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PKC alpha Polyclonal Antibody

PKC alpha Antibody in Immunocytochemistry (ICC/IF)

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PKC alpha Antibody (21991-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Alpha antibody (Product # 21991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

PKC alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PKC alpha Antibody in Flow Cytometry (Flow)

PKC alpha Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

21991-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 31 publications 31 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 8 publications 8 publications

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PKC alpha Fusion Protein Ag17275 (1-216 aa encoded by AK055431)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.36 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MADVFPGND STASQDVANR FARKGALRQK NVHEVKDHKF IARFFKQPTF CSHCTDFIWG FGKQGFQCQV CCFVVHKRCH EFVTFSCPGA DKGPDTDDPR SKHKFKIHTY GSPTFCDHCG SLLYGLIHQG MKCDTCDMNV HKQCVINVPS LCGMDHTEKR GRIYLKAEVA DEKLHVTVRD AKNLIPMDPN GLSDPYVKLK LIPDPKNESK QKTKTIR (1-216 aa encoded by AK055431)

Target Information

The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: aging-associated gene 6; EC 2.7.11.13; FHC; FTH; FTHL6; kinase PKC-alpha; KPCA; MGC104426; PIG15; PKC III; PKC-A; PKC-alpha; PKC-III; PLIF; Protein kinase C alpha type; protein kinase C, alpha; protein kinase c-alpha

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Gene Aliases: AAG6; AI875142; PKC-alpha; PKCA; PRKACA; PRKCA

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UniProt ID: (Human) P17252, (Mouse) P20444

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Entrez Gene ID: (Human) 5578, (Mouse) 18750, (Rat) 24680

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Function(s)

protein kinase activity protein serine/threonine kinase activity protein kinase C activity calcium-dependent protein kinase C activity protein binding ATP binding zinc ion binding enzyme binding histone kinase activity (H3-T6 specific) nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding non-receptor serine/threonine protein kinase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PKC alpha Polyclonal Antibody

PKC alpha Antibody (21991-1-AP) in ICC/IF

Product Details

21991-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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