Proteintech

PLK1 Polyclonal Antibody

PLK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PLK1 Antibody (10305-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue slide using (Product # 10305-1-AP) PLK1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Product Image Gallery

Product Details

10305-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PLK1 Fusion Protein Ag0284 (415-603 aa encoded by BC002369)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.16 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: VDYSDKYGL GYQLCDNSVG VLFNDSTRLI LYNDGDSLQY IERDGTESYL TVSSHPNSLM KKITLLKYFR NYMSEHLLKA GANITPREGD ELARLPYLRT WFRTRSAIIL HLSNGSVQIN FFQDHTKLIL CPLMAAVTYI DEKRDFRTYR LSLLEEYGCC KELASRLRYA RTMVDKLLSS RSASNRLKAS (415-603 aa encoded by BC002369)

Target Information

PLK1 is a serine/threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

USP1 promotes the aerobic glycolysis and progression of T-cell acute lymphoblastic leukemia via PLK1/LDHA axis.

Blood advances

Liu S,Xiang Y,Wang B,Gao C,Chen Z,Xie S,Wu J,Liu Y,Zhao X,Yang C,Yue Z,Wang L,Wen X,Zhang R,Zhang F,Xu H,Zhai X,Zheng H,Zhang H,Qian M

Tue Jul 11 00:00:00 EDT 2023

USP1 promotes the aerobic glycolysis and progression of T-cell acute lymphoblastic leukemia via PLK1/LDHA axis.

Blood advances

Liu S,Xiang Y,Wang B,Gao C,Chen Z,Xie S,Wu J,Liu Y,Zhao X,Yang C,Yue Z,Wang L,Wen X,Zhang R,Zhang F,Xu H,Zhai X,Zheng H,Zhang H,Qian M

Tue Jul 11 00:00:00 EDT 2023

Tectoridin and PLK1 inhibitor synergistically promote the apoptosis of lung adenocarcinoma cells: Bioinformatic analysis of TCGA and TCMSP.

Naunyn-Schmiedeberg's archives of pharmacology

Wang M,Zhao F,Li Z,Li X,Dong L

Sun Oct 01 00:00:00 EDT 2023

Comprehensive Analysis of Necroptosis Landscape in Skin Cutaneous Melanoma for Appealing its Implications in Prognosis Estimation and Microenvironment Status.

Journal of personalized medicine

Cao X,He J,Chen A,Ran J,Li J,Chen D,Zhang H

Sun Jan 29 00:00:00 EST 2023

Parasympathetic-macrophages-ductal epithelial cells axis promotes female rat submandibular gland regeneration after excretory duct ligation/deligation.

Archives of oral biology

Wang B,Li D,Li J,An W,Li Z,Shao Q,Qin L

Sun Jan 01 00:00:00 EST 2023

Pan-Cancer Transcriptomic Analysis Identifies PLK1 Crucial for the Tumorigenesis of Clear Cell Renal Cell Carcinoma.

Journal of inflammation research

Qian Y,Li Y,Chen K,Liu N,Hong X,Wu D,Xu Z,Zhou L,Xu L,Jia R,Ge YZ

Mon May 02 00:00:00 EDT 2022

Vangl2 participates in the primary ciliary assembly under low fluid shear stress in hUVECs.

Cell and tissue research

Sheng X,Gao S,Sheng Y,Xie X,Wang J,He Y

Sat Jan 01 00:00:00 EST 2022

Vangl2 participates in the primary ciliary assembly under low fluid shear stress in hUVECs.

Cell and tissue research

Sheng X,Gao S,Sheng Y,Xie X,Wang J,He Y

Sat Jan 01 00:00:00 EST 2022

Oxcarbazepine induces mitotic catastrophe and apoptosis in NRK-52E proximal tubular cells.

Toxicology letters

Ota M,Funakoshi T,Aki T,Unuma K,Uemura K

Sun Oct 10 00:00:00 EDT 2021

Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer.

Nature communications

Shi W,Zhang G,Ma Z,Li L,Liu M,Qin L,Yu Z,Zhao L,Liu Y,Zhang X,Qin J,Ye H,Jiang X,Zhou H,Sun H,Jiao Z

Fri May 14 00:00:00 EDT 2021

Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer.

Nature communications

Shi W,Zhang G,Ma Z,Li L,Liu M,Qin L,Yu Z,Zhao L,Liu Y,Zhang X,Qin J,Ye H,Jiang X,Zhou H,Sun H,Jiao Z

Fri May 14 00:00:00 EDT 2021

Stimuli-Responsive and Highly Penetrable Nanoparticles as a Multifunctional Nanoplatform for Boosting Nonsmall Cell Lung Cancer siRNA Therapy.

ACS biomaterials science & engineering

Shi M,Wang Y,Zhao X,Zhang J,Hu H,Qiao M,Zhao X,Chen D

Mon Jul 12 00:00:00 EDT 2021

Stimuli-Responsive and Highly Penetrable Nanoparticles as a Multifunctional Nanoplatform for Boosting Nonsmall Cell Lung Cancer siRNA Therapy.

ACS biomaterials science & engineering

Shi M,Wang Y,Zhao X,Zhang J,Hu H,Qiao M,Zhao X,Chen D

Mon Jul 12 00:00:00 EDT 2021

PLK1 Inhibition alleviates transplant-associated obliterative bronchiolitis by suppressing myofibroblast differentiation.

Aging

Yu J,Xu H,Cui J,Chen S,Zhang H,Zou Y,Zhao J,Le S,Jiang L,Chen Z,Liu H,Zhang D,Xia J,Wu J

Mon Jun 15 00:00:00 EDT 2020

CDK1-PLK1/SGOL2/ANLN pathway mediating abnormal cell division in cell cycle may be a critical process in hepatocellular carcinoma.

Cell cycle (Georgetown, Tex.)

Li L,Huang K,Zhao H,Chen B,Ye Q,Yue J

Fri May 01 00:00:00 EDT 2020

Translation control of the immune checkpoint in cancer and its therapeutic targeting.

Nature medicine

Xu Y,Poggio M,Jin HY,Shi Z,Forester CM,Wang Y,Stumpf CR,Xue L,Devericks E,So L,Nguyen HG,Griselin A,Gordan JD,Umetsu SE,Reich SH,Worland ST,Asthana S,Barna M,Webster KR,Cunningham JT,Ruggero D

Fri Feb 01 00:00:00 EST 2019

Translation control of the immune checkpoint in cancer and its therapeutic targeting.

Nature medicine

Xu Y,Poggio M,Jin HY,Shi Z,Forester CM,Wang Y,Stumpf CR,Xue L,Devericks E,So L,Nguyen HG,Griselin A,Gordan JD,Umetsu SE,Reich SH,Worland ST,Asthana S,Barna M,Webster KR,Cunningham JT,Ruggero D

Fri Feb 01 00:00:00 EST 2019

PHF7 is a novel histone H2A E3 ligase prior to histone-to-protamine exchange during spermiogenesis.

Development (Cambridge, England)

Wang X,Kang JY,Wei L,Yang X,Sun H,Yang S,Lu L,Yan M,Bai M,Chen Y,Long J,Li N,Li D,Huang J,Lei M,Shao Z,Yuan W,Zuo E,Lu K,Liu MF,Li J

Wed Jul 10 00:00:00 EDT 2019

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Biomaterials

Song H,Guo T,Zhao Z,Wei Y,Luo H,Weng W,Zhang R,Zhong M,Chen C,Su J,Shen W

Sat Sep 01 00:00:00 EDT 2018

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Biomaterials

Song H,Guo T,Zhao Z,Wei Y,Luo H,Weng W,Zhang R,Zhong M,Chen C,Su J,Shen W

Sat Sep 01 00:00:00 EDT 2018

Impact of iASPP on chemoresistance through PLK1 and autophagy in ovarian clear cell carcinoma.

International journal of cancer

Chan KK,Wong OG,Wong ES,Chan KK,Ip PP,Tse KY,Cheung AN

Sat Sep 15 00:00:00 EDT 2018

Impact of iASPP on chemoresistance through PLK1 and autophagy in ovarian clear cell carcinoma.

International journal of cancer

Chan KK,Wong OG,Wong ES,Chan KK,Ip PP,Tse KY,Cheung AN

Sat Sep 15 00:00:00 EDT 2018

pH-responsive hybrid nanoparticle with enhanced dissociation characteristic for siRNA delivery.

International journal of nanomedicine

Shi M,Zhao X,Zhang J,Pan S,Yang C,Wei Y,Hu H,Qiao M,Chen D,Zhao X

Fri Jan 04 00:00:00 EST 2019

pH-responsive hybrid nanoparticle with enhanced dissociation characteristic for siRNA delivery.

International journal of nanomedicine

Shi M,Zhao X,Zhang J,Pan S,Yang C,Wei Y,Hu H,Qiao M,Chen D,Zhao X

Fri Jan 04 00:00:00 EST 2019

pH-responsive hybrid nanoparticle with enhanced dissociation characteristic for siRNA delivery.

International journal of nanomedicine

Shi M,Zhao X,Zhang J,Pan S,Yang C,Wei Y,Hu H,Qiao M,Chen D,Zhao X

Fri Jan 04 00:00:00 EST 2019

pH-responsive hybrid nanoparticle with enhanced dissociation characteristic for siRNA delivery.

International journal of nanomedicine

Shi M,Zhao X,Zhang J,Pan S,Yang C,Wei Y,Hu H,Qiao M,Chen D,Zhao X

Fri Jan 04 00:00:00 EST 2019

Effects of PLK1 on proliferation, invasion and metastasis of gastric cancer cells through epithelial-mesenchymal transition.

Oncology letters

Song R,Hou G,Yang J,Yuan J,Wang C,Chai T,Liu Z

Thu Nov 01 00:00:00 EDT 2018

53BP1 and BRCA1 control pathway choice for stalled replication restart.

eLife

Xu Y,Ning S,Wei Z,Xu R,Xu X,Xing M,Guo R,Xu D

Mon Nov 06 00:00:00 EST 2017

Bioinformatics

Protein Aliases: cell cycle regulated protein kinase; PLK 1; PLK-1; polo (Drosophia)-like kinase; polo like kinase; polo-like kinase; Polo-like kinase 1; polo-like kinase homolog; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14

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Gene Aliases: PLK; PLK1; STPK13

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UniProt ID: (Human) P53350, (Mouse) Q07832, (Rat) Q62673

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Entrez Gene ID: (Human) 5347, (Mouse) 18817, (Rat) 25515

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Function(s)

protein kinase activity protein serine/threonine kinase activity protein binding ATP binding microtubule binding anaphase-promoting complex binding kinase activity protein kinase binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase protein modifying enzyme

Process(es)

mitotic sister chromatid segregation G2/M transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter mitotic cytokinesis cytokinesis microtubule bundle formation protein phosphorylation sister chromatid cohesion mitotic nuclear division mitotic nuclear envelope disassembly mitotic spindle assembly checkpoint regulation of mitotic cell cycle cell proliferation positive regulation of peptidyl-threonine phosphorylation female meiosis chromosome segregation protein ubiquitination peptidyl-serine phosphorylation regulation of mitotic metaphase/anaphase transition anaphase-promoting complex-dependent catabolic process G2 DNA damage checkpoint protein destabilization positive regulation of proteasomal ubiquitin-dependent protein catabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic process regulation of protein binding homologous chromosome segregation establishment of protein localization negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of proteolysis centrosome organization positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein transferase activity regulation of cell cycle synaptonemal complex disassembly protein localization to chromatin positive regulation of protein localization to nucleus regulation of mitotic spindle assembly regulation of cell cycle G2/M phase transition positive regulation of ubiquitin protein ligase activity cell cycle phosphorylation polar body extrusion after meiotic divisions cell division response to antibiotic

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PLK1 Polyclonal Antibody

PLK1 Antibody (10305-1-AP) in IHC (P)

Product Details

10305-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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