y,Kang L,Liu Y,Gao S,Chen S,Li Y,You W,Dong Q,Hong T,Yan Z,Jin S,Wang T,Zhao W,Mai H,Huang J,Han X,Ji Q,Song Q,Yang C,Zhao S,Xu X,Ye Q

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Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.

The EMBO journal

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Mon May 15 00:00:00 EDT 2017

Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.

The EMBO journal

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Sun L,Song L,Wan Q,Wu G,Li X,Wang Y,Wang J,Liu Z,Zhong X,He X,Shen S,Pan X,Li A,Wang Y,Gao P,Tang H,Zhang H

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Cell research

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Bioinformatics

Protein Aliases: BPG-dependent PGAM 1; epididymis secretory protein Li 35; PGAM-B; Phosphoglycerate mutase 1; phosphoglycerate mutase 1 (brain); phosphoglycerate mutase A, nonmuscle form; Phosphoglycerate mutase isozyme B; RP11-452K12.8

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Gene Aliases: 2310050F24Rik; CDABP0006; HEL-S-35; Pgam-1; PGAM-B; PGAM1; PGAMA; Pgmut

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UniProt ID: (Human) P18669, (Rat) P25113, (Mouse) Q9DBJ1

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Entrez Gene ID: (Human) 5223, (Rat) 24642, (Mouse) 18648

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Function(s)

bisphosphoglycerate mutase activity bisphosphoglycerate 2-phosphatase activity phosphoglycerate mutase activity protein binding protein kinase binding 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity catalytic activity hydrolase activity isomerase activity intramolecular transferase activity, phosphotransferases mutase

Process(es)

gluconeogenesis glycolytic process regulation of glycolytic process dephosphorylation regulation of pentose-phosphate shunt respiratory burst canonical glycolysis metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PGAM1 Polyclonal Antibody

PGAM1 Antibody (16126-1-AP) in ICC/IF

Product Details

16126-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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