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PKC gamma Polyclonal Antibody

PKC gamma Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

PKC gamma Antibody (14364-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using 14364-1-AP (PKC gamma antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

PKC gamma Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PKC gamma Antibody in Flow Cytometry (Flow)

PKC gamma Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14364-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:20,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 2 publications 2 publications

Flow Cytometry (Flow)

1 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Mouse, Rat, Human

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PKC gamma Fusion Protein Ag5681 (351-697 aa encoded by BC047876)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: FSFLMVLGK GSFGKVMLAE RRGSDELYAI KILKKDVIVQ DDDVDCTLVE KRVLALGGRG PGGRPHFLTQ LHSTFQTPDR LYFVMEYVTG GDLMYHIQQL GKFKEPHAAF YAAEIAIGLF FLHNQGIIYR DLKLDNVMLD AEGHIKITDF GMCKENVFPG TTTRTFCGTP DYIAPEIIAY QPYGKSVDWW SFGVLLYEML AGQPPFDGED EEELFQAIME QTVTYPKSLS REAVAICKGF LTKHPGKRLG SGPDGEPTIR AHGFFRWIDW ERLERLEIPP PFRPRPCGRS GENFDKFFTR AAPALTPPDR LVLASIDQAD FQGFTYVNPD FVHPDARSPT SPVPVPVM (351-697 aa encoded by BC047876)

Target Information

The PKC family of serine/threonine kinases, including PRKCG (PKC gamma), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC gamma is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The SLITRK4-CNPY3 axis promotes liver metastasis of gastric cancer by enhancing the endocytosis and recycling of TrkB in tumour cells.

Cellular oncology (Dordrecht)

Zhou YQ,Bao TS,Xie JX,Yao LL,Yu ST,Li Q,Huang PQ,Zhou WZ,Wang YY,Chen SY,Wang XQ,Zhang XL,Jiang SH,Yi SQ,Zhang ZG,Ma MZ,Hu LP,Xu J,Li J

Tue Aug 01 00:00:00 EDT 2023

Mouse model of voluntary movement deficits induced by needlestick injuries to the primary motor cortex.

Journal of neuroscience methods

Liu XT,Liu CM,Teng ZQ

Sat Jan 01 00:00:00 EST 2022

Maternal urban particulate matter exposure and signaling pathways in fetal brains and neurobehavioral development in offspring.

Toxicology

Li S,Liu Y,Liu B,Hu YQ,Ding YQ,Zhang J,Feng L

Mon May 30 00:00:00 EDT 2022

PKC signal amplification suppresses non-small cell lung cancer growth by promoting p21 expression and phosphorylation.

Heliyon

Liu S,Zhang Y,Yang Q,Zhang Y,Liu H,Huang MH,Wang R,Lu F

Thu Sep 01 00:00:00 EDT 2022

Delta Opioid Receptor Activation with Delta Opioid Peptide [d-Ala2, d-Leu5] Enkephalin Contributes to Synaptic Improvement in Rat Hippocampus against Global Ischemia.

Cell transplantation

Zhang G,Lai Z,Gu L,Xu K,Wang Z,Duan Y,Chen H,Zhang M,Zhang J,Zhao Z,Wang S

Tue Dec 07 00:00:00 EST 2021

LRRC19 Promotes Permeability of the Gut Epithelial Barrier Through Degrading PKC-ζ and PKCι/λ to Reduce Expression of ZO1, ZO3, and Occludin.

Inflammatory bowel diseases

Su X,Wei J,Qi H,Jin M,Zhang Q,Zhang Y,Zhang C,Yang R

Tue Jul 27 00:00:00 EDT 2021

LRRC19 Promotes Permeability of the Gut Epithelial Barrier Through Degrading PKC-ζ and PKCι/λ to Reduce Expression of ZO1, ZO3, and Occludin.

Inflammatory bowel diseases

Su X,Wei J,Qi H,Jin M,Zhang Q,Zhang Y,Zhang C,Yang R

Tue Jul 27 00:00:00 EDT 2021

Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.

Science advances

Antico O,Ordureau A,Stevens M,Singh F,Nirujogi RS,Gierlinski M,Barini E,Rickwood ML,Prescott A,Toth R,Ganley IG,Harper JW,Muqit MMK

Fri Nov 12 00:00:00 EST 2021

Dendritic Spine Dynamics after Peripheral Nerve Injury: An Intravital Structural Study.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Benson CA,Fenrich KK,Olson KL,Patwa S,Bangalore L,Waxman SG,Tan AM

Wed May 27 00:00:00 EDT 2020

Perinatal exposure to nonylphenol impairs dendritic outgrowth of cerebellar Purkinje cells in progeny.

Chemosphere

You M,Gu W,Li M,Qiu Z,Li S,Jiang Z,Yao D,Xu Y,Wang Y

Thu Nov 01 00:00:00 EDT 2018

Perinatal exposure to nonylphenol impairs dendritic outgrowth of cerebellar Purkinje cells in progeny.

Chemosphere

You M,Gu W,Li M,Qiu Z,Li S,Jiang Z,Yao D,Xu Y,Wang Y

Thu Nov 01 00:00:00 EDT 2018

Analgesic effects and possible mechanisms of iridoid glycosides from Lamiophlomis rotata (Benth.) Kudo in rats with spared nerve injury.

Journal of ethnopharmacology

Zheng Y,Yin X,Huo F,Xiong H,Mei Z

Tue Sep 15 00:00:00 EDT 2015

Analgesic effects and possible mechanisms of iridoid glycosides from Lamiophlomis rotata (Benth.) Kudo in rats with spared nerve injury.

Journal of ethnopharmacology

Zheng Y,Yin X,Huo F,Xiong H,Mei Z

Tue Sep 15 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: PKC-gamma; Protein kinase C gamma type; protein kinase C type I (gamma type); protein kinase C, gamma

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Gene Aliases: PKC; PKC-gamma; PKCC; PKCG; PKCgamma; PKCI; Prkc; Prkcc; PRKCG; RATPKCI; SCA14

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UniProt ID: (Human) P05129, (Mouse) P63318, (Rat) P63319

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Entrez Gene ID: (Human) 5582, (Mouse) 18752, (Rat) 24681

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Function(s)

protein kinase activity protein kinase C activity calcium-dependent protein kinase C activity protein serine/threonine/tyrosine kinase activity ATP binding zinc ion binding nucleotide binding protein serine/threonine kinase activity protein binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding non-receptor serine/threonine protein kinase

Process(es)

protein phosphorylation chemical synaptic transmission learning or memory chemosensory behavior phosphorylation peptidyl-serine phosphorylation platelet activation negative regulation of protein ubiquitination regulation of response to food positive regulation of mismatch repair intracellular signal transduction negative regulation of protein catabolic process regulation of circadian rhythm response to morphine negative regulation of neuron apoptotic process protein autophosphorylation response to pain rhythmic process regulation of phagocytosis innervation negative regulation of proteasomal protein catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PKC gamma Polyclonal Antibody

PKC gamma Antibody (14364-1-AP) in ICC/IF

Product Details

14364-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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