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FIGURE: 1 / 1
PKC gamma Antibody (PA5-86906) in WB
Western blot analysis of PKC gamma in Lane 1: A549 whole cell lysate (40 µg), Lane 2: brain tissue lysate of rat (40 µg), Lane 3: Placenta tissue lysate of mouse (40 µg). Sample was incubated with PKC gamma polyclonal antibody (Product # PA5-86906) using a 1:500 dilution.
Product Details
PA5-86906
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide, corresponding to amino acids 543-587 of Human PRKCG
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2803664
Product Specific Information
Purity is > 95% by SDS-PAGE.
Target Information
The PKC family of serine/threonine kinases, including PRKCG (PKC gamma), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC gamma is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PKC-gamma; Protein kinase C gamma type; protein kinase C type I (gamma type); protein kinase C, gamma
Gene Aliases: PKC; PKC-gamma; PKCC; PKCG; PKCgamma; PKCI; Prkc; Prkcc; PRKCG; RATPKCI; SCA14
UniProt ID: (Human) P05129, (Mouse) P63318, (Rat) P63319
Entrez Gene ID: (Human) 5582, (Mouse) 18752, (Rat) 24681
protein kinase activity
protein kinase C activity
calcium-dependent protein kinase C activity
protein serine/threonine/tyrosine kinase activity
ATP binding
zinc ion binding
nucleotide binding
protein serine/threonine kinase activity
protein binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
non-receptor serine/threonine protein kinase
protein phosphorylation
chemical synaptic transmission
learning or memory
chemosensory behavior
phosphorylation
peptidyl-serine phosphorylation
platelet activation
negative regulation of protein ubiquitination
regulation of response to food
positive regulation of mismatch repair
intracellular signal transduction
negative regulation of protein catabolic process
regulation of circadian rhythm
response to morphine
negative regulation of neuron apoptotic process
protein autophosphorylation
response to pain
rhythmic process
regulation of phagocytosis
innervation
negative regulation of proteasomal protein catabolic process
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