Invitrogen

PI3K p85 alpha Monoclonal Antibody (U5)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

4 Published Figures
11 References
View all (28) PI3K p85 alpha antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-74183

Applications
Tested Dilution
Publications

Western Blot (WB)

1:100-1:1,000
View 8 publications 8 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent
-

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent
View 1 publication 1 publication

Neutralization (Neu)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Bovine, Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

U5

Immunogen

Recombinant bovine p85 alpha subunit of PI-3 kinase, expressed in Baculovirus.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2163452

Product Specific Information

Mouse anti PI-3 Kinase p85 Subunit alpha antibody, clone U5 recognizes the bcr-homology domain of the p85 alpha subunit of bovine PI 3 kinase.

Target Information

Phosphatidylinositol 3-kinase (PIK3) is a signaling protein that phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. Signaling is initiated when upstream tyrosine kinase receptors or G protein coupled receptors are bound by their ligands. It binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. During insulin stimulation, it also binds to IRS-1. Mutations in the gene can result in Agammaglobulinemia 7, SHORT syndrome, or Immunodeficiency 36.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: p55-GAMMA; phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; Phosphatidylinositol 3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase regulatory subunit gamma; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase-associated p-85 alpha; Phosphoinositide 3-kinase p85 (other splicing variants: p55 and p50); Phosphoinositide 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphoinositide-3-kinase regulatory subunit; phosphoinositide-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha); phosphoinositide-3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); PI3-kinase p85 subunit alpha; PI3-kinase p85-subunit alpha; PI3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; PI3K regulatory subunit gamma; polypeptide 1 p85 alpha; ptdIns-3-kinase p85-alpha; ptdIns-3-kinase regulatory subunit alpha; PtdIns-3-kinase regulatory subunit p85-al; ptdIns-3-kinase regulatory subunit p85-alpha; RP4-533D7.2

View more View less

Gene Aliases: AGM7; GRB1; IMD36; p50alpha; p55alpha; p85; p85-ALPHA; p85alpha; PI3K; PI3KA; PIK3R1

View more View less

UniProt ID: (Human) P27986, (Bovine) P23727, (Mouse) P26450, (Rat) Q63787

View more View less

Entrez Gene ID: (Human) 5295, (Bovine) 282307, (Mouse) 18708, (Rat) 25513

View more View less

Function(s)
transmembrane receptor protein tyrosine kinase adaptor activity insulin receptor binding insulin-like growth factor receptor binding neurotrophin TRKA receptor binding protein binding transcription factor binding 1-phosphatidylinositol-3-kinase activity protein phosphatase binding phosphatidylinositol 3-kinase regulator activity phosphatidylinositol 3-kinase regulatory subunit binding ErbB-3 class receptor binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity 1-phosphatidylinositol-3-kinase regulator activity protein heterodimerization activity phosphotyrosine binding identical protein binding receptor binding platelet-derived growth factor receptor binding calmodulin binding protein C-terminus binding protein kinase binding protein domain specific binding estrogen receptor binding receptor tyrosine kinase binding ubiquitin protein ligase binding ATPase binding phosphoprotein binding kinase modulator
Process(es)
cellular glucose homeostasis negative regulation of cell-matrix adhesion protein phosphorylation phosphatidylinositol biosynthetic process epidermal growth factor receptor signaling pathway insulin receptor signaling pathway extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage phosphatidylinositol 3-kinase signaling regulation of phosphatidylinositol 3-kinase signaling viral process platelet activation B cell differentiation positive regulation of cell migration T cell costimulation positive regulation of tumor necrosis factor production cellular response to insulin stimulus positive regulation of RNA splicing cellular response to UV response to endoplasmic reticulum stress phosphatidylinositol-3-phosphate biosynthetic process Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway positive regulation of transcription factor import into nucleus negative regulation of apoptotic process regulation of phosphatidylinositol 3-kinase activity negative regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import phosphatidylinositol phosphorylation insulin-like growth factor receptor signaling pathway vascular endothelial growth factor receptor signaling pathway phosphatidylinositol-mediated signaling protein stabilization T cell receptor signaling pathway leukocyte migration regulation of stress fiber assembly NFAT protein import into nucleus growth hormone receptor signaling pathway positive regulation of establishment of protein localization to plasma membrane positive regulation of endoplasmic reticulum unfolded protein response positive regulation of glucose import in response to insulin stimulus protein import into nucleus substrate adhesion-dependent cell spreading positive regulation of protein import into nucleus regulation of insulin receptor signaling pathway positive regulation of protein localization to plasma membrane positive regulation of protein phosphorylation glucose metabolic process transport negative regulation of cell adhesion signal transduction negative regulation of heart rate positive regulation of gene expression negative regulation of muscle cell apoptotic process protein transport negative regulation of cell-cell adhesion response to insulin positive regulation of myoblast differentiation negative regulation of blood pressure negative regulation of proteolysis negative regulation of smooth muscle cell proliferation response to glucocorticoid response to cAMP regulation of establishment of protein localization to plasma membrane response to yeast aging response to nutrient response to fructose response to iron(II) ion response to estradiol response to progesterone response to testosterone response to drug response to amino acid response to ethanol response to fatty acid response to growth factor cellular response to fatty acid response to dexamethasone
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support