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POLD1 Antibody (PA5-34678) in IHC (P)
POLD1 Polyclonal Antibody detects DNA polymerase delta protein at cytoplasm and nucleus on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. POLD1 Polyclonal Antibody (Product # PA5-34678) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-34678
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 685 and 1071 of DNA pol delta cat (Uniprot ID#P28340)
Conjugate
Form
Liquid
Concentration
0.56 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2552030
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, Molt-4, Rat2.
Predicted reactivity: Mouse (92%), Rat (91%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3'-5' exodeoxyribonuclease; CDC2 homolog; DNA polymerase delta catalytic subunit; DNA polymerase delta, catalytic subunit; DNA polymerase subunit delta p125; DNA-directed DNA polymerase delta 1; POL D; polymerase (DNA directed), delta 1, catalytic subunit 125kDa; polymerase (DNA) delta 1, catalytic subunit; Polymerase D
Gene Aliases: CDC2; CRCS10; MDPL; POLD; POLD1
UniProt ID: (Human) P28340, (Rat) O54747
Entrez Gene ID: (Human) 5424, (Rat) 59294
telomere maintenance via recombination
telomere maintenance
DNA synthesis involved in DNA repair
DNA replication
DNA strand elongation involved in DNA replication
DNA repair
transcription-coupled nucleotide-excision repair
base-excision repair, gap-filling
nucleotide-excision repair, DNA incision, 5'-to lesion
nucleotide-excision repair, DNA gap filling
mismatch repair
response to UV
translesion synthesis
nucleotide-excision repair, DNA incision
cellular response to UV
DNA damage response, detection of DNA damage
DNA replication proofreading
fatty acid homeostasis
DNA catabolic process, exonucleolytic
DNA-dependent DNA replication
DNA biosynthetic process
nucleic acid phosphodiester bond hydrolysis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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