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POU3F2 Antibody (PA5-23400) in WB
Western blot analysis of POU3F2 in human Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) mouse NIH 3T3 cell lysate in absence of immunizing peptide. Samples were incubated in POU3F2 polyclonal antibody (Product # PA5-23400) followed by a goat anti-rabbit Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
Product Details
PA5-23400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Horse,
Human,
Mouse,
Non-human primate,
Rhesus monkey,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A portion of amino acids 240 to 290 of human POU3F2
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2540923
Product Specific Information
PA5-23400 targets POU3F2 in IHC (P) and WB applications and shows reactivity with Bovine, Canine, Equine, Human, mouse, and Rat samples.
The PA5-23400 immunogen is synthetic peptide (254 AHHDPHSDEDTPTSDD 269) of POU3F2 protein.
Target Information
This gene encodes a member of the POU-III class of neural transcription factors. The encoded protein is involved in neuronal differentiation and enhances the activation of corticotropin-releasing hormone regulated genes. Overexpression of this protein is associated with an increase in the proliferation of melanoma cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 7-Oct; Brain-2; brain-2 class III POU-domain protein; Brain-specific homeobox/POU domain protein 2; Nervous system-specific octamer-binding transcription factor N-Oct-3; Oct-7; Octamer-binding protein 7; Octamer-binding transcription factor 7; OTF-7; POU domain, class 3, transcription factor 2; protein Brn-2
Gene Aliases: 9430075J19Rik; A230098E07Rik; Brn-2; BRN2; N-Oct3; oct-7; OCT7; OTF-7; OTF7; POU3F2; POUF3
UniProt ID: (Human) Q14960, (Mouse) P31360
Entrez Gene ID: (Human) 5454, (Rhesus monkey) 694524, (Dog) 481931, (Bovine) 783320, (Horse) 100071557, (Mouse) 18992, (Rat) 29588
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription coactivator activity
transcription factor activity, sequence-specific DNA binding
protein binding
identical protein binding
DNA binding
sequence-specific DNA binding
HMG box domain binding
transcription from RNA polymerase II promoter
positive regulation of cell proliferation
epidermis development
negative regulation of gene expression
astrocyte development
cerebral cortex radially oriented cell migration
forebrain ventricular zone progenitor cell division
hypothalamus cell differentiation
neurohypophysis development
myelination in peripheral nervous system
neuron differentiation
positive regulation of multicellular organism growth
positive regulation of transcription from RNA polymerase II promoter
regulation of axonogenesis
cellular response to organic substance
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
Schwann cell development
regulation of cell differentiation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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