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Invitrogen
PLK1 Monoclonal Antibody (3C11)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
Product Details
MA5-17152
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3C11
Immunogen
Purified recombinant fragment of human PLK1 (amino acids: 331-508) expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2538623
Product Specific Information
MA5-17152 targets PLK1 in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human and Mouse samples.
The MA5-17152 immunogen is purified recombinant fragment of human PLK1 (amino acids: 331-508) expressed in E. Coli.
MA5-17152 detects PLK1 which has a predicted molecular weight of approximately 68kDa.
Target Information
PLK1 is a serine/threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cell cycle regulated protein kinase; PLK 1; PLK-1; polo (Drosophia)-like kinase; polo like kinase; polo-like kinase; Polo-like kinase 1; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14
Gene Aliases: PLK; PLK1; STPK13
UniProt ID: (Human) P53350, (Mouse) Q07832
Entrez Gene ID: (Human) 5347, (Mouse) 18817
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
microtubule binding
anaphase-promoting complex binding
kinase activity
protein kinase binding
nucleotide binding
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein modifying enzyme
mitotic sister chromatid segregation
G2/M transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
mitotic cytokinesis
cytokinesis
microtubule bundle formation
protein phosphorylation
sister chromatid cohesion
mitotic nuclear division
mitotic nuclear envelope disassembly
mitotic spindle assembly checkpoint
regulation of mitotic cell cycle
cell proliferation
positive regulation of peptidyl-threonine phosphorylation
female meiosis chromosome segregation
protein ubiquitination
peptidyl-serine phosphorylation
regulation of mitotic metaphase/anaphase transition
anaphase-promoting complex-dependent catabolic process
G2 DNA damage checkpoint
protein destabilization
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of apoptotic process
regulation of protein binding
homologous chromosome segregation
establishment of protein localization
negative regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of proteolysis
centrosome organization
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein transferase activity
regulation of cell cycle
synaptonemal complex disassembly
protein localization to chromatin
positive regulation of protein localization to nucleus
regulation of mitotic spindle assembly
regulation of cell cycle G2/M phase transition
positive regulation of ubiquitin protein ligase activity
cell cycle
phosphorylation
polar body extrusion after meiotic divisions
cell division
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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