Invitrogen

PLK1 Monoclonal Antibody (36-298)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

9 Published Figures

18 References

View all (69) PLK1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

PLK1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

PLK1 Antibody (37-7100) in ICC/IF

Immunofluorescent staining of (top row) HeLa S3 and (bottom row) NIH 3T3 cells using Ms anti-PLK-1 (Product # 37-7100). Image courtesy of Dr. Erich Nigg, Max-Planck Institut fur Biochemie, Germany.

PLK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PLK1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

PLK1 Antibody — Downregulation of PLK1 reverses EMT and inhibits the motility of metastatic PCa cells. (A) Two metastatic PCa cell lines (DU145 and C4-2B) were infected with lentiviral shRNA constructs that target either the 3'-UTR of endogenous PLK1 (shPLK1#1) or serve as a control (shCTL). Wild-type (WT) or kinase-defective (KM) PLK1 were then re-expressed in PLK1 knockdown cells. The expression of PLK1 protein and EMT markers was determined by immunoblotting. In the PLK1 immunoblot, the lower bands show endogenous PLK1 expression and the upper bands show exogenous PLK1 expression. (B) Representative phase-contrast images of control, PLK1 knockdown cells, and PLK1 knockdown cells with re-expression of WT or KM PLK1. Scale bar = 50 µm. (C) The architecture of adherens junctions (E-cadherin) and tight junctions (JAM-A) in control cells and cells with PLK1 manipulation was evaluated by immunolabeling and confocal microscopy. Scale bar, 20 µm. (D, E) The effects of PLK1 knockdown on planar migration and invasion of PCa cells were measured by the wound healing assay (D) and Matrigel invasion assay (E), respectively. The data are presented as the mean s.e.m. *p<0.01, two-tailed Student's t-test. Scale bar, 500 µm (D), and 100 µm (E). {KD}

Product Details

37-7100

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Immunocytochemistry (ICC/IF)

Assay-dependent

View 6 publications 6 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 3 publications 3 publications

in situ PLA (PLA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

36-298

Immunogen

Full-length human Plk1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533336

Target Information

PLK1 is a serine/threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Polo-like kinase-1 mediates hepatitis C virus-induced cell migration, a drug target for liver cancer.

Life science alliance

El-Khobar KE,Tay E,Diefenbach E,Gloss BS,George J,Douglas MW

37-7100 was used in Immunocytochemistry-immunofluorescence to identify PLK1 as the mediating kinase.

Wed Nov 01 00:00:00 EDT 2023

Feedback control of PLK1 by Apolo1 ensures accurate chromosome segregation.

Cell reports

Xu L,Ali M,Duan W,Yuan X,Garba F,Mullen M,Sun B,Poser I,Duan H,Lu J,Tian R,Ge Y,Chu L,Pan W,Wang D,Hyman A,Green H,Li L,Dou Z,Liu D,Liu X,Yao X

37-7100 was used in Immunocytochemistry to identify Apolo1 as a tuner of PLK1 needed for correct kinetochore-microtubule attachments during cell division.

Tue Jul 13 00:00:00 EDT 2021

Feedback control of PLK1 by Apolo1 ensures accurate chromosome segregation.

Cell reports

Xu L,Ali M,Duan W,Yuan X,Garba F,Mullen M,Sun B,Poser I,Duan H,Lu J,Tian R,Ge Y,Chu L,Pan W,Wang D,Hyman A,Green H,Li L,Dou Z,Liu D,Liu X,Yao X

37-7100 was used in Immunocytochemistry to identify Apolo1 as a tuner of PLK1 needed for correct kinetochore-microtubule attachments during cell division.

Tue Jul 13 00:00:00 EDT 2021

Loss of aryl hydrocarbon receptor potentiates FoxM1 signaling to enhance self-renewal of colonic stem and progenitor cells.

The EMBO journal

Han H,Davidson LA,Fan YY,Goldsby JS,Yoon G,Jin UH,Wright GA,Landrock KK,Weeks BR,Wright RC,Allred CD,Jayaraman A,Ivanov I,Roper J,Safe SH,Chapkin RS

37-7100 was used in Western Blot to indicate that the AhR-FoxM1 axis, at least in part, mediates colonic stem/progenitor cell behavior.

Thu Oct 01 00:00:00 EDT 2020

Loss of aryl hydrocarbon receptor potentiates FoxM1 signaling to enhance self-renewal of colonic stem and progenitor cells.

The EMBO journal

Han H,Davidson LA,Fan YY,Goldsby JS,Yoon G,Jin UH,Wright GA,Landrock KK,Weeks BR,Wright RC,Allred CD,Jayaraman A,Ivanov I,Roper J,Safe SH,Chapkin RS

37-7100 was used in Western Blot to indicate that the AhR-FoxM1 axis, at least in part, mediates colonic stem/progenitor cell behavior.

Thu Oct 01 00:00:00 EDT 2020

O-GlcNAcylation of myosin phosphatase targeting subunit 1 (MYPT1) dictates timely disjunction of centrosomes.

The Journal of biological chemistry

Liu C,Shi Y,Li J,Liu X,Xiahou Z,Tan Z,Chen X,Li J

37-7100 was used in Immunoprecipitation to conclude that under high O-GlcNAc levels, PLK1 is untimely activated, conducive to inopportune centrosome separation and disruption of the cell cycle. We propose that too much O-GlcNAc is equally deleterious as too little O-GlcNAc, and a fine balance between the OGT/OGA duo is indispensable for successful mitotic divisions.

Fri May 22 00:00:00 EDT 2020

A PIM-CHK1 signaling pathway regulates PLK1 phosphorylation and function during mitosis.

Journal of cell science

Adam K,Cartel M,Lambert M,David L,Yuan L,Besson A,Mayeux P,Manenti S,Didier C

37-7100 was used in Proximity Ligation Assay to study the DNA damage response-independent roles of CHK1.

Fri Aug 10 00:00:00 EDT 2018

Albatross/FBF1 contributes to both centriole duplication and centrosome separation.

Genes to cells : devoted to molecular & cellular mechanisms

Inoko A,Yano T,Miyamoto T,Matsuura S,Kiyono T,Goshima N,Inagaki M,Hayashi Y

Published figure using PLK1 monoclonal antibody (Product # 37-7100) in Immunofluorescence

Sat Dec 01 00:00:00 EST 2018

Separase Inhibitor Sepin-1 Inhibits Foxm1 Expression and Breast Cancer Cell Growth.

Journal of cancer science & therapy

Zhang N,Pati D

37-7100 was used in Western Blotting to report that Sepin-1 hinders growth of breast cancer cells, cell migration, and wound healing.

Wed Sep 30 00:00:00 EDT 2020

Insulin Signaling Regulates the FoxM1/PLK1/CENP-A Pathway to Promote Adaptive Pancreatic β Cell Proliferation.

Cell metabolism

Shirakawa J,Fernandez M,Takatani T,El Ouaamari A,Jungtrakoon P,Okawa ER,Zhang W,Yi P,Doria A,Kulkarni RN

37-7100 was used in Immunohistochemistry to provide evidence for the involvement of the insulin-FoxM1/PLK1/CENP-A pathway in regulating pancreatic Β cell cell-cycle progression.

Tue Apr 04 00:00:00 EDT 2017

Phosphorylation of PP1 Regulator Sds22 by PLK1 Ensures Accurate Chromosome Segregation.

The Journal of biological chemistry

Duan H,Wang C,Wang M,Gao X,Yan M,Akram S,Peng W,Zou H,Wang D,Zhou J,Chu Y,Dou Z,Barrett G,Green HN,Wang F,Tian R,He P,Wang W,Liu X,Yao X

Published figure using PLK1 monoclonal antibody (Product # 37-7100) in Immunoprecipitation

Fri Sep 30 00:00:00 EDT 2016

Phosphorylation of PP1 Regulator Sds22 by PLK1 Ensures Accurate Chromosome Segregation.

The Journal of biological chemistry

Duan H,Wang C,Wang M,Gao X,Yan M,Akram S,Peng W,Zou H,Wang D,Zhou J,Chu Y,Dou Z,Barrett G,Green HN,Wang F,Tian R,He P,Wang W,Liu X,Yao X

Published figure using PLK1 monoclonal antibody (Product # 37-7100) in Immunoprecipitation

Fri Sep 30 00:00:00 EDT 2016

Polo-like kinase 1 induces epithelial-to-mesenchymal transition and promotes epithelial cell motility by activating CRAF/ERK signaling.

eLife

Wu J,Ivanov AI,Fisher PB,Fu Z

37-7100 was used in western blot to research induction of epithelial-to-mesenchymal transition by Polo-like kinase 1 and promotion of epithelial cell motility by activating CRAF/ERK signaling

Tue Mar 22 00:00:00 EDT 2016

FOXM1 and polo-like kinase 1 are co-ordinately overexpressed in patients with gastric adenocarcinomas.

BMC research notes

Dibb M,Han N,Choudhury J,Hayes S,Valentine H,West C,Sharrocks AD,Ang YS

37-7100 was used in immunohistochemistry to study co-expression of of FOXM1 and polo-like kinase 1 in gastric adenocarcinoma patients

Sat Nov 14 00:00:00 EST 2015

Calmodulin activation of polo-like kinase 1 is required during mitotic entry.

Biochemistry and cell biology = Biochimie et biologie cellulaire

Dai G,Qian Y,Chen J,Meng FL,Pan FY,Shen WG,Zhang SZ,Xue B,Li CJ

Tue Oct 01 00:00:00 EDT 2013

Calmodulin activation of polo-like kinase 1 is required during mitotic entry.

Biochemistry and cell biology = Biochimie et biologie cellulaire

Dai G,Qian Y,Chen J,Meng FL,Pan FY,Shen WG,Zhang SZ,Xue B,Li CJ

Tue Oct 01 00:00:00 EDT 2013

Polo-like kinase 1 regulates cell proliferation and is targeted by miR-593* in esophageal cancer.

International journal of cancer

Ito T,Sato F,Kan T,Cheng Y,David S,Agarwal R,Paun BC,Jin Z,Olaru AV,Hamilton JP,Selaru FM,Yang J,Matsumura N,Shimizu K,Abraham JM,Shimada Y,Mori Y,Meltzer SJ

37-7100 was used in western blot to determine the function and regulation of polo-like kinase 1 in esophageal cancer.

Tue Nov 01 00:00:00 EDT 2011

Polo-like kinase 1 regulates cell proliferation and is targeted by miR-593* in esophageal cancer.

International journal of cancer

Ito T,Sato F,Kan T,Cheng Y,David S,Agarwal R,Paun BC,Jin Z,Olaru AV,Hamilton JP,Selaru FM,Yang J,Matsumura N,Shimizu K,Abraham JM,Shimada Y,Mori Y,Meltzer SJ

37-7100 was used in western blot to determine the function and regulation of polo-like kinase 1 in esophageal cancer.

Tue Nov 01 00:00:00 EDT 2011

Disruptions of occludin and claudin-5 in brain endothelial cells in vitro and in brains of mice with acute liver failure.

Hepatology (Baltimore, Md.)

Chen F,Ohashi N,Li W,Eckman C,Nguyen JH

Published figure using PLK1 monoclonal antibody (Product # 37-7100) in Immunocytochemistry

Tue Dec 01 00:00:00 EST 2009

Bioinformatics

Protein Aliases: cell cycle regulated protein kinase; PLK 1; PLK-1; polo (Drosophia)-like kinase; polo like kinase; polo-like kinase; Polo-like kinase 1; polo-like kinase homolog; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14

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Gene Aliases: PLK; PLK1; STPK13

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UniProt ID: (Human) P53350, (Mouse) Q07832, (Rat) Q62673

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Entrez Gene ID: (Human) 5347, (Mouse) 18817, (Rat) 25515

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Function(s)

protein kinase activity protein serine/threonine kinase activity protein binding ATP binding microtubule binding anaphase-promoting complex binding kinase activity protein kinase binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase protein modifying enzyme

Process(es)

mitotic sister chromatid segregation G2/M transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter mitotic cytokinesis cytokinesis microtubule bundle formation protein phosphorylation sister chromatid cohesion mitotic nuclear division mitotic nuclear envelope disassembly mitotic spindle assembly checkpoint regulation of mitotic cell cycle cell proliferation positive regulation of peptidyl-threonine phosphorylation female meiosis chromosome segregation protein ubiquitination peptidyl-serine phosphorylation regulation of mitotic metaphase/anaphase transition anaphase-promoting complex-dependent catabolic process G2 DNA damage checkpoint protein destabilization positive regulation of proteasomal ubiquitin-dependent protein catabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic process regulation of protein binding homologous chromosome segregation establishment of protein localization negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of proteolysis centrosome organization positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein transferase activity regulation of cell cycle synaptonemal complex disassembly protein localization to chromatin positive regulation of protein localization to nucleus regulation of mitotic spindle assembly regulation of cell cycle G2/M phase transition positive regulation of ubiquitin protein ligase activity cell cycle phosphorylation polar body extrusion after meiotic divisions cell division response to antibiotic

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PLK1 Monoclonal Antibody (36-298)

PLK1 Antibody (37-7100) in ICC/IF

Product Details

37-7100

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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