Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
PLK1 Antibody (PA5-28023) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of PLK1 was performed in HeLa cells fixed in 2% paraformadehyde at RT for 30 min. Green: PLK1 Polyclonal Antibody (Product # PA5-28023) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Product Details
PA5-28023
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 161 and 470 of Human PLK1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2545499
Product Specific Information
Recommended positive controls: A431, H1299, HepG2, Molt-4, Raji.
Predicted reactivity: Mouse (95%), Rat (95%), Chicken (80%), Bovine (95%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PLK1 is a serine/threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: cell cycle regulated protein kinase; PLK 1; PLK-1; polo (Drosophia)-like kinase; polo like kinase; polo-like kinase; Polo-like kinase 1; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14
Gene Aliases: PLK; PLK1; STPK13
UniProt ID: (Human) P53350, (Mouse) Q07832
Entrez Gene ID: (Human) 5347, (Mouse) 18817
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
microtubule binding
anaphase-promoting complex binding
kinase activity
protein kinase binding
nucleotide binding
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein modifying enzyme
mitotic sister chromatid segregation
G2/M transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
mitotic cytokinesis
cytokinesis
microtubule bundle formation
protein phosphorylation
sister chromatid cohesion
mitotic nuclear division
mitotic nuclear envelope disassembly
mitotic spindle assembly checkpoint
regulation of mitotic cell cycle
cell proliferation
positive regulation of peptidyl-threonine phosphorylation
female meiosis chromosome segregation
protein ubiquitination
peptidyl-serine phosphorylation
regulation of mitotic metaphase/anaphase transition
anaphase-promoting complex-dependent catabolic process
G2 DNA damage checkpoint
protein destabilization
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of apoptotic process
regulation of protein binding
homologous chromosome segregation
establishment of protein localization
negative regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of proteolysis
centrosome organization
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein transferase activity
regulation of cell cycle
synaptonemal complex disassembly
protein localization to chromatin
positive regulation of protein localization to nucleus
regulation of mitotic spindle assembly
regulation of cell cycle G2/M phase transition
positive regulation of ubiquitin protein ligase activity
cell cycle
phosphorylation
polar body extrusion after meiotic divisions
cell division
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support