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PLA2G4A Antibody (PA5-19324) in ICC/IF
Immunocytochemistry analysis of PLA2G4A using PLA2G4A Polyclonal Antibody (Product # PA5-19324) in paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-19324
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (QEKTFRQQRKEHIR) corresponding to the internal amino acids of PLA2G4A
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_10981642
Product Specific Information
This antibody is predicted to react with rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: calcium-dependent phospholipid-binding protein; cPLA2; cytosolic group IVA; Cytosolic phospholipase A2; group IVA; lysophospholipase; MGC126350; phosphatidylcholine 2-acylhydrolase; Phospholipase A2 group IVA; phospholipase A2, group IVA (cytosolic, calcium-dependent)
Gene Aliases: CPLA2; cPLA2-alpha; PLA2G4; PLA2G4A
UniProt ID: (Human) P47712
Entrez Gene ID: (Human) 5321
ovulation from ovarian follicle
luteolysis
phospholipid metabolic process
phosphatidic acid biosynthetic process
platelet activating factor biosynthetic process
icosanoid metabolic process
aging
positive regulation of cell proliferation
phospholipid catabolic process
response to heat
response to lithium ion
response to organonitrogen compound
arachidonic acid metabolic process
positive regulation of bone mineralization
positive regulation of vesicle fusion
positive regulation of prostaglandin biosynthetic process
positive regulation of fever generation
response to lipopolysaccharide
response to vitamin D
cardiolipin acyl-chain remodeling
phosphatidylglycerol acyl-chain remodeling
phosphatidylinositol acyl-chain remodeling
phosphatidylserine acyl-chain remodeling
phosphatidylcholine acyl-chain remodeling
phosphatidylethanolamine acyl-chain remodeling
response to hydrogen peroxide
positive regulation of apoptotic process
surfactant homeostasis
icosanoid biosynthetic process
decidualization
arachidonic acid secretion
response to glucocorticoid
response to calcium ion
response to methylmercury
cellular response to antibiotic
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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