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PIP5K1C Antibody (711474) in ICC/IF
For immunofluorescence analysis, A431 cells were fixed and permeabilized for detection of endogenous Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma using Anti- PIP5K1C Recombinant Rabbit Polyclonal Antibody (Product # 711474, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma protein (green), Pa... View More
Product Details
711474
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
16HCLC
Immunogen
Peptides corresponding to human PIP5K1C (1) aa537-aa551 2) aa330-aa347)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2716900
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Mouse, Bovine
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
PIP5K1C lipid kinase or phosphatidylinositol-4-phosphate 5-kinase 1 gamma phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1C is found at synapses and has been found to play roles in endocytosis and cell migration. PIP5K1C directly associates with -beta-catenin and increases -beta-catenin activity downstream of growth factor stimulation. PIP5K1C expression and kinase activity enhance -beta-catenin phosphorylation on residues that promote nuclear importation and transcriptional activity. PIP5K1C deficiency impairs neutrophil recruitment as a result of defect in adhesion. PIP5K1C regulated the adhesion through facilitation of RhoA GTPase and integrin activation by chemo attractants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: diphosphoinositide kinase; Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma; phosphatidylinositol-4-phosphate 5-kinase; phosphatidylinositol-4-phosphate 5-kinase, type I, gamma; PIP5K1gamma; PIP5KI gamma; PIP5KI-gamma; PIPKIg_v4; ptdIns(4)P-5-kinase 1 gamma; Type I phosphatidylinositol 4-phosphate 5-kinase gamma; type I PIP kinase
Gene Aliases: KIAA0589; LCCS3; PIP5K-GAMMA; PIP5K1-gamma; PIP5K1C; PIP5Kgamma
UniProt ID: (Human) O60331
Entrez Gene ID: (Human) 23396
phosphatidylinositol biosynthetic process
phagocytosis
regulation of phosphatidylinositol 3-kinase signaling
synaptic vesicle exocytosis
single organismal cell-cell adhesion
actin cytoskeleton organization
neutrophil chemotaxis
adherens junction assembly
phosphatidylinositol phosphorylation
synaptic vesicle endocytosis
clathrin-mediated endocytosis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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