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POFUT1 Antibody (H00023509-D01P) in WB
Western Blot analysis of POFUT1 expression in transfected 293T cell line (H00023509-T02) by POFUT1 MaxPab polyclonal antibody.
Lane 1: POFUT1 transfected lysate(22.30 KDa).
Lane 2: Non-transfected lysate.
Lane 1: POFUT1 transfected lysate(22.30 KDa).
Lane 2: Non-transfected lysate.
Product Details
H00023509-D01P
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
POFUT1 (NP_758436.1, 1 a.a. approximately 194 a.a) full-length human protein.
Conjugate
Form
Liquid
Concentration
See Label
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Sequence of this protein is as follows: MGAAAWARPL SVSFLLLLLP LPGMPAGSWD PAGYLLYCPC MGRFGNQADH FLGSLAFAKL LNRTLAVPPW IEYQHHKPPF TNLHVSYQKY FKLEPLQAYH RVISLEDFME KLAPTHWPPE KRVAYCFEVA AQRSPDKKTC PMKEGNPFGP FWDQFHVSFN KSELFTGISF SASYREQWSQ RRENHSCVTL LFPR
Target Information
POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch; mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GDP-fucose protein O-fucosyltransferase 1; o-fucosyltransferase protein; O-FucT-1; Peptide-O-fucosyltransferase 1
Gene Aliases: DDD2; FUT12; KIAA0180; mKIAA0180; O-Fuc-T; O-FucT-1; O-FUT; OFUCT1; POFUT1
UniProt ID: (Human) Q9H488, (Mouse) Q91ZW2
Entrez Gene ID: (Human) 23509, (Mouse) 140484
angiogenesis
somitogenesis
fucose metabolic process
regulation of transcription, DNA-templated
protein O-linked glycosylation
Notch signaling pathway
nervous system development
heart development
embryo development
O-glycan processing
protein O-linked fucosylation
carbohydrate metabolic process
fucosylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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