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POLI Polyclonal Antibody

POLI Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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POLI Antibody (13635-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human pancreas cancer using 13635-1-AP (POLI antibody) at dilution of 1:100 (under 40x lens).

POLI Antibody in Immunoprecipitation (IP)

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Product Details

13635-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,200

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

POLI Fusion Protein Ag4563 (1-340 aa encoded by BC032662)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MELADVGAA ASSQGVHDQV LPTPNASSRV IVHVDLDCFY AQVEMISNPE LKDKPLGVQQ KYLVVTCNYE ARKLGVKKLM NVRDAKEKCP QLVLVNGEDL TRYREMSYKV TELLEEFSPV VERLGFDENF VDLTEMVEKR LQQLQSDELS AVTVSGHVYN NQSINLLDVL HIRLLVGSQI AAEMREAMYN QLGLTGCAGV ASNKLLAKLV SGVFKPNQQT VLLPESCQHL IHSLNHIKEI PGIGYKTAKC LEALGINSVR DLQTFSPKIL EKELGISVAQ RIQKLSFGED NSPVILSGPP QSFSEEDSFK KCSSEVEAKN KIEELLASLL NRVCQDGRKP H (1-340 aa encoded by BC032662)

Target Information

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Diverse cellular morphologies during lumen maturation in Anopheles gambiae larval salivary glands.

Insect molecular biology

Chiu M,Trigg B,Taracena M,Wells M

Thu Apr 01 00:00:00 EDT 2021

DNA Polymerase Iota Promotes Esophageal Squamous Cell Carcinoma Proliferation Through Erk-OGT-Induced G6PD Overactivation.

Frontiers in oncology

Su Z,Gao A,Li X,Zou S,He C,Wu J,Ding WQ,Zhou J

Sat Aug 07 00:00:00 EDT 2021

Phosphorylation of ETS-1 is a critical event in DNA polymerase iota-induced invasion and metastasis of esophageal squamous cell carcinoma.

Cancer science

He C,Wu S,Gao A,Su Y,Min H,Shang ZF,Wu J,Yang L,Ding WQ,Zhou J

Fri Dec 01 00:00:00 EST 2017

Phosphorylation of ETS-1 is a critical event in DNA polymerase iota-induced invasion and metastasis of esophageal squamous cell carcinoma.

Cancer science

He C,Wu S,Gao A,Su Y,Min H,Shang ZF,Wu J,Yang L,Ding WQ,Zhou J

Fri Dec 01 00:00:00 EST 2017

Phosphorylation of ETS-1 is a critical event in DNA polymerase iota-induced invasion and metastasis of esophageal squamous cell carcinoma.

Cancer science

He C,Wu S,Gao A,Su Y,Min H,Shang ZF,Wu J,Yang L,Ding WQ,Zhou J

Fri Dec 01 00:00:00 EST 2017

Phosphorylation of ETS-1 is a critical event in DNA polymerase iota-induced invasion and metastasis of esophageal squamous cell carcinoma.

Cancer science

He C,Wu S,Gao A,Su Y,Min H,Shang ZF,Wu J,Yang L,Ding WQ,Zhou J

Fri Dec 01 00:00:00 EST 2017

DNA polymerase iota (Pol ι) promotes invasion and metastasis of esophageal squamous cell carcinoma.

Oncotarget

Zou S,Shang ZF,Liu B,Zhang S,Wu J,Huang M,Ding WQ,Zhou J

Tue May 31 00:00:00 EDT 2016

DNA polymerase iota (Pol ι) promotes invasion and metastasis of esophageal squamous cell carcinoma.

Oncotarget

Zou S,Shang ZF,Liu B,Zhang S,Wu J,Huang M,Ding WQ,Zhou J

Tue May 31 00:00:00 EDT 2016

Elevated DNA polymerase iota (Poli) is involved in the acquisition of aggressive phenotypes of human esophageal squamous cell cancer.

International journal of clinical and experimental pathology

Sun H,Zou S,Zhang S,Liu B,Meng X,Li X,Yu J,Wu J,Zhou J

Mon Apr 17 00:00:00 EDT 2017

Elevated DNA polymerase iota (Poli) is involved in the acquisition of aggressive phenotypes of human esophageal squamous cell cancer.

International journal of clinical and experimental pathology

Sun H,Zou S,Zhang S,Liu B,Meng X,Li X,Yu J,Wu J,Zhou J

Mon Apr 17 00:00:00 EDT 2017

Elevated DNA polymerase iota (Poli) is involved in the acquisition of aggressive phenotypes of human esophageal squamous cell cancer.

International journal of clinical and experimental pathology

Sun H,Zou S,Zhang S,Liu B,Meng X,Li X,Yu J,Wu J,Zhou J

Mon Apr 17 00:00:00 EDT 2017

The error-prone DNA polymerase ι provides quantitative resistance to lung tumorigenesis and mutagenesis in mice.

Oncogene

Iguchi M,Osanai M,Hayashi Y,Koentgen F,Lee GH

Thu Jul 03 00:00:00 EDT 2014

The error-prone DNA polymerase ι provides quantitative resistance to lung tumorigenesis and mutagenesis in mice.

Oncogene

Iguchi M,Osanai M,Hayashi Y,Koentgen F,Lee GH

Thu Jul 03 00:00:00 EDT 2014

Transient expression and activity of human DNA polymerase iota in loach embryos.

Biotechnology letters

Makarova IV,Kazakov AA,Makarova AV,Khaidarova NV,Kozikova LV,Nenasheva VV,Gening LV,Tarantul VZ,Andreeva LE

Wed Feb 01 00:00:00 EST 2012

Transient expression and activity of human DNA polymerase iota in loach embryos.

Biotechnology letters

Makarova IV,Kazakov AA,Makarova AV,Khaidarova NV,Kozikova LV,Nenasheva VV,Gening LV,Tarantul VZ,Andreeva LE

Wed Feb 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: DNA polymerase iota; Eta2; polymerase (DNA directed) iota; polymerase (DNA) iota; polymerase (DNA-directed), iota; RAD 30B; RAD30; RAD30 homolog B

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Gene Aliases: POLI; RAD30B; RAD3OB

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UniProt ID: (Human) Q9UNA4, (Mouse) Q6R3M4

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Entrez Gene ID: (Human) 11201, (Mouse) 26447, (Rat) 291526

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Function(s)

damaged DNA binding DNA-directed DNA polymerase activity protein binding metal ion binding DNA binding transferase activity nucleotidyltransferase activity DNA metabolism protein nucleic acid metabolism protein

Process(es)

DNA replication DNA repair translesion synthesis error-prone translesion synthesis cellular response to DNA damage stimulus cellular response to UV-C DNA biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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POLI Polyclonal Antibody

POLI Antibody (13635-1-AP) in IHC (P)

Product Details

13635-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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