Product References
Linking post-translational modifications and protein turnover by site-resolved protein turnover profiling.
Nature communications
Zecha J,Gabriel W,Spallek R,Chang YC,Mergner J,Wilhelm M,Bassermann F,Kuster B
33-1700 was used in Western Blot to measure the turnover of >120,000 peptidoforms including >33,000 phosphorylated, acetylated, and ubiquitinated peptides for >9,000 native proteins.
Mon Jan 10 00:00:00 EST 2022
A kinase-independent function for AURORA-A in replisome assembly during DNA replication initiation.
Nucleic acids research
Guarino Almeida E,Renaudin X,Venkitaraman AR
33-1700 was used in Western Blotting to report a kinase-independent function for AURORA-A kinase(AURKA) in DNA replication initiation whose inhibition through a class of allosteric inhibitors opens avenues for cancer therapy.
Thu Aug 20 00:00:00 EDT 2020
Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival.
Nature communications
Dietachmayr M,Rathakrishnan A,Karpiuk O,von Zweydorf F,Engleitner T,Fernández-Sáiz V,Schenk P,Ueffing M,Rad R,Eilers M,Gloeckner CJ,Clemm von Hohenberg K,Bassermann F
33-1700 was used in Western Blot to find that CDC14B antagonizes CDK1-mediated activating mitotic phosphorylation of the deubiquitinase USP9X at serine residue 2563, which we show to be essential for USP9X to mediate mitotic survival.
Mon Mar 09 00:00:00 EDT 2020
Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival.
Nature communications
Dietachmayr M,Rathakrishnan A,Karpiuk O,von Zweydorf F,Engleitner T,Fernández-Sáiz V,Schenk P,Ueffing M,Rad R,Eilers M,Gloeckner CJ,Clemm von Hohenberg K,Bassermann F
33-1700 was used in Western Blot to find that CDC14B antagonizes CDK1-mediated activating mitotic phosphorylation of the deubiquitinase USP9X at serine residue 2563, which we show to be essential for USP9X to mediate mitotic survival.
Mon Mar 09 00:00:00 EDT 2020
Development of a Novel Cell-Permeable Protein-Protein Interaction Inhibitor for the Polo-box Domain of Polo-like Kinase 1.
ACS omega
Huggins DJ,Hardwick BS,Sharma P,Emery A,Laraia L,Zhang F,Narvaez AJ,Roberts-Thomson M,Crooks AT,Boyle RG,Boyce R,Walker DW,Mateu N,McKenzie GJ,Spring DR,Venkitaraman AR
33-1700 was used in Western Blotting to describe a novel compound series, which inhibits the protein-protein interactions of PLK1 via the polo-box domain.
Tue Jan 14 00:00:00 EST 2020
Preferential Killing of Tetraploid Colon Cancer Cells by Targeting the Mitotic Kinase PLK1.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
Jemaà M,Kifagi C,Serrano SS,Massoumi R
33-1700 was used in Western Blotting to report that PLK1 inhibition leads to the selective destruction of tetraploid cancer cells.
Wed Apr 08 00:00:00 EDT 2020
Clathrin heavy chain phosphorylated at T606 plays a role in proper cell division.
Cell cycle (Georgetown, Tex.)
Yabuno Y,Uchihashi T,Sasakura T,Shimizu H,Naito Y,Fukushima K,Ota K,Kogo M,Nojima H,Yabuta N
33-1700 was used in Immunocytochemistry-immunoflourescence to show cancer cell proliferation is supported by the GAK_CHC-pT606_PLK1_Kiz-pT379 axis.
Thu Aug 01 00:00:00 EDT 2019
A cryptic hydrophobic pocket in the polo-box domain of the polo-like kinase PLK1 regulates substrate recognition and mitotic chromosome segregation.
Scientific reports
Sharma P,Mahen R,Rossmann M,Stokes JE,Hardwick B,Huggins DJ,Emery A,Kunciw DL,Hyvönen M,Spring DR,McKenzie GJ,Venkitaraman AR
Published figure using PLK1 cocktail antibody (Product # 33-1700) in Western Blot
Mon Nov 04 00:00:00 EST 2019
HSP70 is required for the proper assembly of pericentriolar material and function of mitotic centrosomes.
Cell division
Fang CT,Kuo HH,Hsu SC,Yih LH
33-1700 was used in Immunocytochemistry-immunoflourescence to study the mechanisms that regulate centrosome expansion and maturation.
Fri Apr 08 00:00:00 EDT 2022
A PIM-CHK1 signaling pathway regulates PLK1 phosphorylation and function during mitosis.
Journal of cell science
Adam K,Cartel M,Lambert M,David L,Yuan L,Besson A,Mayeux P,Manenti S,Didier C
33-1700 was used in Western Blotting to study the DNA damage response-independent roles of CHK1.
Fri Aug 10 00:00:00 EDT 2018
CDC20B is required for deuterosome-mediated centriole production in multiciliated cells.
Nature communications
Revinski DR,Zaragosi LE,Boutin C,Ruiz-Garcia S,Deprez M,Thomé V,Rosnet O,Gay AS,Mercey O,Paquet A,Pons N,Ponzio G,Marcet B,Kodjabachian L,Barbry P
33-1700 was used in Immunohistochemistry-immunofluorescence to investigate the cell division cycle 20B gene in multiciliated cells.
Wed Nov 07 00:00:00 EST 2018
Plk1 overexpression induces chromosomal instability and suppresses tumor development.
Nature communications
de Cárcer G,Venkateswaran SV,Salgueiro L,El Bakkali A,Somogyi K,Rowald K,Montañés P,Sanclemente M,Escobar B,de Martino A,McGranahan N,Malumbres M,Sotillo R
33-1700 was used in Western Blotting to study the role of Plk1 in tumour development.
Wed Aug 01 00:00:00 EDT 2018
Biochemical analyses reveal amino acid residues critical for cell cycle-dependent phosphorylation of human Cdc14A phosphatase by cyclin-dependent kinase 1.
Scientific reports
Ovejero S,Ayala P,Malumbres M,Pimentel-Muiños FX,Bueno A,Sacristán MP
33-1700 was used in Western Blotting to examine the differences in Cyclin-Dependent Kinase1-mediated mechanisms of regulation between human and yeast Cdc14 orthologs.
Wed Aug 08 00:00:00 EDT 2018
A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability.
Cell
Tan SLW,Chadha S,Liu Y,Gabasova E,Perera D,Ahmed K,Constantinou S,Renaudin X,Lee M,Aebersold R,Venkitaraman AR
33-1700 was used in Western Blotting to describe a model wherein cacinogenesis in BRCA2 mutation carriers can be incited by compounds found in the environment.
Thu Jun 01 00:00:00 EDT 2017
A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability.
Cell
Tan SLW,Chadha S,Liu Y,Gabasova E,Perera D,Ahmed K,Constantinou S,Renaudin X,Lee M,Aebersold R,Venkitaraman AR
33-1700 was used in Western Blotting to describe a model wherein cacinogenesis in BRCA2 mutation carriers can be incited by compounds found in the environment.
Thu Jun 01 00:00:00 EDT 2017
Modulating Protein-Protein Interactions of the Mitotic Polo-like Kinases to Target Mutant KRAS.
Cell chemical biology
Narvaez AJ,Ber S,Crooks A,Emery A,Hardwick B,Guarino Almeida E,Huggins DJ,Perera D,Roberts-Thomson M,Azzarelli R,Hood FE,Prior IA,Walker DW,Boyce R,Boyle RG,Barker SP,Torrance CJ,McKenzie GJ,Venkitaraman AR
33-1700 was used in Western Blotting to show the utility of small molecules modulating the protein-protein interactions of polo-like kinases to therapeutically target mutant KRAS-expressing cancers.
Thu Aug 17 00:00:00 EDT 2017
USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma.
EMBO molecular medicine
Engel K,Rudelius M,Slawska J,Jacobs L,Ahangarian Abhari B,Altmann B,Kurutz J,Rathakrishnan A,Fernández-Sáiz V,Brunner A,Targosz BS,Loewecke F,Gloeckner CJ,Ueffing M,Fulda S,Pfreundschuh M,Trümper L,Klapper W,Keller U,Jost PJ,Rosenwald A,Peschel C,Bassermann F
33-1700 was used in western blot to investigate stabilization of XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma via USP9X
Mon Aug 01 00:00:00 EDT 2016
TOPBP1 regulates RAD51 phosphorylation and chromatin loading and determines PARP inhibitor sensitivity.
The Journal of cell biology
Moudry P,Watanabe K,Wolanin KM,Bartkova J,Wassing IE,Watanabe S,Strauss R,Troelsgaard Pedersen R,Oestergaard VH,Lisby M,Andújar-Sánchez M,Maya-Mendoza A,Esashi F,Lukas J,Bartek J
33-1700 was used in immunoprecipitation and western blot to investigate the role of topoisomerase IIbeta-binding protein 1 in DNA repair and its contribution to cancer
Mon Feb 01 00:00:00 EST 2016
TOPBP1 regulates RAD51 phosphorylation and chromatin loading and determines PARP inhibitor sensitivity.
The Journal of cell biology
Moudry P,Watanabe K,Wolanin KM,Bartkova J,Wassing IE,Watanabe S,Strauss R,Troelsgaard Pedersen R,Oestergaard VH,Lisby M,Andújar-Sánchez M,Maya-Mendoza A,Esashi F,Lukas J,Bartek J
33-1700 was used in immunoprecipitation and western blot to investigate the role of topoisomerase IIbeta-binding protein 1 in DNA repair and its contribution to cancer
Mon Feb 01 00:00:00 EST 2016
TOPBP1 regulates RAD51 phosphorylation and chromatin loading and determines PARP inhibitor sensitivity.
The Journal of cell biology
Moudry P,Watanabe K,Wolanin KM,Bartkova J,Wassing IE,Watanabe S,Strauss R,Troelsgaard Pedersen R,Oestergaard VH,Lisby M,Andújar-Sánchez M,Maya-Mendoza A,Esashi F,Lukas J,Bartek J
33-1700 was used in immunoprecipitation and western blot to investigate the role of topoisomerase IIbeta-binding protein 1 in DNA repair and its contribution to cancer
Mon Feb 01 00:00:00 EST 2016
Hypoxia-induced alterations of G2 checkpoint regulators.
Molecular oncology
Hasvold G,Lund-Andersen C,Lando M,Patzke S,Hauge S,Suo Z,Lyng H,Syljuåsen RG
33-1700 was used in western blot to analyze G2 checkpoint regulators for hypoxia-induced alterations
Sun May 01 00:00:00 EDT 2016
Genetic depletion of Polo-like kinase 1 leads to embryonic lethality due to mitotic aberrancies.
BioEssays : news and reviews in molecular, cellular and developmental biology
Wachowicz P,Fernández-Miranda G,Marugán C,Escobar B,de Cárcer G
Published figure using PLK1 cocktail antibody (Product # 33-1700) in Western Blot
Fri Jul 01 00:00:00 EDT 2016
High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells.
Molecular & cellular proteomics : MCP
Kanderova V,Kuzilkova D,Stuchly J,Vaskova M,Brdicka T,Fiser K,Hrusak O,Lund-Johansen F,Kalina T
331700 was used to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients
Fri Apr 01 00:00:00 EDT 2016
Pharmacological inhibition of Polo-like kinase 1 (PLK1) by BI-2536 decreases the viability and survival of hamartin and tuberin deficient cells via induction of apoptosis and attenuation of autophagy.
Cell cycle (Georgetown, Tex.)
Valianou M,Cox AM,Pichette B,Hartley S,Paladhi UR,Astrinidis A
33-1700 was used in western blot to elucidate how PLK1 regulates cell survival during mTORC1 hyperactivation.
Wed Dec 02 00:00:00 EST 2015
Pharmacological inhibition of Polo-like kinase 1 (PLK1) by BI-2536 decreases the viability and survival of hamartin and tuberin deficient cells via induction of apoptosis and attenuation of autophagy.
Cell cycle (Georgetown, Tex.)
Valianou M,Cox AM,Pichette B,Hartley S,Paladhi UR,Astrinidis A
33-1700 was used in western blot to elucidate how PLK1 regulates cell survival during mTORC1 hyperactivation.
Wed Dec 02 00:00:00 EST 2015
Pharmacological inhibition of Polo-like kinase 1 (PLK1) by BI-2536 decreases the viability and survival of hamartin and tuberin deficient cells via induction of apoptosis and attenuation of autophagy.
Cell cycle (Georgetown, Tex.)
Valianou M,Cox AM,Pichette B,Hartley S,Paladhi UR,Astrinidis A
33-1700 was used in western blot to elucidate how PLK1 regulates cell survival during mTORC1 hyperactivation.
Wed Dec 02 00:00:00 EST 2015
PLK1 regulation of PCNT cleavage ensures fidelity of centriole separation during mitotic exit.
Nature communications
Kim J,Lee K,Rhee K
33-1700 was used in western blot to assess the regulation of PLK1 and PCNT cleavage and mitotic exit via centriole separation
Wed Dec 09 00:00:00 EST 2015
SNW1 enables sister chromatid cohesion by mediating the splicing of sororin and APC2 pre-mRNAs.
The EMBO journal
van der Lelij P,Stocsits RR,Ladurner R,Petzold G,Kreidl E,Koch B,Schmitz J,Neumann B,Ellenberg J,Peters JM
33-1700 was used in western blot to demonstrate that SNW1 and PRPF8 are essential for sister chromatid cohesion in human cells.
Tue Nov 18 00:00:00 EST 2014
Human papillomavirus type 16 E7 oncoprotein inhibits the anaphase promoting complex/cyclosome activity by dysregulating EMI1 expression in mitosis.
Virology
Yu Y,Munger K
33-1700 was used in western blot to study the effect of human papillomavirus type 16 E7 oncoprotein on the anaphase promoting complex/cyclosome and its mechanism
Fri Nov 01 00:00:00 EDT 2013
NFBD1/MDC1 is phosphorylated by PLK1 and controls G2/M transition through the regulation of a TOPOIIα-mediated decatenation checkpoint.
PloS one
Ando K,Ozaki T,Hirota T,Nakagawara A
Published figure using PLK1 cocktail antibody (Product # 33-1700) in Western Blot
Thu Oct 09 00:00:00 EDT 2014
NFBD1/MDC1 is phosphorylated by PLK1 and controls G2/M transition through the regulation of a TOPOIIα-mediated decatenation checkpoint.
PloS one
Ando K,Ozaki T,Hirota T,Nakagawara A
Published figure using PLK1 cocktail antibody (Product # 33-1700) in Western Blot
Thu Oct 09 00:00:00 EDT 2014
NFBD1/MDC1 is phosphorylated by PLK1 and controls G2/M transition through the regulation of a TOPOIIα-mediated decatenation checkpoint.
PloS one
Ando K,Ozaki T,Hirota T,Nakagawara A
Published figure using PLK1 cocktail antibody (Product # 33-1700) in Western Blot
Thu Oct 09 00:00:00 EDT 2014
Human cells enter mitosis with damaged DNA after treatment with pharmacological concentrations of genotoxic agents.
The Biochemical journal
Kubara PM,Kernéis-Golsteyn S,Studény A,Lanser BB,Meijer L,Golsteyn RM
33-1700 was used in western blot to show that mitosis is a critical step in the cellular response to genotoxic agents in human cells
Sat Sep 15 00:00:00 EDT 2012
Human Cdc14A regulates Wee1 stability by counteracting CDK-mediated phosphorylation.
Molecular biology of the cell
Ovejero S,Ayala P,Bueno A,Sacristán MP
33-1700 was used in western blot to study the mechanism by which human Cdc4A regulates the stability of Wee.
Sat Dec 01 00:00:00 EST 2012
CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation.
The Journal of biological chemistry
Kashima L,Idogawa M,Mita H,Shitashige M,Yamada T,Ogi K,Suzuki H,Toyota M,Ariga H,Sasaki Y,Tokino T
Fri Apr 13 00:00:00 EDT 2012
CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation.
The Journal of biological chemistry
Kashima L,Idogawa M,Mita H,Shitashige M,Yamada T,Ogi K,Suzuki H,Toyota M,Ariga H,Sasaki Y,Tokino T
Fri Apr 13 00:00:00 EDT 2012
Phosphorylation of Ran-binding protein-1 by Polo-like kinase-1 is required for interaction with Ran and early mitotic progression.
The Journal of biological chemistry
Hwang HI,Ji JH,Jang YJ
33-1700 was used in immunocytochemistry and western blot to study phosphorylation of RanBP by Plk during mitosis.
Fri Sep 23 00:00:00 EDT 2011
Phosphorylation of Ran-binding protein-1 by Polo-like kinase-1 is required for interaction with Ran and early mitotic progression.
The Journal of biological chemistry
Hwang HI,Ji JH,Jang YJ
33-1700 was used in immunocytochemistry and western blot to study phosphorylation of RanBP by Plk during mitosis.
Fri Sep 23 00:00:00 EDT 2011
Phosphorylation of Ran-binding protein-1 by Polo-like kinase-1 is required for interaction with Ran and early mitotic progression.
The Journal of biological chemistry
Hwang HI,Ji JH,Jang YJ
33-1700 was used in immunocytochemistry and western blot to study phosphorylation of RanBP by Plk during mitosis.
Fri Sep 23 00:00:00 EDT 2011
Phosphorylation of Ran-binding protein-1 by Polo-like kinase-1 is required for interaction with Ran and early mitotic progression.
The Journal of biological chemistry
Hwang HI,Ji JH,Jang YJ
33-1700 was used in immunocytochemistry and western blot to study phosphorylation of RanBP by Plk during mitosis.
Fri Sep 23 00:00:00 EDT 2011
SP600125 suppresses Cdk1 and induces endoreplication directly from G2 phase, independent of JNK inhibition.
Oncogene
Kim JA,Lee J,Margolis RL,Fotedar R
Published figure using PLK1 cocktail antibody (Product # 33-1700) in Western Blot
Thu Mar 18 00:00:00 EDT 2010
Substrate degradation by the anaphase promoting complex occurs during mitotic slippage.
Cell cycle (Georgetown, Tex.)
Lee J,Kim JA,Margolis RL,Fotedar R
Sat May 01 00:00:00 EDT 2010
DNA damage triggers p21WAF1-dependent Emi1 down-regulation that maintains G2 arrest.
Molecular biology of the cell
Lee J,Kim JA,Barbier V,Fotedar A,Fotedar R
33-1700 was used in western blot to assess the role of p21(WAF1) in G2-M phase checkpoint control and in prevention of polyploidy after DNA damage
Wed Apr 01 00:00:00 EDT 2009
Plk1 phosphorylation of TRF1 is essential for its binding to telomeres.
The Journal of biological chemistry
Wu ZQ,Yang X,Weber G,Liu X
Published figure using PLK1 cocktail antibody (Product # 33-1700) in Immunoprecipitation
Fri Sep 12 00:00:00 EDT 2008
Plk1 phosphorylation of TRF1 is essential for its binding to telomeres.
The Journal of biological chemistry
Wu ZQ,Yang X,Weber G,Liu X
Published figure using PLK1 cocktail antibody (Product # 33-1700) in Immunoprecipitation
Fri Sep 12 00:00:00 EDT 2008
Plk1 phosphorylation of TRF1 is essential for its binding to telomeres.
The Journal of biological chemistry
Wu ZQ,Yang X,Weber G,Liu X
Published figure using PLK1 cocktail antibody (Product # 33-1700) in Immunoprecipitation
Fri Sep 12 00:00:00 EDT 2008
p73-mediated transcriptional activity is negatively regulated by polo-like kinase 1.
Cell cycle (Georgetown, Tex.)
Soond SM,Barry SP,Melino G,Knight RA,Latchman DS,Stephanou A
33-1700 was used in immunoprecipitation and western blot to report PLK1-mediated and phosphor-dependent transcriptional deactivation of TAp73
Thu May 01 00:00:00 EDT 2008
Destruction of Claspin by SCFbetaTrCP restrains Chk1 activation and facilitates recovery from genotoxic stress.
Molecular cell
Mailand N,Bekker-Jensen S,Bartek J,Lukas J
33-1700 was used in Western Blotting to suggest Claspin degradation facilitates timely reversal of the checkpoint response and delineates the period permissive for Chk1 activation during cell cycle progression.
Fri Aug 04 00:00:00 EDT 2006
CaMKII and polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit.
Proceedings of the National Academy of Sciences of the United States of America
Hansen DV,Tung JJ,Jackson PK
Tue Jan 17 00:00:00 EST 2006
Radiosensitization of colorectal carcinoma cell lines by histone deacetylase inhibition.
Radiation oncology (London, England)
Flatmark K,Nome RV,Folkvord S,Bratland A,Rasmussen H,Ellefsen MS,Fodstad Ø,Ree AH
33-1700 was used in western blot to assess if histone deacetylase inhibitors affect radiation-induced tumor cell death
Thu Aug 03 00:00:00 EDT 2006
Response of malignant B lymphocytes to ionizing radiation: gene expression and genotype.
International journal of cancer
Lyng H,Landsverk KS,Kristiansen E,DeAngelis PM,Ree AH,Myklebost O,Hovig E,Stokke T
33-1700 was used in western blot to determine the gene expression profiles of irradiated Reh and U698 cells
Wed Jul 20 00:00:00 EDT 2005
Plk1 regulates activation of the anaphase promoting complex by phosphorylating and triggering SCFbetaTrCP-dependent destruction of the APC Inhibitor Emi1.
Molecular biology of the cell
Hansen DV,Loktev AV,Ban KH,Jackson PK
33-1700 was used in immunocytochemistry and western blot to report that Plk1 is required for Emi1 destruction during mitosis
Wed Dec 01 00:00:00 EST 2004
Plk1 regulates activation of the anaphase promoting complex by phosphorylating and triggering SCFbetaTrCP-dependent destruction of the APC Inhibitor Emi1.
Molecular biology of the cell
Hansen DV,Loktev AV,Ban KH,Jackson PK
33-1700 was used in immunocytochemistry and western blot to report that Plk1 is required for Emi1 destruction during mitosis
Wed Dec 01 00:00:00 EST 2004
p53-regulated transcriptional program associated with genotoxic stress-induced apoptosis.
The Journal of biological chemistry
Kho PS,Wang Z,Zhuang L,Li Y,Chew JL,Ng HH,Liu ET,Yu Q
Fri May 14 00:00:00 EDT 2004
Mitotic regulation of the human anaphase-promoting complex by phosphorylation.
The EMBO journal
Kraft C,Herzog F,Gieffers C,Mechtler K,Hagting A,Pines J,Peters JM
Published figure using PLK1 cocktail antibody (Product # 33-1700) in Western Blot
Mon Dec 15 00:00:00 EST 2003
Mitotic regulation of the human anaphase-promoting complex by phosphorylation.
The EMBO journal
Kraft C,Herzog F,Gieffers C,Mechtler K,Hagting A,Pines J,Peters JM
Published figure using PLK1 cocktail antibody (Product # 33-1700) in Western Blot
Mon Dec 15 00:00:00 EST 2003
The G(2) DNA damage checkpoint delays expression of genes encoding mitotic regulators.
The Journal of biological chemistry
Crawford DF,Piwnica-Worms H
33-1700 was used in western blot to assess global gene expression using p53-deficient human cells to identify genes with G(2)/M-specific and DNA damage-responsive expression.
Fri Oct 05 00:00:00 EDT 2001
