Proteintech

POFUT1 Polyclonal Antibody

POFUT1 Antibody in Immunocytochemistry (ICC/IF)

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POFUT1 Antibody (14929-1-AP) in ICC/IF

Immunofluorescent analysis of A431 cells, using POFUT1 antibody 14929-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

POFUT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

POFUT1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14929-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 5 publications 5 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

POFUT1 Fusion Protein Ag6710 (1-182 aa encoded by BC000582)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MGAAAWARP LSVSFLLLLL PLPGMPAGSW DPAGYLLYCP CMGRFGNQAD HFLGSLAFAK LLNRTLAVPP WIEYQHHKPP FTNLHVSYQK YFKLEPLQAY HRVISLEDFM EKLAPTHWPP EKRVAYCFEV AAQRSPDKKT CPMKEGNPFG PFWDQFHVSF NKSELFTGIS FSASYREQWS QRR (1-182 aa encoded by BC000582)

Target Information

POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch; mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8⁺ T cell fate decisions.

Cell

Huang H,Zhou P,Wei J,Long L,Shi H,Dhungana Y,Chapman NM,Fu G,Saravia J,Raynor JL,Liu S,Palacios G,Wang YD,Qian C,Yu J,Chi H

Thu Mar 04 00:00:00 EST 2021

A spoonful of L-fucose-an efficient therapy for GFUS-CDG, a new glycosylation disorder.

EMBO molecular medicine

Feichtinger RG,Hüllen A,Koller A,Kotzot D,Grote V,Rapp E,Hofbauer P,Brugger K,Thiel C,Mayr JA,Wortmann SB

Tue Sep 07 00:00:00 EDT 2021

Epiregulin promotes trophoblast epithelial-mesenchymal transition through poFUT1 and O-fucosylation by poFUT1 on uPA.

Cell proliferation

Cui X,Wang H,Li Y,Chen T,Liu S,Yan Q

Sat Feb 01 00:00:00 EST 2020

poFUT1 promotes uterine angiogenesis and vascular remodeling via enhancing the O-fucosylation on uPA.

Cell death & disease

Zhang D,Yang Y,Liang C,Liu J,Wang H,Liu S,Yan Q

Thu Oct 10 00:00:00 EDT 2019

poFUT1 promotes uterine angiogenesis and vascular remodeling via enhancing the O-fucosylation on uPA.

Cell death & disease

Zhang D,Yang Y,Liang C,Liu J,Wang H,Liu S,Yan Q

Thu Oct 10 00:00:00 EDT 2019

PLAGL2 and POFUT1 are regulated by an evolutionarily conserved bidirectional promoter and are collaboratively involved in colorectal cancer by maintaining stemness.

EBioMedicine

Li D,Lin C,Li N,Du Y,Yang C,Bai Y,Feng Z,Su C,Wu R,Song S,Yan P,Chen M,Jain A,Huang L,Zhang Y,Li X

Mon Jul 01 00:00:00 EDT 2019

PLAGL2 and POFUT1 are regulated by an evolutionarily conserved bidirectional promoter and are collaboratively involved in colorectal cancer by maintaining stemness.

EBioMedicine

Li D,Lin C,Li N,Du Y,Yang C,Bai Y,Feng Z,Su C,Wu R,Song S,Yan P,Chen M,Jain A,Huang L,Zhang Y,Li X

Mon Jul 01 00:00:00 EDT 2019

PLAGL2 and POFUT1 are regulated by an evolutionarily conserved bidirectional promoter and are collaboratively involved in colorectal cancer by maintaining stemness.

EBioMedicine

Li D,Lin C,Li N,Du Y,Yang C,Bai Y,Feng Z,Su C,Wu R,Song S,Yan P,Chen M,Jain A,Huang L,Zhang Y,Li X

Mon Jul 01 00:00:00 EDT 2019

Progesterone promotes embryo adhesion by upregulating c-Fos/c-Jun transcription factor-mediated poFUT1 expression†.

Biology of reproduction

Cui X,Sun J,Liang C,Zheng Q,Yang X,Liu S,Yan Q

Fri Oct 25 00:00:00 EDT 2019

Progesterone promotes embryo adhesion by upregulating c-Fos/c-Jun transcription factor-mediated poFUT1 expression†.

Biology of reproduction

Cui X,Sun J,Liang C,Zheng Q,Yang X,Liu S,Yan Q

Fri Oct 25 00:00:00 EDT 2019

POFUT1 promotes colorectal cancer development through the activation of Notch1 signaling.

Cell death & disease