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POLQ Antibody (PA5-115130) in ICC/IF
Immunocytochemistry analysis of POLQ in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115130). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Product Details
PA5-115130
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human POLQ(Accession O75417), corresponding to amino acid residues K1581-W1631.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2899766
Product Specific Information
Antibody detects endogenous levels of total DNA Polymerase theta.
Target Information
DNA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA (PubMed:25642963, PubMed:25643323). MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation (PubMed:25642963, PubMed:25643323). POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed:25642963). POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions. The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ (PubMed:25643323). The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates (PubMed:18503084, PubMed:21050863, PubMed:22135286). Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining (PubMed:14576298, PubMed:18503084, PubMed:19188258, PubMed:24648516). Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Chromosome aberrations occurring spontaneously protein 1; DNA polymerase eta; DNA polymerase theta; EC 2.7.7.7; polymerase (DNA directed), theta; polymerase (DNA) theta
Gene Aliases: A430110D14Rik; Chaos1; POLH; POLQ; PRO0327
UniProt ID: (Human) O75417, (Mouse) Q8CGS6
Entrez Gene ID: (Human) 10721, (Rat) 288079, (Mouse) 77782
chromatin binding
damaged DNA binding
DNA-directed DNA polymerase activity
protein binding
ATP binding
single-stranded DNA-dependent ATPase activity
5'-deoxyribose-5-phosphate lyase activity
DNA binding
nucleotide binding
nucleic acid binding
transferase activity
nucleotidyltransferase activity
DNA-directed DNA polymerase
double-strand break repair via homologous recombination
DNA-dependent DNA replication
DNA repair
base-excision repair
double-strand break repair
cellular response to DNA damage stimulus
somatic hypermutation of immunoglobulin genes
protein homooligomerization
DNA biosynthetic process
double-strand break repair via alternative nonhomologous end joining
negative regulation of double-strand break repair via homologous recombination
DNA replication
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