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Product Details
ACP-002-200UL
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)KEIPDPSSINAKTLE, corresponding to amino acid residues 522-536 of rat PMCA2, 2ndintracellular loop
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/K+ pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATPase isoform 2 Na+K+ transporting beta polypeptide 2; ATPase isoform 2, Na+K+ transporting, beta polypeptide 2; deaf waddler; plasma membrane Ca++-ATPase; plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 2; plasma membrane calcium pump; Plasma membrane calcium pump isoform 2; Plasma membrane calcium-transporting ATPase 2; PMCA2; wriggle
Gene Aliases: Atp2b2; D6Abb2e; dfw; Gena300; jog; PMCA2; Tmy; wms; wri
UniProt ID: (Rat) P11506, (Mouse) Q9R0K7
Entrez Gene ID: (Rat) 24215, (Mouse) 11941
calcium-transporting ATPase activity
calcium ion binding
calmodulin binding
ATP binding
protein C-terminus binding
PDZ domain binding
calcium-dependent ATPase activity
acetylcholine receptor binding
glutamate receptor binding
metal ion binding
nucleotide binding
hydrolase activity
primary active transporter
cell morphogenesis
neural retina development
calcium ion transport
cellular calcium ion homeostasis
organelle organization
brain development
lactation
sensory perception of sound
locomotory behavior
metabolic process
regulation of cell size
cerebellum development
cerebellar Purkinje cell layer morphogenesis
cerebellar Purkinje cell differentiation
cerebellar granule cell differentiation
neuron differentiation
locomotion
serotonin metabolic process
inner ear morphogenesis
otolith mineralization
cGMP metabolic process
regulation of synaptic plasticity
inner ear development
synapse organization
neuromuscular process controlling balance
detection of mechanical stimulus involved in sensory perception of sound
regulation of cytosolic calcium ion concentration
positive regulation of calcium ion transport
auditory receptor cell stereocilium organization
inner ear receptor cell differentiation
calcium ion transmembrane transport
cochlea development
calcium ion export
transport
ion transport
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