Invitrogen

PODXL Monoclonal Antibody (3D3)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

7 Published Figures

21 References

View all (46) Podocalyxin (PODXL) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

PODXL Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

PODXL Antibody (39-3800) in ICC/IF

Immunofluorescence analysis of PODXL was performed using 70% confluent log phase NCCIT and A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PODXL Monoclonal Antibody (3D3) (Product # 39-3800) at 2 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A3... View More

PODXL Antibody in Immunohistochemistry (IHC)

Product Image Gallery

PODXL Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-PODXL Monoclonal Antibody (3D3) (Product # 393800) shows increased expression of PODXL in NCCIT cell line when compared to A549. {RE}

Product Details

39-3800

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:2,000

View 7 publications 7 publications

Immunoprecipitation (IP)

Assay-dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Dog, Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3D3

Immunogen

Human podocalyxin fusion protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533411

Target Information

This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

GlomSpheres as a 3D co-culture spheroid model of the kidney glomerulus for rapid drug-screening.

Communications biology

Tuffin J,Chesor M,Kuzmuk V,Johnson T,Satchell SC,Welsh GI,Saleem MA

39-3800 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot to demonstrate a dramatic upregulation of key podocyte (podocin, nephrin and podocalyxin) and GEnC (pecam-1) markers. Electron microscopy revealed podocyte foot process interdigitation and endothelial vessel formation.

Thu Dec 02 00:00:00 EST 2021

3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening.

Nature communications

Hale LJ,Howden SE,Phipson B,Lonsdale A,Er PX,Ghobrial I,Hosawi S,Wilson S,Lawlor KT,Khan S,Oshlack A,Quinlan C,Lennon R,Little MH

39-3800 was used in Immunohistochemistry-immunofluorescence to compare 3D human glomeruli sieved from induced pluripotent stem cell-derived kidney organoids with conditionally immortalised human podocyte cell lines.

Tue Dec 04 00:00:00 EST 2018

3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening.

Nature communications

Hale LJ,Howden SE,Phipson B,Lonsdale A,Er PX,Ghobrial I,Hosawi S,Wilson S,Lawlor KT,Khan S,Oshlack A,Quinlan C,Lennon R,Little MH

Tue Dec 04 00:00:00 EST 2018

Therapy with atorvastatin versus rosuvastatin reduces urinary podocytes, podocyte-associated molecules, and proximal tubule dysfunction biomarkers in patients with type 2 diabetes mellitus: a pilot study.

Renal failure

Vlad A,Vlad M,Petrica L,Ursoniu S,Gadalean F,Popescu R,Vlad D,Dumitrascu V,Gluhovschi G,Gluhovschi C,Velciov S,Bob F,Matusz P,Secara A,Simulescu A,Jianu DC

39-3800 was used in Immunocytochemistry-immunoflourescence to determine if atorvastatin protects the podocytes and the proximal tubule in patients with Type 2 diabetes.

Wed Nov 01 00:00:00 EDT 2017

Urinary podocyte-associated mRNA levels correlate with proximal tubule dysfunction in early diabetic nephropathy of type 2 diabetes mellitus.

Diabetology & metabolic syndrome

Petrica L,Ursoniu S,Gadalean F,Vlad A,Gluhovschi G,Dumitrascu V,Vlad D,Gluhovschi C,Velciov S,Bob F,Matusz P,Milas O,Secara A,Simulescu A,Popescu R

39-3800 was used in Immunocytochemistry-immunoflourescence to assess mRNA expression of podocyte-associated molecules in urinary sediments of patients with type 2 diabetes mellitus in relation to urinary podocytes.

Fri Mar 18 00:00:00 EDT 2022

Zonula occludens-2 regulates Rho proteins activity and the development of epithelial cytoarchitecture and barrier function.

Biochimica et biophysica acta. Molecular cell research

Raya-Sandino A,Castillo-Kauil A,Domínguez-Calderón A,Alarcón L,Flores-Benitez D,Cuellar-Perez F,López-Bayghen B,Chávez-Munguía B,Vázquez-Prado J,González-Mariscal L

39-3800 was used in Immunocytochemistry to show that ZO-2 is a crucial regulator of Rho proteins activity and the development of epithelial cytoarchitecture and barrier function.

Sun Oct 01 00:00:00 EDT 2017

Urinary Podocyte Loss Is Increased in Patients with Fabry Disease and Correlates with Clinical Severity of Fabry Nephropathy.

PloS one

Fall B,Scott CR,Mauer M,Shankland S,Pippin J,Jefferson JA,Wallace E,Warnock D,Najafian B

393800 was used in immunocytochemistry to ask if podocyturia increases in Fabry patients and correlates with clinical severity of Fabry nephropathy

Wed Jul 05 00:00:00 EDT 2017

Urinary Podocyte Loss Is Increased in Patients with Fabry Disease and Correlates with Clinical Severity of Fabry Nephropathy.

PloS one

Fall B,Scott CR,Mauer M,Shankland S,Pippin J,Jefferson JA,Wallace E,Warnock D,Najafian B

393800 was used in immunocytochemistry to ask if podocyturia increases in Fabry patients and correlates with clinical severity of Fabry nephropathy

Wed Jul 05 00:00:00 EDT 2017

Podocalyxin promotes glioblastoma multiforme cell invasion and proliferation by inhibiting angiotensin-(1-7)/Mas signaling.

Oncology reports

Liu B,Liu Y,Jiang Y

39-3800 was used in western blot to investigate the effect of crosstalk between PODX and Ang-(1-7)/Mas signaling in glioblastoma multiforme cells

Fri May 01 00:00:00 EDT 2015

Podocalyxin promotes cisplatin chemoresistance in osteosarcoma cells through phosphatidylinositide 3-kinase signaling.

Molecular medicine reports

Huang Z,Huang Y,He H,Ni J

39-3800 was used in immunocytochemistry assess the role of podocalyxin in osteosarcoma

Tue Sep 01 00:00:00 EDT 2015

Bmi1 essentially mediates podocalyxin-enhanced Cisplatin chemoresistance in oral tongue squamous cell carcinoma.

PloS one

Zhou Y,Zhang L,Pan H,Wang B,Yan F,Fang X,Munnee K,Tang Z

39-3800 was used in western blot to study the molecular basis for cisplatin chemoresistance in oral tongue squamous cell carcinoma

Mon Apr 11 00:00:00 EDT 2016

Sperm‑associated antigen 9 promotes astrocytoma cell invasion through the upregulation of podocalyxin.

Molecular medicine reports

Jiang J,Liu Y,Fang W,Liu F

39-3800 was used in western blot to study the relationship between SPAG9 and PODXL in human astrocytoma invasion

Tue Jul 01 00:00:00 EDT 2014

Podocalyxin promotes glioblastoma multiforme cell invasion and proliferation via β-catenin signaling.

PloS one

Liu Y,Yang L,Liu B,Jiang YG

39-3800 was used in western blot to explored the impact of crosstalk between PODX and beta-cat signaling in glioblastoma multiform cells

Fri Jan 08 00:00:00 EST 2016

Stimulatory effects of cardiotrophin 1 on atherosclerosis.

Hypertension (Dallas, Tex. : 1979)

Konii H,Sato K,Kikuchi S,Okiyama H,Watanabe R,Hasegawa A,Yamamoto K,Itoh F,Hirano T,Watanabe T

Fri Nov 01 00:00:00 EDT 2013

Role of podocalyxin in astrocytoma: Clinicopathological and in vitro evidence.

Oncology letters

Huang T,Jin X,He L,Zhang M,Wu J,Wang Y,Fang J

Published figure using PODXL monoclonal antibody (Product # 39-3800) in Immunohistochemistry

Fri Nov 01 00:00:00 EDT 2013

Podocalyxin regulates astrocytoma cell invasion and survival against temozolomide.

Experimental and therapeutic medicine

Wu H,Yang L,Liao D,Chen Y,Wang W,Fang J

39-3800 was used in western blot to test the effect of PODXL on astrocytoma cell invasion and survival against a chemotherapy agent.

Mon Apr 01 00:00:00 EDT 2013

A dimeric PINK1-containing complex on depolarized mitochondria stimulates Parkin recruitment.

The Journal of biological chemistry

Okatsu K,Uno M,Koyano F,Go E,Kimura M,Oka T,Tanaka K,Matsuda N

39-3800 was used in immunohistochemistry and western blot to investigate the molecular mechanism that result in PINK1-mediated dissipation of the mitochondrial membrane potential.

Fri Dec 20 00:00:00 EST 2013

PINCH1 is transcriptional regulator in podocytes that interacts with WT1 and represses podocalyxin expression.

PloS one

Wang D,Li Y,Wu C,Liu Y

39-3800 was used in western blot to determine if PINCH1 translocates to the nucleus and regulates gene expression.

Thu Feb 24 00:00:00 EST 2011

Overexpression of the anti-adhesin podocalyxin is an independent predictor of breast cancer progression.

Cancer research

Somasiri A,Nielsen JS,Makretsov N,McCoy ML,Prentice L,Gilks CB,Chia SK,Gelmon KA,Kershaw DB,Huntsman DG,McNagny KM,Roskelley CD

Sun Aug 01 00:00:00 EDT 2004

Enhanced podocalyxin expression alters the structure of podocyte basal surface.

Journal of cell science

Economou CG,Kitsiou PV,Tzinia AK,Panagopoulou E,Marinos E,Kershaw DB,Kerjaschki D,Tsilibary EC

Thu Jul 01 00:00:00 EDT 2004

Human embryonal carcinoma tumor antigen, Gp200/GCTM-2, is podocalyxin.

Biochemical and biophysical research communications

Schopperle WM,Kershaw DB,DeWolf WC

Fri Jan 10 00:00:00 EST 2003

Human embryonal carcinoma tumor antigen, Gp200/GCTM-2, is podocalyxin.

Biochemical and biophysical research communications

Schopperle WM,Kershaw DB,DeWolf WC

Fri Jan 10 00:00:00 EST 2003

Bioinformatics

Protein Aliases: GCTM-2 antigen; Gp200; MGC138240; PC; PCLP antibody; PCLP1 antibody; PODLX2; Podocalyxin; podocalyxin-like; Podocalyxin-like protein 1; podolyxin

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Gene Aliases: Gp200; PC; PCLP; PCLP-1; PCLP1; PODXL

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UniProt ID: (Human) O00592

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Entrez Gene ID: (Human) 5420

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Function(s)

protein binding cell adhesion molecule

Process(es)

cell adhesion negative regulation of cell adhesion cell migration negative regulation of cell-cell adhesion positive regulation of cell migration regulation of microvillus assembly positive regulation of cell-cell adhesion mediated by integrin leukocyte migration glomerular visceral epithelial cell development epithelial tube formation

Performance Guarantee

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PODXL Monoclonal Antibody (3D3)

PODXL Antibody (39-3800) in ICC/IF

Product Details

39-3800

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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