Invitrogen

PPAR alpha Monoclonal Antibody (3B6/PPAR)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

12 Published Figures

21 References

View all (34) PPAR alpha antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

PPAR alpha Antibody in ChIP Assay (ChIP)

FIGURE: 1 / 17

PPAR alpha Antibody (MA1-822) in ChIP

Hepatology communications 2020 - 5 FIG. Malnutrition is associated with decreased FXR binding but not increased PPARalpha binding. (A) PPARalpha-ChIP-qPCR reveals no increased binding on the known PPARalpha targets Fgf21 , Acox1 , or Cyp4a14 nor on Fga or F11 , in mice fed the LPLFD (n = 3-4). This is in contrast to increased PPARalpha binding on all five genes in mice following a 24-hour fast (Supporting Fig. S6). (B) FXR-ChIP-qPCR reveal... View More

PPAR alpha Antibody in Western Blot (WB)

PPAR alpha Antibody in Flow Cytometry (Flow)

PPAR alpha Antibody in Western Blot, Immunohistochemistry (WB, IHC)

PPAR alpha Antibody in Immunohistochemistry (IHC)

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Product Details

MA1-822

Applications

Tested Dilution

Publications

Western Blot (WB)

2-5 µg/mL

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

- -

Flow Cytometry (Flow)

1 µg / 10^6 cells

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

ChIP assay (ChIP)

Assay-dependent

View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bovine, Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

3B6/PPAR

Immunogen

Purified recombinant PPAR alpha protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2165745

Product Specific Information

MA1-822 detects peroxisome proliferator activated receptors (PPAR) alpha from human, mouse, and rat tissues.

MA1-822 has been successfully used in Western blot, Gel Shift and immunoprecipitation procedures. By Western blot, this antibody detects a ~52 kDa protein which corresponds to PPAR alpha from mouse adipose tissue extract. This antibody detects some non-specific bands on NIH-3T3 cell lysates.

The MA1-822 immunogen is purified recombinant PPAR alpha protein.

Target Information

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR's). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR's are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR's can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Diets Differently Regulate Pulmonary Pathogenesis and Immune Signaling in Mice during Acute and Chronic Mycobacterium tuberculosis Infection.

Life (Basel, Switzerland)

Oswal N,Thangavel H,Lizardo K,Dhanyalayam D,Sidrat T,Salgame P,Nagajyothi JF

Published figure using PPAR alpha monoclonal antibody (Product # MA1-822) in Western Blot

Fri Jan 13 00:00:00 EST 2023

Diets Differently Regulate Pulmonary Pathogenesis and Immune Signaling in Mice during Acute and Chronic Mycobacterium tuberculosis Infection.

Life (Basel, Switzerland)

Oswal N,Thangavel H,Lizardo K,Dhanyalayam D,Sidrat T,Salgame P,Nagajyothi JF

Published figure using PPAR alpha monoclonal antibody (Product # MA1-822) in Western Blot

Fri Jan 13 00:00:00 EST 2023

Host Metabolic Changes during Mycobacterium Tuberculosis Infection Cause Insulin Resistance in Adult Mice.

Journal of clinical medicine

Oswal N,Lizardo K,Dhanyalayam D,Ayyappan JP,Thangavel H,Heysell SK,Nagajyothi JF

Published figure using PPAR alpha monoclonal antibody (Product # MA1-822) in Western Blot

Wed Mar 16 00:00:00 EDT 2022

Modulatory Effects of Estradiol and Its Mixtures with Ligands of GPER and PPAR on MAPK and PI3K/Akt Signaling Pathways and Tumorigenic Factors in Mouse Testis Explants and Mouse Tumor Leydig Cells.

Biomedicines

Gorowska-Wojtowicz E,Duliban M,Kotula-Balak M,Bilinska B

MA1-822 was used in Western Blotting to suggest that a balanced estrogen level and its action together with proper GPER and PPAR signaling play a key role in the maintenance of testis homeostasis.

Sun Jun 12 00:00:00 EDT 2022

Modulatory Effects of Estradiol and Its Mixtures with Ligands of GPER and PPAR on MAPK and PI3K/Akt Signaling Pathways and Tumorigenic Factors in Mouse Testis Explants and Mouse Tumor Leydig Cells.

Biomedicines

Gorowska-Wojtowicz E,Duliban M,Kotula-Balak M,Bilinska B

MA1-822 was used in Western Blotting to suggest that a balanced estrogen level and its action together with proper GPER and PPAR signaling play a key role in the maintenance of testis homeostasis.

Sun Jun 12 00:00:00 EDT 2022

SIRT1 promotes lipid metabolism and mitochondrial biogenesis in adipocytes and coordinates adipogenesis by targeting key enzymatic pathways.

Scientific reports

Majeed Y,Halabi N,Madani AY,Engelke R,Bhagwat AM,Abdesselem H,Agha MV,Vakayil M,Courjaret R,Goswami N,Hamidane HB,Elrayess MA,Rafii A,Graumann J,Schmidt F,Mazloum NA

MA1-822 was used in Western Blotting to characterize SIRT1-depleted adipocytes by quantitative mass spectrometry-based proteomics, gene-expression and biochemical analyses, and mitochondrial studies.

Wed Apr 14 00:00:00 EDT 2021

SIRT1 promotes lipid metabolism and mitochondrial biogenesis in adipocytes and coordinates adipogenesis by targeting key enzymatic pathways.

Scientific reports

Majeed Y,Halabi N,Madani AY,Engelke R,Bhagwat AM,Abdesselem H,Agha MV,Vakayil M,Courjaret R,Goswami N,Hamidane HB,Elrayess MA,Rafii A,Graumann J,Schmidt F,Mazloum NA

MA1-822 was used in Western Blotting to characterize SIRT1-depleted adipocytes by quantitative mass spectrometry-based proteomics, gene-expression and biochemical analyses, and mitochondrial studies.

Wed Apr 14 00:00:00 EDT 2021

Fat tissue regulates the pathogenesis and severity of cardiomyopathy in murine chagas disease.

PLoS neglected tropical diseases

Lizardo K,Ayyappan JP,Oswal N,Weiss LM,Scherer PE,Nagajyothi JF

Published figure using PPAR alpha monoclonal antibody (Product # MA1-822) in Western Blot

Thu Apr 01 00:00:00 EDT 2021

Noise-Induced Cochlear Damage Involves PPAR Down-Regulation through the Interplay between Oxidative Stress and Inflammation.

Antioxidants (Basel, Switzerland)

Paciello F,Pisani A,Rolesi R,Escarrat V,Galli J,Paludetti G,Grassi C,Troiani D,Fetoni AR

MA1-822 was used in Immunohistochemistry (Frozen), Western Blot to investigate how oxidative stress and inflammation participate in cochlear dysfunction through PPAR signaling pathways.

Mon Jul 26 00:00:00 EDT 2021

Noise-Induced Cochlear Damage Involves PPAR Down-Regulation through the Interplay between Oxidative Stress and Inflammation.

Antioxidants (Basel, Switzerland)

Paciello F,Pisani A,Rolesi R,Escarrat V,Galli J,Paludetti G,Grassi C,Troiani D,Fetoni AR

MA1-822 was used in Immunohistochemistry (Frozen), Western Blot to investigate how oxidative stress and inflammation participate in cochlear dysfunction through PPAR signaling pathways.

Mon Jul 26 00:00:00 EDT 2021

Noise-Induced Cochlear Damage Involves PPAR Down-Regulation through the Interplay between Oxidative Stress and Inflammation.

Antioxidants (Basel, Switzerland)

Paciello F,Pisani A,Rolesi R,Escarrat V,Galli J,Paludetti G,Grassi C,Troiani D,Fetoni AR

MA1-822 was used in Immunohistochemistry (Frozen), Western Blot to investigate how oxidative stress and inflammation participate in cochlear dysfunction through PPAR signaling pathways.

Mon Jul 26 00:00:00 EDT 2021

Protective Effects of Bariatric Surgery on Kidney Functions by Inhibiting Oxidative Stress Responses Through Activating PPARα in Rats With Diabetes.

Frontiers in physiology

Jiang HW,Zhou Y,Zhou PY,Zhang TY,Hu JY,Bai XT

MA1-822 was used in Immunohistochemistry to study the protective effects and the regulatory mechanisms of bariatric surgery on kidney injury in diabetic rats.

Sun Apr 24 00:00:00 EDT 2022

Protective Effects of Bariatric Surgery on Kidney Functions by Inhibiting Oxidative Stress Responses Through Activating PPARα in Rats With Diabetes.

Frontiers in physiology

Jiang HW,Zhou Y,Zhou PY,Zhang TY,Hu JY,Bai XT

MA1-822 was used in Immunohistochemistry to study the protective effects and the regulatory mechanisms of bariatric surgery on kidney injury in diabetic rats.

Sun Apr 24 00:00:00 EDT 2022

PPARα exacerbates necroptosis, leading to increased mortality in postinfluenza bacterial superinfection.

Proceedings of the National Academy of Sciences of the United States of America

Tam VC,Suen R,Treuting PM,Armando A,Lucarelli R,Gorrochotegui-Escalante N,Diercks AH,Quehenberger O,Dennis EA,Aderem A,Gold ES

MA1-822 was used in Western Blotting to identify a molecular circuit in which infection with influenza induces CYP450 metabolites that activate PPARα, leading to increased necrotic cell death in the lung which correlates with the excess mortality observed in superinfection.

Tue Jul 07 00:00:00 EDT 2020

Towards understanding leydigioma: do G protein-coupled estrogen receptor and peroxisome proliferator-activated receptor regulate lipid metabolism and steroidogenesis in Leydig cell tumors?

Protoplasma

Kotula-Balak M,Gorowska-Wojtowicz E,Milon A,Pawlicki P,Tworzydlo W,Płachno BJ,Krakowska I,Hejmej A,Wolski JK,Bilinska B

MA1-822 was used in Western Blot, Immunohistochemistry to investigate the G protein-coupled estrogen receptor (GPER) and peroxisome proliferator-activated receptor (PPAR) implication in regulation of lipid homeostasis including the expression of steroidogenesis-controlling molecules in clinical specimens of LCTs and tumor Leydig cells (MA-10).

Wed Jul 01 00:00:00 EDT 2020

Towards understanding leydigioma: do G protein-coupled estrogen receptor and peroxisome proliferator-activated receptor regulate lipid metabolism and steroidogenesis in Leydig cell tumors?

Protoplasma

Kotula-Balak M,Gorowska-Wojtowicz E,Milon A,Pawlicki P,Tworzydlo W,Płachno BJ,Krakowska I,Hejmej A,Wolski JK,Bilinska B

Wed Jul 01 00:00:00 EDT 2020

Coagulopathy in Malnourished Mice Is Sexually Dimorphic and Regulated by Nutrient-Sensing Nuclear Receptors.

Hepatology communications

Preidis GA,Soni KG,Suh JH,Halder T,Kim KH,Choi JM,Li F,Devaraj S,Conner ME,Coarfa C,Jung SY,Moore DD

MA1-822 was used in Chromatin immunoprecipitation to show that malnutrition impairs growth and liver synthetic function more severely in male mice than in female mice.

Tue Dec 01 00:00:00 EST 2020

Coagulopathy in Malnourished Mice Is Sexually Dimorphic and Regulated by Nutrient-Sensing Nuclear Receptors.

Hepatology communications

Preidis GA,Soni KG,Suh JH,Halder T,Kim KH,Choi JM,Li F,Devaraj S,Conner ME,Coarfa C,Jung SY,Moore DD

MA1-822 was used in Chromatin immunoprecipitation to show that malnutrition impairs growth and liver synthetic function more severely in male mice than in female mice.

Tue Dec 01 00:00:00 EST 2020

Inhibition of SREBP Improves Cardiac Lipidopathy, Improves Endoplasmic Reticulum Stress, and Modulates Chronic Chagas Cardiomyopathy.

Journal of the American Heart Association

Ayyappan JP,Lizardo K,Wang S,Yurkow E,Nagajyothi JF

Published figure using PPAR alpha monoclonal antibody (Product # MA1-822) in Western Blot

Tue Feb 04 00:00:00 EST 2020

Adipose Tissue Regulates Pulmonary Pathology during TB Infection.

mBio

Ayyappan JP,Ganapathi U,Lizardo K,Vinnard C,Subbian S,Perlin DS,Nagajyothi JF

MA1-822 was used in Western Blotting to investigate the association between Mycobacterium tuberculosis infection, adipose tissue, and tuberculosis disease progression.

Tue Apr 16 00:00:00 EDT 2019

Ameliorative effects of pepsin-digested chicken liver hydrolysates on development of alcoholic fatty livers in mice.

Food & function

Lin YL,Tai SY,Chen JW,Chou CH,Fu SG,Chen YC

MA1822 was used in western blot to determine the protective effects of pepsin-digested chicken liver hydrolysates against chronic alcohol consumption

Wed May 24 00:00:00 EDT 2017

Selective reversible inhibition of liver carnitine palmitoyl-transferase 1 by teglicar reduces gluconeogenesis and improves glucose homeostasis.

Diabetes

Conti R,Mannucci E,Pessotto P,Tassoni E,Carminati P,Giannessi F,Arduini A

MA1-822 was used in western blot to investigate the effect of liver carnitine palmitoyl-transferase 1 inhibition on gluconeogenesis and glucose homeostasis

Tue Feb 01 00:00:00 EST 2011

Peroxisome proliferator-activated receptor alpha physically interacts with CCAAT/enhancer binding protein (C/EBPbeta) to inhibit C/EBPbeta-responsive alpha1-acid glycoprotein gene expression.

Molecular endocrinology (Baltimore, Md.)

Mouthiers A,Baillet A,Deloménie C,Porquet D,Mejdoubi-Charef N

MA1-822 was used in immunoprecipitation to study the interaction between peroxisome proliferator-activated receptor alpha and CCAAT/enhancer binding protein (C/EBPbeta) and its effect on alpha1 acid glycoprotein gene expression.

Sun May 01 00:00:00 EDT 2005

Noradrenaline represses PPAR (peroxisome-proliferator-activated receptor) gamma2 gene expression in brown adipocytes: intracellular signalling and effects on PPARgamma2 and PPARgamma1 protein levels.

The Biochemical journal

Lindgren EM,Nielsen R,Petrovic N,Jacobsson A,Mandrup S,Cannon B,Nedergaard J

MA1-822 was used in western blot to investigate the effect of noradrenaline on PPAR (peroxisome-proliferator-activated receptor)-gamma gene expression in brown adipocytes

Wed Sep 01 00:00:00 EDT 2004

Peroxisome proliferator-activated receptor alpha is downregulated in the failing human heart.

Cellular & molecular biology letters

Karbowska J,Kochan Z,Smoleński RT

MA1-822 was used in western blot to investigate the role of peroxisome proliferator-activated receptor alpha in the failing human heart

Mon Jan 05 00:00:00 EST 2004

Evidence that peroxisome proliferator-activated receptor alpha is complexed with the 90-kDa heat shock protein and the hepatitis virus B X-associated protein 2.

The Journal of biological chemistry

Sumanasekera WK,Tien ES,Turpey R,Vanden Heuvel JP,Perdew GH

MA1-822 was used in immunoprecipitation to investigate the interaction of PPARalpha with hsp90 and XAP2 and the function of XAP2.

Fri Feb 14 00:00:00 EST 2003

Effect of stages of lactation on the concentration of a 90-kilodalton heat shock protein in bovine mammary tissue.

Journal of dairy science

Watanabe A,Miyamoto T,Katoh N,Takahashi Y

MA1-822 was used in western blot to investigate the differences of HSP90 expression in cow mammary tissue at various lactation stages

Wed Oct 01 00:00:00 EDT 1997

Bioinformatics

Protein Aliases: MGC2237; MGC2452; Nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator-activated nuclear receptor alpha variant 3; Peroxisome proliferator-activated receptor alpha; PPAR-alpha

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Gene Aliases: 4933429D07Rik; AW742785; hPPAR; NR1C1; PPAR; PPAR-alpha; PPARA; PPARalpha

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UniProt ID: (Human) Q07869, (Rat) P37230, (Mouse) P23204

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Entrez Gene ID: (Human) 5465, (Rat) 25747, (Mouse) 19013

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II repressing transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription coactivator binding DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding transcription factor binding drug binding zinc ion binding lipid binding phosphatase binding protein domain specific binding ubiquitin conjugating enzyme binding protein complex binding sequence-specific DNA binding NFAT protein binding MDM2/MDM4 family protein binding receptor activity metal ion binding protein heterodimerization activity C4 zinc finger nuclear receptor DNA-binding transcription factor zinc finger transcription factor gene-specific transcriptional regulator

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PPAR alpha Monoclonal Antibody (3B6/PPAR)

PPAR alpha Antibody (MA1-822) in ChIP

Product Details

MA1-822

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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