Invitrogen

PIK3CA Monoclonal Antibody (H.843.0)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

2 Published Figures

3 References

View all (40) PIK3CA antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 5

PIK3CA Antibody (MA5-14870) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of Jurkat (Lane 1), HeLa (Lane 2), U-87 MG (Lane 3), SH-SY5Y (Lane 4) and A-431 (Lane 5). The blot was probed with Anti-PIK3CA Monoclonal Antibody (Product # MA5-14870, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 110 kDa band corresponding to PIK3CA was observed across the cell lines tested.

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Product Details

MA5-14870

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunoprecipitation (IP)

1:50

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

H.843.0

Immunogen

Synthetic peptide surrounding Asp520 of the sequence of human PI3K p110a

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

121 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10984433

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Three-dimensional growth reveals fine-tuning of 5-lipoxygenase by proliferative pathways in cancer.

Life science alliance

Göbel T,Goebel B,Hyprath M,Lamminger I,Weisser H,Angioni C,Mathes M,Thomas D,Kahnt AS

MA5-14870 was used in Western Blotting to show that tumor cells fine-tune 5-LO and LT biosynthesis in response to environmental changes repressing the enzyme during proliferation while making use of the enzyme under cell stress conditions, implying that tumor-derived 5-LO plays a role in the manipulation of the tumor stroma to quickly restore cell proliferation.

Mon May 01 00:00:00 EDT 2023

Angiotensin II receptor blockers valsartan and losartan improve survival rate clinically and suppress tumor growth via apoptosis related to PI3K/AKT signaling in nasopharyngeal carcinoma.

Cancer

Lin YT,Wang HC,Tsai MH,Su YY,Yang MY,Chien CY

MA5-14870 was used in Western Blotting to investigate the survival impacts of ARBs among NPC patients in a retrospective study.

Sat May 15 00:00:00 EDT 2021

Angiotensin II receptor blockers valsartan and losartan improve survival rate clinically and suppress tumor growth via apoptosis related to PI3K/AKT signaling in nasopharyngeal carcinoma.

Cancer

Lin YT,Wang HC,Tsai MH,Su YY,Yang MY,Chien CY

MA5-14870 was used in Western Blotting to investigate the survival impacts of ARBs among NPC patients in a retrospective study.

Sat May 15 00:00:00 EDT 2021

Alterations of the renin angiotensin system in human end-stage heart failure before and after mechanical cardiac unloading by LVAD support.

Molecular and cellular biochemistry

Messmann R,Dietl A,Wagner S,Domenig O,Jungbauer C,Luchner A,Maier LS,Schopka S,Hirt S,Schmid C,Birner C

MA5-14870 was used in Western Blotting to determine the regulation patterns of key components of the RAS and the β-arrestin signaling pathways in left ventricular (LV) tissue.

Tue Sep 01 00:00:00 EDT 2020

Alterations of the renin angiotensin system in human end-stage heart failure before and after mechanical cardiac unloading by LVAD support.

Molecular and cellular biochemistry

Messmann R,Dietl A,Wagner S,Domenig O,Jungbauer C,Luchner A,Maier LS,Schopka S,Hirt S,Schmid C,Birner C

MA5-14870 was used in Western Blotting to determine the regulation patterns of key components of the RAS and the β-arrestin signaling pathways in left ventricular (LV) tissue.

Tue Sep 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: 2.7.1.153; EC:2.7.11.1; GRB1; MGC142161; MGC142163; p110alpha; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform; Phosphoinositide 3-kinase alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; phosphoinositide-3-kinase, catalytic, alpha polypeptide; PI3-kinase p110 subunit alpha; PI3-kinase subunit alpha; PI3K; PI3K alpha; PI3K-alpha; PI3Kalpha; pi3kca; PIK3R1; pk3ca; ptdIns-3-kinase p110; ptdIns-3-kinase subunit alpha; PtdIns-3-kinase subunit p110-alpha; Serine/threonine protein kinase PIK3CA

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Gene Aliases: 6330412C24Rik; caPI3K; CLOVE; CWS5; MCAP; MCM; MCMTC; p110; p110-alpha; p110alpha; PI3K; PI3K-alpha; PIK3CA

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UniProt ID: (Bovine) P32871, (Human) P42336, (Mouse) P42337, (Rat) A0A0G2K344

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Entrez Gene ID: (Bovine) 282306, (Human) 5290, (Mouse) 18706, (Rat) 170911

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Function(s)

protein serine/threonine kinase activity protein binding ATP binding protein kinase activator activity phosphatidylinositol 3-kinase activity insulin receptor substrate binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity phosphatidylinositol-3,4-bisphosphate 5-kinase activity kinase activity 1-phosphatidylinositol-3-kinase activity 1-phosphatidylinositol-4-phosphate 3-kinase activity nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups phosphotransferase activity, alcohol group as acceptor kinase

Process(es)

angiogenesis liver development glucose metabolic process protein phosphorylation phagocytosis regulation of gene expression activation of protein kinase activity positive regulation of peptidyl-serine phosphorylation regulation of multicellular organism growth regulation of cellular respiration protein kinase B signaling negative regulation of neuron apoptotic process hypomethylation of CpG island phosphatidylinositol phosphorylation adipose tissue development cellular response to glucose stimulus energy homeostasis negative regulation of fibroblast apoptotic process regulation of genetic imprinting negative regulation of anoikis vasculature development phosphatidylinositol biosynthetic process epidermal growth factor receptor signaling pathway regulation of phosphatidylinositol 3-kinase signaling platelet activation T cell costimulation phosphatidylinositol-3-phosphate biosynthetic process insulin receptor signaling pathway via phosphatidylinositol 3-kinase Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway endothelial cell migration vascular endothelial growth factor receptor signaling pathway phosphatidylinositol-mediated signaling T cell receptor signaling pathway leukocyte migration cardiac muscle contraction mitophagy in response to mitochondrial depolarization adaptive immune response inflammatory response phosphatidylinositol 3-kinase signaling phosphorylation innate immune response positive regulation of protein kinase activity cell chemotaxis

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PIK3CA Monoclonal Antibody (H.843.0)

PIK3CA Antibody (MA5-14870) in WB

Product Details

MA5-14870

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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