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Invitrogen
PPA1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
PPA1 Antibody (PA5-30125) in WB
Western blot was performed using Anti-PPA1 Rabbit Polyclonal Antibody (Product # PA5-30125) and 32 kDa band corresponding to PPA1 was observed across the samples tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), U-87 MG (Lane 2), HT-29 (Lane 3),HCT 116 (Lane 4), SW-480 (Lane 5),MCF-7 (Lane 6) and tissue extract of Mouse Small Intestine (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 ... View More
Product Details
PA5-30125
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 92 and 289 of Human PPA1
Conjugate
Form
Liquid
Concentration
0.95 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547599
Product Specific Information
Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7.
Predicted reactivity: Mouse (92%), Rat (90%), Bovine (93%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytosolic inorganic pyrophosphatase; diphosphate phosphohydrolase; epididymis secretory sperm binding protein Li 66p; inorganic diphosphatase; inorganic diphosphatase 1; Inorganic pyrophosphatase; inorganic pyrophosphatase 1; PPase; pyrophosphatase 1; Pyrophosphate phospho-hydrolase; RP11-367H5.1
Gene Aliases: 2010317E03Rik; HEL-S-66p; IOPPP; PP; PP1; PPA1; Pyp; SID6-8061
UniProt ID: (Human) Q15181, (Mouse) Q9D819
Entrez Gene ID: (Human) 5464, (Mouse) 67895
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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