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FIGURE: 1 / 7
POLB Antibody (MA5-47074) in ICC/IF
Immunofluorescent analysis of POLB in HeLa cells. Cells were fixed in ice-cold MeOH for 10 min. Green: DNA polymerase beta stained by DNA polymerase beta monoclonal antibody (Product # MA5-47074) diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1,000. Blue: Fluoroshield with DAPI Scale bar= 10 µm.
Product Details
MA5-47074
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
HL1512
Immunogen
Recombinant protein encompassing a sequence within the center region of human DNA polymerase beta. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2938146
Product Specific Information
Store as concentrated solution.
Centrifuge briefly prior to opening vial.
Target Information
DNA polymerase beta comprises an amino-terminal 8- kDa domain and a carboxy-terminal 31- kDa domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyltransferase activity. Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment of cells with the DNA-alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5'-deoxyribose-phosphate lyase; 5'-dRP lyase; AP lyase; DNA pol beta; DNA polymerase beta; DNA polymerase beta subunit; DNA-directed DNA polymerase beta; MGC125976; POL B; Pol beta; polymerase (DNA directed), beta; polymerase (DNA) beta; polymerase B; polymerase-beta
Gene Aliases: A430088C08Rik; POLB; wu:fa97a05; zgc:109924
UniProt ID: (Human) P06746, (Rat) P06766, (Mouse) Q8K409
Entrez Gene ID: (Human) 5423, (Rat) 29240, (Mouse) 18970, (Zebrafish) 554204
damaged DNA binding
DNA-directed DNA polymerase activity
DNA-(apurinic or apyrimidinic site) lyase activity
protein binding
microtubule binding
lyase activity
enzyme binding
metal ion binding
DNA binding
transferase activity
nucleotidyltransferase activity
DNA polymerase activity
DNA-directed DNA polymerase
DNA-dependent DNA replication
DNA repair
base-excision repair
base-excision repair, base-free sugar-phosphate removal
base-excision repair, gap-filling
base-excision repair, DNA ligation
pyrimidine dimer repair
nucleotide-excision repair, DNA gap filling
inflammatory response
cellular response to DNA damage stimulus
salivary gland morphogenesis
aging
intrinsic apoptotic signaling pathway in response to DNA damage
response to gamma radiation
somatic hypermutation of immunoglobulin genes
response to ethanol
lymph node development
spleen development
homeostasis of number of cells
neuron apoptotic process
response to hyperoxia
immunoglobulin heavy chain V-D-J recombination
DNA biosynthetic process
DNA replication
apoptotic process
somatic diversification of immunoglobulins
double-strand break repair via nonhomologous end joining
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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