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POLD1 Antibody (MA5-35464) in WB
Western blot analysis of POLD1 in extracts of various cell lines. Samples were incubated with POLD1 Monoclonal antibody (Product # MA5-35464) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
MA5-35464
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC0930
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1000-1107 of human POLD1 (P28340)
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849365
Product Specific Information
Immunogen sequence: GGLLAFAKRR NCCIGCRTVL SHQGAVCEFC QPRESELYQK EVSHLNALEE RFSRLWTQCQ RCQGSLHEDV ICTSRDCPIF YMRKKVRKDL EDQEQLLRRF GPPGPEAW
Target Information
This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3'-5' exodeoxyribonuclease; CDC2 homolog; DNA polymerase delta 1, catalytic domain; DNA polymerase delta catalytic subunit; DNA polymerase delta, catalytic subunit; DNA polymerase subunit delta p125; DNA-directed DNA polymerase delta 1; POL D; polymerase (DNA directed), delta 1, catalytic subunit 125kDa; polymerase (DNA) delta 1, catalytic subunit; Polymerase D
Gene Aliases: 125kDa; CDC2; CRCS10; MDPL; POLD; POLD1
UniProt ID: (Human) P28340, (Rat) O54747, (Mouse) P52431
Entrez Gene ID: (Human) 5424, (Rat) 59294, (Mouse) 18971
nucleotide binding
DNA binding
chromatin binding
damaged DNA binding
DNA-directed DNA polymerase activity
protein binding
3'-5'-exodeoxyribonuclease activity
enzyme binding
metal ion binding
4 iron, 4 sulfur cluster binding
3'-5' exonuclease activity
nucleic acid binding
nuclease activity
exonuclease activity
transferase activity
nucleotidyltransferase activity
hydrolase activity
iron-sulfur cluster binding
DNA metabolism protein
telomere maintenance via recombination
telomere maintenance
DNA synthesis involved in DNA repair
DNA replication
DNA strand elongation involved in DNA replication
DNA repair
transcription-coupled nucleotide-excision repair
base-excision repair, gap-filling
nucleotide-excision repair, DNA incision, 5'-to lesion
nucleotide-excision repair, DNA gap filling
mismatch repair
response to UV
translesion synthesis
nucleotide-excision repair, DNA incision
cellular response to UV
DNA damage response, detection of DNA damage
DNA replication proofreading
fatty acid homeostasis
DNA catabolic process, exonucleolytic
DNA-dependent DNA replication
DNA biosynthetic process
nucleic acid phosphodiester bond hydrolysis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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