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Invitrogen
PP2A alpha Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 10
PP2A alpha Antibody (PA5-29754) in ICC/IF
Immunofluorescence analysis of PP2A alpha was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PP2A alpha Polyclonal Antibody (Product # PA5-29754) at 1:500 dilution in 0.1% BSA, incubated at 4 degree celsiu... View More
Product Details
PA5-29754
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1 and 231 of Human PP2A alpha
Conjugate
Form
Liquid
Concentration
0.45 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547228
Product Specific Information
Recommended positive controls: HCT116, HeLa, HepG2, Molt-4, mouse brain.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (97%), Xenopus laevis (100%), Dog (99%), Pig (100%), Rabbit (100%), Chicken (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. The PP2A family comprises subfamily members PP2A alpha and PP2A beta. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B55-GAMMA; IMYPNO; Methyl-PPP2CA; MGC33570; PP2A alpha; PP2A C terminal; PP2A L9; PP2A Leucine 9; PP2A-alpha; PR52; PR55G; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2a, catalytic subunit, alpha isoform; Replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
Gene Aliases: PP2A; PP2Ac; PP2CA; PP2Calpha; PPP2CA; R75353; RP-C
UniProt ID: (Human) P67775, (Mouse) P63330
Entrez Gene ID: (Human) 5515, (Mouse) 19052
phosphoprotein phosphatase activity
protein binding
protein C-terminus binding
metal ion binding
GABA receptor binding
protein serine/threonine phosphatase activity
hydrolase activity
enzyme binding
protein kinase binding
protein phosphatase binding
protein domain specific binding
protein complex binding
protein kinase B binding
ion channel binding
protein dimerization activity
tau protein binding
protein phosphatase 2A binding
protein antigen binding
protein phosphatase
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
inactivation of MAPK activity
regulation of DNA replication
regulation of transcription, DNA-templated
protein dephosphorylation
ceramide metabolic process
apoptotic process
mitotic nuclear envelope reassembly
mesoderm development
RNA splicing
response to organic substance
negative regulation of epithelial to mesenchymal transition
second-messenger-mediated signaling
regulation of Wnt signaling pathway
regulation of cell adhesion
negative regulation of cell growth
regulation of growth
negative regulation of tyrosine phosphorylation of Stat3 protein
regulation of cell differentiation
meiotic cell cycle
positive regulation of protein serine/threonine kinase activity
negative regulation of transcription from RNA polymerase II promoter
regulation of protein phosphorylation
negative regulation of protein phosphorylation
regulation of receptor activity
regulation of protein autophosphorylation
positive regulation of protein phosphatase type 2A activity
positive regulation of protein dephosphorylation
regulation of protein catabolic process
negative regulation of transcription factor import into nucleus
positive regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of cell cycle
protein heterotrimerization
cellular response to glucose stimulus
negative regulation of calcium ion transmembrane transporter activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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