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PGF Antibody (PA5-79814) in WB
Western blot analysis of PGF in Lane 1: rat gastric tissue lysate, Lane 2: rat liver tissue lysate, Lane 3: mouse gastric tissue lysate, Lane 4: mouse liver tissue lysate, Lane 5: mouse HEPA1-6 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with ... View More
Product Details
PA5-79814
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived rat PGF recombinant protein (Position: A24-L158).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2746929
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Placental growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family. PlGF is a pleiotropic factor affects different cell types and regulates various biological responses. The activity of vascular endothelial growth factor (VEGF), which is the most potent of all angiogenic mediators, is partly modulated by PlGF. It plays a major role in vasculogenesis and angiogenesis in human placenta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Placenta growth factor; PlGF; PlGF 1; SHGC
Gene Aliases: AI854365; Pgf; PIGF; Plgf
UniProt ID: (Mouse) P49764, (Rat) Q63434
Entrez Gene ID: (Mouse) 18654, (Rat) 94203
angiogenesis
branching involved in ureteric bud morphogenesis
positive regulation of endothelial cell proliferation
sprouting angiogenesis
multicellular organism development
positive regulation of cell proliferation
cell differentiation
positive regulation of angiogenesis
positive regulation of cell division
regulation of morphogenesis of a branching structure
response to hypoxia
female pregnancy
organ regeneration
cellular response to hormone stimulus
response to drug
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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