Invitrogen

PKC gamma Monoclonal Antibody (PKC66)

6 Published Figures

17 References

View all (25) PKC gamma antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (6)

PKC gamma Antibody in Immunohistochemistry (Frozen) (IHC (F))

FIGURE: 1 / 6

PKC gamma Antibody (13-3800) in IHC (F)

Molecular pain 2012 - Figure 4 Dorsal root rhizotomy causes a dramatic loss of Pap and Cgrp but significantly smaller changes of bombesin and Pkcgamma in dSC. L4, L5, and L6 central roots of two month old wild type mice were transected, and mice were examined two weeks following surgery. Sections of the lumbar enlargement were immunostained to examine the loss of proteins and peptides caused by dorsal root axonal degeneration an... View More

PKC gamma Antibody in Immunohistochemistry (IHC)

PKC gamma Antibody in Proximity Ligation Assay (PLA) (PLA)

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Product Details

13-3800

Applications

Tested Dilution

Publications

Western Blot (WB)

2-5 µg/mL

View 7 publications 7 publications

Immunohistochemistry (IHC)

Assay-dependent

View 6 publications 6 publications

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

in situ PLA (PLA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Guinea pig, Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

PKC66

Immunogen

Purified rat brain PKC.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533015

Target Information

The PKC family of serine/threonine kinases, including PRKCG (PKC gamma), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC gamma is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PKCγ-Mediated Phosphorylation of CRMP2 Regulates Dendritic Outgrowth in Cerebellar Purkinje Cells.

Molecular neurobiology

Winkler SC,Shimobayashi E,Kapfhammer JP

13-3800 was used in Proximity Ligation Assay (PLA) to show that Vangl2 exerts a bimodal regulation on young and mature GCs, and its disruption leads to an imbalance in hippocampus-dependent pattern completion and separation processes.

Tue Dec 01 00:00:00 EST 2020

PKCγ-Mediated Phosphorylation of CRMP2 Regulates Dendritic Outgrowth in Cerebellar Purkinje Cells.

Molecular neurobiology

Winkler SC,Shimobayashi E,Kapfhammer JP

Tue Dec 01 00:00:00 EST 2020

RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma.

Cancer cell

Chen X,Wu Q,Depeille P,Chen P,Thornton S,Kalirai H,Coupland SE,Roose JP,Bastian BC

13-3800 was used in Western Blotting to show that RasGRP3 is a therapeutic target for cancers driven by oncogenic GNAQ/11.

Mon May 08 00:00:00 EDT 2017

Cancer-associated protein kinase C mutations reveal kinase's role as tumor suppressor.

Cell

Antal CE,Hudson AM,Kang E,Zanca C,Wirth C,Stephenson NL,Trotter EW,Gallegos LL,Miller CJ,Furnari FB,Hunter T,Brognard J,Newton AC

Published figure using PKC gamma monoclonal antibody (Product # 13-3800) in Western Blot

Thu Jan 29 00:00:00 EST 2015

Hint1 knockout results in a compromised activation of protein kinase C gamma in the brain.

Brain research

Zhang F,Fang Z,Wang JB

13-3800 was used in western blot to investigate the role of in the function of brain protein kinase C gamma

Mon Oct 05 00:00:00 EDT 2015

Paraneoplastic CDR2 and CDR2L antibodies affect Purkinje cell calcium homeostasis.

Acta neuropathologica

Schubert M,Panja D,Haugen M,Bramham CR,Vedeler CA

13-3800 was used in western blot to investigate the effect of CDR2 and CDR2L antibodies on Purkinje cell calcium homeostasis.

Mon Dec 01 00:00:00 EST 2014

Distribution of gastrin-releasing peptide in the rat trigeminal and spinal somatosensory systems.

The Journal of comparative neurology

Takanami K,Sakamoto H,Matsuda KI,Satoh K,Tanida T,Yamada S,Inoue K,Oti T,Sakamoto T,Kawata M

Sun Jun 01 00:00:00 EDT 2014

Dynamic genotype-selective "phenotypic switching" of CGRP expression contributes to differential neuropathic pain phenotype.

Experimental neurology

Nitzan-Luques A,Minert A,Devor M,Tal M

13-3800 was used in immunohistochemistry - frozen section to test if calcitonin gene related peptide is the principal driver of tactile allodynia.

Sun Dec 01 00:00:00 EST 2013

The majority of dorsal spinal cord gastrin releasing peptide is synthesized locally whereas neuromedin B is highly expressed in pain- and itch-sensing somatosensory neurons.

Molecular pain

Fleming MS,Ramos D,Han SB,Zhao J,Son YJ,Luo W

13-3800 was used in immunohistochemistry - frozen section to examine the expression of Grp and Nmb.

Mon Jul 09 00:00:00 EDT 2012

Genotype-selective phenotypic switch in primary afferent neurons contributes to neuropathic pain.

Pain

Nitzan-Luques A,Devor M,Tal M

13-3800 was used in immunohistochemistry to test if phenotypic switching in neurotransmitters expressed by Aβ afferents account for heritable differences in neuropathic pain behavior.

Sat Oct 01 00:00:00 EDT 2011

Involvement of Ca2+-dependent PKCs in the adaptive changes of mu-opioid pathways to sympathetic denervation in the guinea pig colon.

Biochemical pharmacology

Giaroni C,Zanetti E,Pascale A,Oldrini R,Canciani L,Giuliani D,Amadio M,Chiaravalli AM,Lecchini S,Frigo GM

Sun Nov 01 00:00:00 EDT 2009

Involvement of Ca2+-dependent PKCs in the adaptive changes of mu-opioid pathways to sympathetic denervation in the guinea pig colon.

Biochemical pharmacology

Giaroni C,Zanetti E,Pascale A,Oldrini R,Canciani L,Giuliani D,Amadio M,Chiaravalli AM,Lecchini S,Frigo GM

Sun Nov 01 00:00:00 EDT 2009

Expression of spinal NMDA receptor and PKCgamma after chronic morphine is regulated by spinal glucocorticoid receptor.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Lim G,Wang S,Zeng Q,Sung B,Yang L,Mao J

Wed Nov 30 00:00:00 EST 2005

Protein kinase C betaII plays an essential role in dendritic cell differentiation and autoregulates its own expression.

The Journal of biological chemistry

Cejas PJ,Carlson LM,Zhang J,Padmanabhan S,Kolonias D,Lindner I,Haley S,Boise LH,Lee KP

Fri Aug 05 00:00:00 EDT 2005

Amyloid plaques in cerebellar cortex and the integrity of Purkinje cell dendrites.

Neurobiology of aging

Li YT,Woodruff-Pak DS,Trojanowski JQ

Fri May 13 00:00:00 EDT 1994

Neurons derived from a human teratocarcinoma cell line establish molecular and structural polarity following transplantation into the rodent brain.

Experimental neurology

Trojanowski JQ,Mantione JR,Lee JH,Seid DP,You T,Inge LJ,Lee VM

Sun Aug 01 00:00:00 EDT 1993

Neurons derived from a human teratocarcinoma cell line establish molecular and structural polarity following transplantation into the rodent brain.

Experimental neurology

Trojanowski JQ,Mantione JR,Lee JH,Seid DP,You T,Inge LJ,Lee VM

Sun Aug 01 00:00:00 EDT 1993

Characterization and differential distribution of the three major human protein kinase C isozymes (PKC alpha, PKC beta, and PKC gamma) of the central nervous system in normal and Alzheimer's disease brains.

Laboratory investigation; a journal of technical methods and pathology

Clark EA,Leach KL,Trojanowski JQ,Lee VM

Tue Jan 01 00:00:00 EST 1991

Laboratory investigation; a journal of technical methods and pathology

Clark EA,Leach KL,Trojanowski JQ,Lee VM

Tue Jan 01 00:00:00 EST 1991

Human olfactory epithelium in normal aging, Alzheimer's disease, and other neurodegenerative disorders.

The Journal of comparative neurology

Trojanowski JQ,Newman PD,Hill WD,Lee VM

Thu Aug 15 00:00:00 EDT 1991

Bioinformatics

Protein Aliases: PKC-gamma; Protein kinase C gamma type; protein kinase C type I (gamma type); protein kinase C, gamma

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Gene Aliases: PKC; PKC-gamma; PKCC; PKCG; PKCgamma; PKCI; Prkc; Prkcc; PRKCG; RATPKCI; SCA14

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UniProt ID: (Human) P05129, (Mouse) P63318, (Rat) P63319

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Entrez Gene ID: (Human) 5582, (Mouse) 18752, (Rat) 24681

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Function(s)

protein kinase activity protein kinase C activity calcium-dependent protein kinase C activity protein serine/threonine/tyrosine kinase activity ATP binding zinc ion binding nucleotide binding protein serine/threonine kinase activity protein binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding non-receptor serine/threonine protein kinase

Process(es)

protein phosphorylation chemical synaptic transmission learning or memory chemosensory behavior phosphorylation peptidyl-serine phosphorylation platelet activation negative regulation of protein ubiquitination regulation of response to food positive regulation of mismatch repair intracellular signal transduction negative regulation of protein catabolic process regulation of circadian rhythm response to morphine negative regulation of neuron apoptotic process protein autophosphorylation response to pain rhythmic process regulation of phagocytosis innervation negative regulation of proteasomal protein catabolic process

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PKC gamma Monoclonal Antibody (PKC66)

PKC gamma Antibody (13-3800) in IHC (F)

Product Details

13-3800

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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