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Invitrogen
PMEL Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
PMEL Antibody (PA5-32491) in WB
Western blot was performed using Anti-PMEL Polyclonal Antibody (Product # PA5-32491) and an 80 kDa band corresponding to PMEL was observed across in SK-MEL-5 in comparison to A-375 and SK-BR-3 which is supposed to be negative. A non-specific band around 58 kDa was also observed. Whole cell extracts (30 µg lysate) of SK-MEL-5 (Lane 1), A-375 (Lane 2) and SK-BR-3 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB230... View More
Product Details
PA5-32491
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encoding aa 1-150 human gp100.
Conjugate
Form
Liquid
Concentration
0.123 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.6, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2549958
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is melanoma.
Target Information
Melanoma is a malignant tumor of melanocytes which are found predominantly in skin but also in the bowel and the eye (see uveal melanoma). It is one of the rarer types of skin cancer but causes the majority of skin cancer related deaths.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 95kDa melanocyte-specific secreted glycoprotein; ME20-M; ME20M; Melanocyte lineage specific antigen GP100; melanocyte protein mel 17; Melanocyte protein PMEL; Melanocyte protein Pmel 17; Melanocytes lineage-specific antigen GP100; Melanoma-associated ME20 antigen; melanosomal matrix protein17; P1; P100; Pmel 17; Premelanosome protein; Silver locus protein homolog; silver, mouse, homolog of
Gene Aliases: D12S53E; gp100; ME20; ME20-M; ME20M; P1; P100; PMEL; PMEL17; SI; SIL; SILV
UniProt ID: (Human) P40967
Entrez Gene ID: (Human) 6490
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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