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Product Details
PA5-85354
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human PI3 kinase p85 alpha.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792496
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (91%), Rat (89%), Rhesus Monkey (97%), Bovine (93%).
Positive Control: Jurkat, Raji, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Phosphatidylinositol 3-kinase (PIK3) is a signaling protein that phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. Signaling is initiated when upstream tyrosine kinase receptors or G protein coupled receptors are bound by their ligands. It binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. During insulin stimulation, it also binds to IRS-1. Mutations in the gene can result in Agammaglobulinemia 7, SHORT syndrome, or Immunodeficiency 36.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: p55-GAMMA; phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; Phosphatidylinositol 3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase regulatory subunit gamma; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase-associated p-85 alpha; phosphoinositide-3-kinase regulatory subunit; phosphoinositide-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); PI3-kinase p85-subunit alpha; PI3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; PI3K regulatory subunit gamma; polypeptide 1 p85 alpha; ptdIns-3-kinase regulatory subunit alpha; PtdIns-3-kinase regulatory subunit p85-al; RP4-533D7.2
Gene Aliases: AGM7; GRB1; IMD36; p50alpha; p55alpha; p85; p85-ALPHA; p85alpha; PI3K; PIK3R1
UniProt ID: (Human) P27986, (Mouse) P26450
Entrez Gene ID: (Human) 5295, (Mouse) 18708
transmembrane receptor protein tyrosine kinase adaptor activity
insulin receptor binding
insulin-like growth factor receptor binding
neurotrophin TRKA receptor binding
protein binding
transcription factor binding
1-phosphatidylinositol-3-kinase activity
protein phosphatase binding
phosphatidylinositol 3-kinase regulator activity
phosphatidylinositol 3-kinase regulatory subunit binding
ErbB-3 class receptor binding
phosphatidylinositol 3-kinase binding
insulin binding
insulin receptor substrate binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
1-phosphatidylinositol-3-kinase regulator activity
protein heterodimerization activity
receptor binding
platelet-derived growth factor receptor binding
calmodulin binding
protein C-terminus binding
protein kinase binding
protein domain specific binding
estrogen receptor binding
receptor tyrosine kinase binding
ubiquitin protein ligase binding
ATPase binding
phosphoprotein binding
kinase modulator
cellular glucose homeostasis
negative regulation of cell-matrix adhesion
protein phosphorylation
phosphatidylinositol biosynthetic process
epidermal growth factor receptor signaling pathway
insulin receptor signaling pathway
extrinsic apoptotic signaling pathway via death domain receptors
intrinsic apoptotic signaling pathway in response to DNA damage
phosphatidylinositol 3-kinase signaling
regulation of phosphatidylinositol 3-kinase signaling
viral process
platelet activation
B cell differentiation
positive regulation of cell migration
T cell costimulation
positive regulation of tumor necrosis factor production
cellular response to insulin stimulus
positive regulation of RNA splicing
cellular response to UV
response to endoplasmic reticulum stress
phosphatidylinositol-3-phosphate biosynthetic process
Fc-epsilon receptor signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
ERBB2 signaling pathway
positive regulation of transcription factor import into nucleus
negative regulation of apoptotic process
regulation of phosphatidylinositol 3-kinase activity
negative regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of glucose import
phosphatidylinositol phosphorylation
insulin-like growth factor receptor signaling pathway
vascular endothelial growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
protein stabilization
T cell receptor signaling pathway
leukocyte migration
regulation of stress fiber assembly
NFAT protein import into nucleus
growth hormone receptor signaling pathway
positive regulation of establishment of protein localization to plasma membrane
positive regulation of endoplasmic reticulum unfolded protein response
positive regulation of glucose import in response to insulin stimulus
positive regulation of protein phosphorylation
glucose metabolic process
transport
negative regulation of cell adhesion
signal transduction
negative regulation of heart rate
positive regulation of gene expression
negative regulation of muscle cell apoptotic process
protein transport
negative regulation of cell-cell adhesion
response to insulin
positive regulation of myoblast differentiation
negative regulation of blood pressure
negative regulation of proteolysis
negative regulation of smooth muscle cell proliferation
response to glucocorticoid
response to cAMP
regulation of establishment of protein localization to plasma membrane
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