Invitrogen

PLK1 Monoclonal Antibody (35-206)

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

11 Published Figures

23 References

View all (69) PLK1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

PLK1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 20

PLK1 Antibody (37-7000) in ICC/IF

Immunofluorescent staining of (top row) U2-OS cells, (bottom row) HeLa S3 cells using Ms anti PLK-1 (Product # 37-7000). Image courtesy of Dr. Erich Nigg, Max-Planck Institut fur Biochemie, Germany.

PLK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PLK1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

PLK1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with PLK1 siRNA and decrease in signal intensity was observed in western blot application (Fig a) using PLK1 Mouse Monoclonal Antibody (Product # 37-7000). Densitometric analysis of this western blot is shown in histogram (Fig b). {KD}

Product Details

37-7000

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

Assay-dependent

View 5 publications 5 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

35-206

Immunogen

Full-length human Plk1 (polo-like kinase 1)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533335

Target Information

PLK1 is a serine/threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Integrin-Mediated Adhesion Promotes Centrosome Separation in Early Mitosis.

Cells

Kamranvar SA,Gupta DK,Wasberg A,Liu L,Roig J,Johansson S

Published figure using PLK1 monoclonal antibody (Product # 37-7000) in Western Blot

Sat Apr 16 00:00:00 EDT 2022

A novel association of pyroptosis-related gene signature with the prognosis of hepatocellular carcinoma.

Frontiers in oncology

Li Y,Li Y,Zhang X,Duan X,Feng H,Yu Z,Gao Y

37-7000 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show that the three-PRG signature act as independent factor to predict the prognosis of HCC patients.

Sat Oct 22 00:00:00 EDT 2022

Protocol to identify centrosome-associated transcription factors during mitosis in mammalian cell lines.

STAR protocols

Xie G,Zhou Y,Du M,Wang Q,Yi J,Zhang XK

37-7000 was used in Immunocytochemistry to develop a new protocol demonstrating nuclear receptor RXRa centrosomal translocation which may be applied to other transcription factors.

Fri Sep 17 00:00:00 EDT 2021

Methylation of PLK1 by SET7/9 ensures accurate kinetochore-microtubule dynamics.

Journal of molecular cell biology

Yu R,Wu H,Ismail H,Du S,Cao J,Wang J,Ward T,Yang F,Gui P,Ali M,Chu L,Mo F,Wang Q,Chu Y,Zang J,Zhao Y,Ye M,Fang G,Chen PR,Dou Z,Gao X,Wang W,Liu X,Yao X

Published figure using PLK1 monoclonal antibody (Product # 37-7000) in Immunoprecipitation

Fri Jul 03 00:00:00 EDT 2020

Methylation of PLK1 by SET7/9 ensures accurate kinetochore-microtubule dynamics.

Journal of molecular cell biology

Yu R,Wu H,Ismail H,Du S,Cao J,Wang J,Ward T,Yang F,Gui P,Ali M,Chu L,Mo F,Wang Q,Chu Y,Zang J,Zhao Y,Ye M,Fang G,Chen PR,Dou Z,Gao X,Wang W,Liu X,Yao X

Published figure using PLK1 monoclonal antibody (Product # 37-7000) in Immunoprecipitation

Fri Jul 03 00:00:00 EDT 2020

Temporal and SUMO-specific SUMOylation contribute to the dynamics of Polo-like kinase 1 (PLK1) and spindle integrity during mouse oocyte meiosis.

Developmental biology

Feitosa WB,Hwang K,Morris PL

Published figure using PLK1 monoclonal antibody (Product # 37-7000) in Immunofluorescence

Thu Feb 15 00:00:00 EST 2018

YPC-21661 and YPC-22026, novel small molecules, inhibit ZNF143 activity in vitro and in vivo.

Cancer science

Haibara H,Yamazaki R,Nishiyama Y,Ono M,Kobayashi T,Hokkyo-Itagaki A,Nishisaka F,Nishiyama H,Kurita A,Matsuzaki T,Izumi H,Kohno K

37-7000 was used in Western Blotting to show that the inhibition of Zinc-finger protein 143 activity by small molecules induces tumour regression in vitro and in vivo.

Mon May 01 00:00:00 EDT 2017

A novel form of necrosis, TRIAD, occurs in human Huntington's disease.

Acta neuropathologica communications

Yamanishi E,Hasegawa K,Fujita K,Ichinose S,Yagishita S,Murata M,Tagawa K,Akashi T,Eishi Y,Okazawa H

Published figure using PLK1 monoclonal antibody (Product # 37-7000) in Immunofluorescence

Wed Mar 08 00:00:00 EST 2017

A novel form of necrosis, TRIAD, occurs in human Huntington's disease.

Acta neuropathologica communications

Yamanishi E,Hasegawa K,Fujita K,Ichinose S,Yagishita S,Murata M,Tagawa K,Akashi T,Eishi Y,Okazawa H

Published figure using PLK1 monoclonal antibody (Product # 37-7000) in Immunofluorescence

Wed Mar 08 00:00:00 EST 2017

Hypoxia-induced alterations of G2 checkpoint regulators.

Molecular oncology

Hasvold G,Lund-Andersen C,Lando M,Patzke S,Hauge S,Suo Z,Lyng H,Syljuåsen RG

37-7000 was used in flow cytometry to analyze G2 checkpoint regulators for hypoxia-induced alterations

Sun May 01 00:00:00 EDT 2016

Monitoring of changes in lipid profiles during PLK1 knockdown in cancer cells using DESI MS.

Analytical and bioanalytical chemistry

Jayashree B,Srimany A,Jayaraman S,Bhutra A,Janakiraman N,Chitipothu S,Krishnakumar S,Baddireddi LS,Elchuri S,Pradeep T

37-7000 was used in western blot to utilize DESI MS for PLK1 knockdown in cancer cells to monitor changes in lipid profiles

Mon Aug 01 00:00:00 EDT 2016

The hnRNP-Htt axis regulates necrotic cell death induced by transcriptional repression through impaired RNA splicing.

Cell death & disease

Mao Y,Tamura T,Yuki Y,Abe D,Tamada Y,Imoto S,Tanaka H,Homma H,Tagawa K,Miyano S,Okazawa H

37-7000 was used in western blot to study regulation of necrotic cell death induced by transcriptional repression through impaired RNA splicing by the hnRNP-Htt axis

Thu Apr 28 00:00:00 EDT 2016

Aberrant expression of maternal Plk1 and Dctn3 results in the developmental failure of human in-vivo- and in-vitro-matured oocytes.

Scientific reports

Fan Y,Zhao HC,Liu J,Tan T,Ding T,Li R,Zhao Y,Yan J,Sun X,Yu Y,Qiao J

37-7000 was used in western blot to compare gene expression profiles of oocytes and zygotes and focus on cytokinesis-related genes

Tue Feb 03 00:00:00 EST 2015

Aberrant expression of maternal Plk1 and Dctn3 results in the developmental failure of human in-vivo- and in-vitro-matured oocytes.

Scientific reports

Fan Y,Zhao HC,Liu J,Tan T,Ding T,Li R,Zhao Y,Yan J,Sun X,Yu Y,Qiao J

37-7000 was used in western blot to compare gene expression profiles of oocytes and zygotes and focus on cytokinesis-related genes

Tue Feb 03 00:00:00 EST 2015

Wee1 is required to sustain ATR/Chk1 signaling upon replicative stress.

Oncotarget

Saini P,Li Y,Dobbelstein M

37-7000 was used in western blot to compare the chemosensitizing effect of nucleoside analogues in cells derived from pancreatic cancer and in osteosarcoma-derived cells

Sat May 30 00:00:00 EDT 2015

SAPK pathways and p53 cooperatively regulate PLK4 activity and centrosome integrity under stress.

Nature communications

Nakamura T,Saito H,Takekawa M

37-7000 was used in western blot to study the regulation of polo-like kinase 4 activity and centrosome numeral integrity by SAPK pathways and p53.

Tue Nov 26 00:00:00 EST 2013

Proteomic profiling revealed the functional networks associated with mitotic catastrophe of HepG2 hepatoma cells induced by 6-bromine-5-hydroxy-4-methoxybenzaldehyde.

Toxicology and applied pharmacology

Zhang B,Huang B,Guan H,Zhang SM,Xu QZ,He XP,Liu XD,Wang Y,Shang ZF,Zhou PK

Sun May 01 00:00:00 EDT 2011

Proteomic profiling revealed the functional networks associated with mitotic catastrophe of HepG2 hepatoma cells induced by 6-bromine-5-hydroxy-4-methoxybenzaldehyde.

Toxicology and applied pharmacology

Zhang B,Huang B,Guan H,Zhang SM,Xu QZ,He XP,Liu XD,Wang Y,Shang ZF,Zhou PK

Sun May 01 00:00:00 EDT 2011

Three independent mechanisms for arrest in G2 after ionizing radiation.

Cell cycle (Georgetown, Tex.)

Landsverk KS,Patzke S,Rein ID,Stokke C,Lyng H,De Angelis PM,Stokke T

37-7000 was used in western blot to identify and characterize radiation-induced G2-arrest in human B-lymphocyte cancer cell lines.

Tue Mar 01 00:00:00 EST 2011

Forced Expression of ZNF143 Restrains Cancer Cell Growth.

Cancers

Izumi H,Yasuniwa Y,Akiyama M,Yamaguchi T,Kuma A,Kitamura N,Kohno K

37-7000 was used in Western Blotting to assess the function of ZNF143 expression in the cell cycle and cell division.

Wed Oct 19 00:00:00 EDT 2011

Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis.

Science signaling

Wang Z,Udeshi ND,Slawson C,Compton PD,Sakabe K,Cheung WD,Shabanowitz J,Hunt DF,Hart GW

Tue Jan 12 00:00:00 EST 2010

Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis.

Science signaling

Wang Z,Udeshi ND,Slawson C,Compton PD,Sakabe K,Cheung WD,Shabanowitz J,Hunt DF,Hart GW

Tue Jan 12 00:00:00 EST 2010

Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis.

Science signaling

Wang Z,Udeshi ND,Slawson C,Compton PD,Sakabe K,Cheung WD,Shabanowitz J,Hunt DF,Hart GW

Tue Jan 12 00:00:00 EST 2010

Cdk1/Erk2- and Plk1-dependent phosphorylation of a centrosome protein, Cep55, is required for its recruitment to midbody and cytokinesis.

Developmental cell

Fabbro M,Zhou BB,Takahashi M,Sarcevic B,Lal P,Graham ME,Gabrielli BG,Robinson PJ,Nigg EA,Ono Y,Khanna KK

37-7000 was used in immunocytochemistry, immunoprecipitation, and western blot to elucidate how Cep55 regulates cytokinesis

Sat Oct 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: cell cycle regulated protein kinase; PLK 1; PLK-1; polo (Drosophia)-like kinase; polo like kinase; polo-like kinase; Polo-like kinase 1; polo-like kinase homolog; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14

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Gene Aliases: PLK; PLK1; STPK13

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UniProt ID: (Human) P53350, (Mouse) Q07832, (Rat) Q62673

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Entrez Gene ID: (Human) 5347, (Mouse) 18817, (Rat) 25515

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Function(s)

protein kinase activity protein serine/threonine kinase activity protein binding ATP binding microtubule binding anaphase-promoting complex binding kinase activity protein kinase binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase protein modifying enzyme

Process(es)

mitotic sister chromatid segregation G2/M transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter mitotic cytokinesis cytokinesis microtubule bundle formation protein phosphorylation sister chromatid cohesion mitotic nuclear division mitotic nuclear envelope disassembly mitotic spindle assembly checkpoint regulation of mitotic cell cycle cell proliferation positive regulation of peptidyl-threonine phosphorylation female meiosis chromosome segregation protein ubiquitination peptidyl-serine phosphorylation regulation of mitotic metaphase/anaphase transition anaphase-promoting complex-dependent catabolic process G2 DNA damage checkpoint protein destabilization positive regulation of proteasomal ubiquitin-dependent protein catabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic process regulation of protein binding homologous chromosome segregation establishment of protein localization negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of proteolysis centrosome organization positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein transferase activity regulation of cell cycle synaptonemal complex disassembly protein localization to chromatin positive regulation of protein localization to nucleus regulation of mitotic spindle assembly regulation of cell cycle G2/M phase transition positive regulation of ubiquitin protein ligase activity cell cycle phosphorylation polar body extrusion after meiotic divisions cell division response to antibiotic

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PLK1 Monoclonal Antibody (35-206)

PLK1 Antibody (37-7000) in ICC/IF

Product Details

37-7000

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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