Invitrogen

PKP3 Monoclonal Antibody (23E3/4)

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

10 Published Figures

15 References

View all (13) PKP3 antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 13

PKP3 Antibody (35-7600) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-431 (Lane 1), A549 (Lane 2), HEK-293 (Lane 3), SK-OV-3 (Lane 4) and COLO 205 (Lane 5). The blots were probed with Mouse Anti-PKP3 Monoclonal Antibody (Product # 35-7600, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 87 kDa band corresponding to PKP3 was observed across the cell lines tested. Known quantity of protein samples were electrop... View More

PKP3 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

PKP3 Antibody in Immunocytochemistry (ICC/IF)

PKP3 Antibody in Immunohistochemistry (IHC)

PKP3 Antibody in Western Blot, ChIP Assay (WB, ChIP)

Product Image Gallery

PKP3 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Plakophilin-3 siRNA and loss of signal was observed in Western Blot using Anti-Plakophilin-3 Antibody (Product # 35-7600). {KD}

Product Details

35-7600

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

Assay-dependent

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

ChIP assay (ChIP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

23E3/4

Immunogen

Synthetic peptide from Armadillo domain of human Plakophilin-3.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533221

Target Information

Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalization, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localization in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of ~87 kDa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Disruption of desmosome function leads to increased centrosome clustering in 14-3-3γ-knockout cells with supernumerary centrosomes.

FEBS letters

Tilwani S,Gandhi K,Narayan S,Ainavarapu SRK,Dalal SN

Published figure using PKP3 monoclonal antibody (Product # 35-7600) in Immunocytochemistry

Mon Nov 01 00:00:00 EDT 2021

Disruption of desmosome function leads to increased centrosome clustering in 14-3-3γ-knockout cells with supernumerary centrosomes.

FEBS letters

Tilwani S,Gandhi K,Narayan S,Ainavarapu SRK,Dalal SN

Published figure using PKP3 monoclonal antibody (Product # 35-7600) in Immunocytochemistry

Mon Nov 01 00:00:00 EDT 2021

Proteomic characterization of GSK3β knockout shows altered cell adhesion and metabolic pathway utilisation in colorectal cancer cells.

PloS one

Bowler-Barnett E,Martinez-Garcia FD,Sherwood M,Aleidan A,John S,Weston S,Wang Y,Divecha N,Skipp P,Ewing RM

Published figure using PKP3 monoclonal antibody (Product # 35-7600) in Western Blot

Wed Dec 29 00:00:00 EST 2021

Proteomic characterization of GSK3β knockout shows altered cell adhesion and metabolic pathway utilisation in colorectal cancer cells.

PloS one

Bowler-Barnett E,Martinez-Garcia FD,Sherwood M,Aleidan A,John S,Weston S,Wang Y,Divecha N,Skipp P,Ewing RM

Published figure using PKP3 monoclonal antibody (Product # 35-7600) in Western Blot

Wed Dec 29 00:00:00 EST 2021

Increased Cholesterol Biosynthesis Is a Key Characteristic of Breast Cancer Stem Cells Influencing Patient Outcome.

Cell reports

Ehmsen S,Pedersen MH,Wang G,Terp MG,Arslanagic A,Hood BL,Conrads TP,Leth-Larsen R,Ditzel HJ

35-7600 was used in Immunohistochemistry to identify the cholesterol biosynthesis pathway as central for CSC propagation and a potential therapeutic target, as well as providing a mechanistic explanation for the therapeutic benefit of statins in breast cancer.

Tue Jun 25 00:00:00 EDT 2019

MMP7 is required to mediate cell invasion and tumor formation upon Plakophilin3 loss.

PloS one

Basu S,Thorat R,Dalal SN

Published figure using PKP3 monoclonal antibody (Product # 35-7600) in Western Blot

Wed Jan 13 00:00:00 EST 2016

Plakophilin3 loss leads to an increase in PRL3 levels promoting K8 dephosphorylation, which is required for transformation and metastasis.

PloS one

Khapare N,Kundu ST,Sehgal L,Sawant M,Priya R,Gosavi P,Gupta N,Alam H,Karkhanis M,Naik N,Vaidya MM,Dalal SN

Published figure using PKP3 monoclonal antibody (Product # 35-7600) in Immunofluorescence

Thu Nov 08 00:00:00 EST 2012

Plakophilin3 loss leads to an increase in PRL3 levels promoting K8 dephosphorylation, which is required for transformation and metastasis.

PloS one

Khapare N,Kundu ST,Sehgal L,Sawant M,Priya R,Gosavi P,Gupta N,Alam H,Karkhanis M,Naik N,Vaidya MM,Dalal SN

Published figure using PKP3 monoclonal antibody (Product # 35-7600) in Immunofluorescence

Thu Nov 08 00:00:00 EST 2012

Unimpaired skin carcinogenesis in Desmoglein 3 knockout mice.

PloS one

Baron S,Hoang A,Vogel H,Attardi LD

35-7600 was used in immunocytochemistry to explore the role of desmosomal cadherins in carcinogenesis.

Thu May 30 00:00:00 EDT 2013

E-cadherin and plakoglobin recruit plakophilin3 to the cell border to initiate desmosome assembly.

Cellular and molecular life sciences : CMLS

Gosavi P,Kundu ST,Khapare N,Sehgal L,Karkhanis MS,Dalal SN

35-7600 was used in immunocytochemistry and western blot to test if plakophilin3 is required for desmosome formation

Fri Apr 01 00:00:00 EDT 2011

The transcription factor ZEB1 (deltaEF1) represses Plakophilin 3 during human cancer progression.

FEBS letters

Aigner K,Descovich L,Mikula M,Sultan A,Dampier B,Bonné S,van Roy F,Mikulits W,Schreiber M,Brabletz T,Sommergruber W,Schweifer N,Wernitznig A,Beug H,Foisner R,Eger A

Published figure using PKP3 monoclonal antibody (Product # 35-7600) in Immunofluorescence

Tue Apr 17 00:00:00 EDT 2007

The transcription factor ZEB1 (deltaEF1) represses Plakophilin 3 during human cancer progression.

FEBS letters

Aigner K,Descovich L,Mikula M,Sultan A,Dampier B,Bonné S,van Roy F,Mikulits W,Schreiber M,Brabletz T,Sommergruber W,Schweifer N,Wernitznig A,Beug H,Foisner R,Eger A

Published figure using PKP3 monoclonal antibody (Product # 35-7600) in Immunofluorescence

Tue Apr 17 00:00:00 EDT 2007

The transcription factor ZEB1 (deltaEF1) represses Plakophilin 3 during human cancer progression.

FEBS letters

Aigner K,Descovich L,Mikula M,Sultan A,Dampier B,Bonné S,van Roy F,Mikulits W,Schreiber M,Brabletz T,Sommergruber W,Schweifer N,Wernitznig A,Beug H,Foisner R,Eger A

Published figure using PKP3 monoclonal antibody (Product # 35-7600) in Immunofluorescence

Tue Apr 17 00:00:00 EDT 2007

The transcription factor ZEB1 (deltaEF1) represses Plakophilin 3 during human cancer progression.

FEBS letters

Aigner K,Descovich L,Mikula M,Sultan A,Dampier B,Bonné S,van Roy F,Mikulits W,Schreiber M,Brabletz T,Sommergruber W,Schweifer N,Wernitznig A,Beug H,Foisner R,Eger A

Published figure using PKP3 monoclonal antibody (Product # 35-7600) in Immunofluorescence

Tue Apr 17 00:00:00 EDT 2007

Plakophilin 3 oncogene as prognostic marker and therapeutic target for lung cancer.

Cancer research

Furukawa C,Daigo Y,Ishikawa N,Kato T,Ito T,Tsuchiya E,Sone S,Nakamura Y

Mon Aug 15 00:00:00 EDT 2005

Plakophilin 3 oncogene as prognostic marker and therapeutic target for lung cancer.

Cancer research

Furukawa C,Daigo Y,Ishikawa N,Kato T,Ito T,Tsuchiya E,Sone S,Nakamura Y

Mon Aug 15 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: plakophilin 3b; Plakophilin-3

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Gene Aliases: PKP3

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UniProt ID: (Human) Q9Y446

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Entrez Gene ID: (Human) 11187

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Function(s)

alpha-catenin binding cell adhesion molecule binding cadherin binding involved in cell-cell adhesion intermediate filament binding protein

Process(es)

desmosome assembly single organismal cell-cell adhesion establishment of protein localization to plasma membrane

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PKP3 Monoclonal Antibody (23E3/4)

PKP3 Antibody (35-7600) in WB

Product Details

35-7600

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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