Invitrogen

PGP9.5 Monoclonal Antibody (31A3)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

10 Published Figures

17 References

View all (166) PGP9.5 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

PGP9.5 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

PGP9.5 Antibody (MA1-83428) in ICC/IF

Immunofluorescence analysis of PGP9.5 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PGP9.5 Monoclonal Antibody (31A3) (Product # MA1-83428) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a d... View More

PGP9.5 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

PGP9.5 Antibody in Immunohistochemistry (IHC)

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Product Details

MA1-83428

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

5 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

1:500-1:2,000

Product Specifications

Species Reactivity

Human, Mouse, Rabbit, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

31A3

Immunogen

Native protein, from brain.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_935198

Product Specific Information

This antibody does not react with guinea pig.

This antibody is suitable for use on paraffin-embedded tissue sections, with a recommend fixation in 95% ethanol/5% acetic acid for 2-3 hours prior to paraffin-embedding. Specimens which have not been fixed in acetic acid/alcohol will likely require pretreatment using the microwave-citrate buffer method.

Mouse anti Human Protein Gene Product 9.5 antibody, clone 31A3 recognizes protein gene product 9.5 (PGP9.5), a ubiquitin hydrolase which is widely expressed in neuronal tissues and represents 1-2% of total soluble brain proteins.

Target Information

PGP9.5 (Protein gene product 9.5, UCH-L1, PARK5) is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. PGP9.5 has a molecular weight of 27 kDa and was first identified by high resolution two dimensional PAGE. PGP9.5 is a member of ubiquitin carboxyl-terminal hydrolase family 1 (peptidase family C12) with a ubiquitin carboxyl-terminal hydrolase domain. PGP9.5 is well known for having ubiquitin hydrolase and ligase activities that hydrolyzes small C-terminal adducts of ubiquitin to generate ubiquitin monomers. PGP9.5 is present in neurons and nerve fibers at all levels of the central and peripheral nervous system, in neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. Over expression of PGP9.5 leads to non-small cell lung cancer while decreased expression leads to Huntington disease and Alzheimer disease. Since PGP9.5 is present in cellular inclusions, it can be a useful as a neuronal marker and in the studies of neurodegenerative disorders such as with Parkinson disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

LRRK2 expression in normal and pathologic human gut and in rodent enteric neural cell lines.

Journal of neurochemistry

de Guilhem de Lataillade A,Caillaud M,Oullier T,Naveilhan P,Pellegrini C,Tolosa E,Neunlist M,Rolli-Derkinderen M,Gelpi E,Derkinderen P

MA1-83428 was used in Immunohistochemistry and Western Blotting to provide a basis to unravel the functions of LRRK2 in the ENS and to further investigate the pathological changes in enteric synucleinopathies.

Sun Jan 01 00:00:00 EST 2023

LRRK2 expression in normal and pathologic human gut and in rodent enteric neural cell lines.

Journal of neurochemistry

de Guilhem de Lataillade A,Caillaud M,Oullier T,Naveilhan P,Pellegrini C,Tolosa E,Neunlist M,Rolli-Derkinderen M,Gelpi E,Derkinderen P

Sun Jan 01 00:00:00 EST 2023

LRRK2 expression in normal and pathologic human gut and in rodent enteric neural cell lines.

Journal of neurochemistry

de Guilhem de Lataillade A,Caillaud M,Oullier T,Naveilhan P,Pellegrini C,Tolosa E,Neunlist M,Rolli-Derkinderen M,Gelpi E,Derkinderen P

Sun Jan 01 00:00:00 EST 2023

Comparison of commercially available antibodies for the detection of phosphorylated alpha-synuclein in primary culture of ENS.

Neurogastroenterology and motility

Lassozé S,de Guilhem de Lataillade A,Oullier T,Neunslist M,Leclair-Visonneau L,Derkinderen P,Paillusson S

MA1-83428 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to evaluate specificity and sensitivity of 5 different antibodies and reveal that rabbit monoclonal antibody D1R1R uniquely, robustly and specifically detects endogenous phosphorylated alpha-synuclein at Ser129 in rat primary culture of ENS.

Wed Jun 01 00:00:00 EDT 2022

Comparison of commercially available antibodies for the detection of phosphorylated alpha-synuclein in primary culture of ENS.

Neurogastroenterology and motility

Lassozé S,de Guilhem de Lataillade A,Oullier T,Neunslist M,Leclair-Visonneau L,Derkinderen P,Paillusson S

Wed Jun 01 00:00:00 EDT 2022

A functional network of highly pure enteric neurons in a dish.

Frontiers in neuroscience

Caillaud M,Le Dréan ME,De-Guilhem-de-Lataillade A,Le Berre-Scoul C,Montnach J,Nedellec S,Loussouarn G,Paillé V,Neunlist M,Boudin H

Published figure using PGP9.5 monoclonal antibody (Product # MA1-83428) in Immunocytochemistry

Wed Feb 01 00:00:00 EST 2023

A functional network of highly pure enteric neurons in a dish.

Frontiers in neuroscience

Caillaud M,Le Dréan ME,De-Guilhem-de-Lataillade A,Le Berre-Scoul C,Montnach J,Nedellec S,Loussouarn G,Paillé V,Neunlist M,Boudin H

Published figure using PGP9.5 monoclonal antibody (Product # MA1-83428) in Immunocytochemistry

Wed Feb 01 00:00:00 EST 2023

Infrapatellar Fat Pad-Synovial Membrane Anatomo-Fuctional Unit: Microscopic Basis for Piezo1/2 Mechanosensors Involvement in Osteoarthritis Pain.

Frontiers in cell and developmental biology

Emmi A,Stocco E,Boscolo-Berto R,Contran M,Belluzzi E,Favero M,Ramonda R,Porzionato A,Ruggieri P,De Caro R,Macchi V

MA1-83428 was used in Immunohistochemistry to investigate the IFP-SM AFU microscopic features in both healthy and pathological conditions, highlighting peculiar differences in the study cohort.

Sat Jul 16 00:00:00 EDT 2022

Infrapatellar Fat Pad-Synovial Membrane Anatomo-Fuctional Unit: Microscopic Basis for Piezo1/2 Mechanosensors Involvement in Osteoarthritis Pain.

Frontiers in cell and developmental biology

Emmi A,Stocco E,Boscolo-Berto R,Contran M,Belluzzi E,Favero M,Ramonda R,Porzionato A,Ruggieri P,De Caro R,Macchi V

MA1-83428 was used in Immunohistochemistry to investigate the IFP-SM AFU microscopic features in both healthy and pathological conditions, highlighting peculiar differences in the study cohort.

Sat Jul 16 00:00:00 EDT 2022

Adhesion of Gastric Cancer Cells to the Enteric Nervous System: Comparison between the Intestinal Type and Diffuse Type of Gastric Cancer.

Cancers

Girot P,Chapelle N,Aymeric L,Bessard A,Prigent A,Touchefeu Y,Varon C,Neunlist M,Duchalais E,Matysiak-Budnik T

MA1-83428 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to suggest a potential role of enteric neurons in the dissemination of GC cells, especially of the diffuse-type, partly through N-Cadherin.

Wed Jul 06 00:00:00 EDT 2022

The ephrin receptor EphB2 regulates the connectivity and activity of enteric neurons.

The Journal of biological chemistry

Bodin R,Paillé V,Oullier T,Durand T,Aubert P,Le Berre-Scoul C,Hulin P,Neunlist M,Cissé M

MA1-83428 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to identify a critical role for EphB2 in the ENS and reveal a unique EphB2-mediated molecular program of synapse regulation in enteric neurons.

Mon Nov 01 00:00:00 EDT 2021

The ephrin receptor EphB2 regulates the connectivity and activity of enteric neurons.

The Journal of biological chemistry

Bodin R,Paillé V,Oullier T,Durand T,Aubert P,Le Berre-Scoul C,Hulin P,Neunlist M,Cissé M

Mon Nov 01 00:00:00 EDT 2021

Mitochondrial apoptosis and curtailment of hypoxia-inducible factor-1α/fatty acid synthase: A dual edge perspective of gamma linolenic acid in ER+ mammary gland cancer.

Cell biochemistry and function

Roy S,Singh M,Rawat A,Kumar D,Kaithwas G

Published figure using PGP9.5 monoclonal antibody (Product # MA1-83428) in Western Blot

Wed Jul 01 00:00:00 EDT 2020

Activation of prolyl hydroxylase-2 for stabilization of mitochondrial stress along with simultaneous downregulation of HIF-1α/FASN in ER + breast cancer subtype.

Cell biochemistry and function

Devi U,Singh M,Roy S,Gupta PS,Ansari MN,Saeedan AS,Kaithwas G

MA1-83428 was used in Western Blot to elaborate the chemical activation of prolyl hydroxylase-2 by which the increased expression of HIF-1α and FASN can be reduced in mammary gland carcinoma.

Sat Jun 01 00:00:00 EDT 2019

Repurposing mechanistic insight of PDE-5 inhibitor in cancer chemoprevention through mitochondrial-oxidative stress intervention and blockade of DuCLOX signalling.

BMC cancer

Singh M,Kasna S,Roy S,Aldosary S,Saeedan AS,Ansari MN,Kaithwas G

Published figure using PGP9.5 monoclonal antibody (Product # MA1-83428) in Western Blot

Thu Oct 24 00:00:00 EDT 2019

Chemical activation of prolyl hydroxylase-2 by BBAP-1 down regulates hypoxia inducible factor-1α and fatty acid synthase for mammary gland chemoprevention.

RSC advances

Singh M,Devi U,Roy S,Gupta PS,Kaithwas G

Published figure using PGP9.5 monoclonal antibody (Product # MA1-83428) in Western Blot

Tue Apr 03 00:00:00 EDT 2018

Comparative efficacy of alpha-linolenic acid and gamma-linolenic acid to attenuate valproic acid-induced autism-like features.

Journal of physiology and biochemistry

Yadav S,Tiwari V,Singh M,Yadav RK,Roy S,Devi U,Gautam S,Rawat JK,Ansari MN,Saeedan AS,Prakash A,Saraf SA,Kaithwas G

MA1-83428 was used in western blot to assess the effect of alpha-linolenic acid and gamma-linolenic acid in an autism model

Mon May 01 00:00:00 EDT 2017

Alpha-linolenic acid stabilizes HIF-1 α and downregulates FASN to promote mitochondrial apoptosis for mammary gland chemoprevention.

Oncotarget

Roy S,Rawat AK,Sammi SR,Devi U,Singh M,Gautam S,Yadav RK,Rawat JK,Singh L,Ansari MN,Saeedan AS,Pandey R,Kumar D,Kaithwas G

Published figure using PGP9.5 monoclonal antibody (Product # MA1-83428) in Western Blot

Tue Sep 19 00:00:00 EDT 2017

α-Chymotrypsin regulates free fatty acids and UCHL-1 to ameliorate N-methyl nitrosourea induced mammary gland carcinoma in albino wistar rats.

Inflammopharmacology

Rani A,Roy S,Singh M,Devi U,Yadav RK,Gautam S,Rawat JK,Ansari MN,Saeedan AS,Prakash A,Kaithwas G

MA1-83428 was used in western blot to determine the effect of alpha-chymotrypsin on methyl nitrosourea-induced mammary gland carcinoma using albino wistar rats

Sat Oct 01 00:00:00 EDT 2016

Effect of β-sitosterol against methyl nitrosourea-induced mammary gland carcinoma in albino rats.

BMC complementary and alternative medicine

Manral C,Roy S,Singh M,Gautam S,Yadav RK,Rawat JK,Devi U,Ansari MN,Saeedan AS,Kaithwas G

MA1-83428 was used in western blot to assess the effects of beta-sitosterol on methyl nitrosourea-induced mammary gland carcinoma using albino wistar rats

Fri Jul 29 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: epididymis luminal protein 117; epididymis secretory protein Li 53; gracile axonal dystrophy; Neuron cytoplasmic protein 9.5; OTTHUMP00000218137; OTTHUMP00000218139; OTTHUMP00000218140; OTTHUMP00000218141; PGP 9.5; PGP9.5; protein gene product 9.5; ubiquitin C-terminal hydrolase; ubiquitin carboxy-terminal hydrolase L1; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); Ubiquitin carboxyl-terminal hydrolase isozyme L1; Ubiquitin thioesterase L1; ubiquitin thiolesterase; UCH-L1; UCHL

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Gene Aliases: AW822034; C88048; gad; HEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; R75593; UCH-L1; UCHL-1; UCHL1

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UniProt ID: (Human) P09936, (Mouse) Q9R0P9, (Rat) Q00981

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Entrez Gene ID: (Human) 7345, (Rabbit) 100357806, (Mouse) 22223, (Rat) 29545

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Function(s)

cysteine-type endopeptidase activity thiol-dependent ubiquitin-specific protease activity protein binding omega peptidase activity ligase activity ubiquitin protein ligase binding alpha-2A adrenergic receptor binding ubiquitin binding peptidase activity cysteine-type peptidase activity hydrolase activity cysteine protease

Process(es)

response to ischemia axon target recognition adult walking behavior cell proliferation regulation of macroautophagy protein deubiquitination sensory perception of pain axonal transport of mitochondrion eating behavior proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of MAP kinase activity muscle fiber development neuromuscular process proteolysis ubiquitin-dependent protein catabolic process axonogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PGP9.5 Monoclonal Antibody (31A3)

PGP9.5 Antibody (MA1-83428) in ICC/IF

Product Details

MA1-83428

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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