Invitrogen

PPAR gamma-2 Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

4 Published Figures

19 References

View all (2) PPAR gamma-2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

PPAR gamma-2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

PPAR gamma-2 Antibody (PA1-824) in ICC/IF

Immunofluorescent analysis of PPAR gamma (red) in 3T3-L1 differentiated day 7 cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a PPAR gamma rabbit polyclonal antibody (Product # PA1-824) at a dilution of 1:200 in blocking buff... View More

PPAR gamma-2 Antibody in Western Blot (WB)

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Product Details

PA1-824

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:500

View 4 publications 4 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Gel Shift (GS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) G E T L G D S P I D P E S D S(16) C of human PPAR gamma-2.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2166200

Product Specific Information

PA1-824 detects peroxisome proliferator activated receptor (PPAR) gamma 2 from human and mouse tissues. This antibody does not detect PPAR alpha or PPAR delta.

PA1-824 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~56 kDa protein representing PPAR gamma 2 from NIH-3T3 cell lysate. PA1-824 inhibits PPAR gamma 2 DNA binding.

PA1-824 immunogen is a synthetic peptide corresponding to residues M(1) G E T L G D S P I D P E S D S(16) C of human PPAR gamma-2. This sequence is not conserved in PPAR gamma-1.

PA1-824 can be used with blocking peptide PEP-027.

Target Information

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR gamma 2, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Dimensionality changes actin network through lamin A/C and zyxin.

Biomaterials

Zonderland J,Moldero IL,Anand S,Mota C,Moroni L

PA1-824 was used in Immunocytochemistry to assess the role of the actin cytoskeleton in regulating 3D tissue engineered scaffolds made from human mesenchymal stromal cells.

Fri May 01 00:00:00 EDT 2020

Soya protein β-conglycinin ameliorates fatty liver and obesity in diet-induced obese mice through the down-regulation of PPARγ.

The British journal of nutrition

Li D,Ikaga R,Yamazaki T

PA1-824 was used in Western Blotting to investigate the effects of β-conglycinin on the improvement of obesity and NAFLD in high-fat (HF) diet-induced obese (DIO) mice.

Fri Jun 01 00:00:00 EDT 2018

A truncated PPAR gamma 2 localizes to mitochondria and regulates mitochondrial respiration in brown adipocytes.

PloS one

Chang JS,Ha K

Published figure using PPAR gamma-2 polyclonal antibody (Product # PA1-824) in Western Blot

Fri Jul 27 00:00:00 EDT 2018

Peroxisome Proliferator-Activated Receptor α Facilitates Osteogenic Differentiation in MC3T3-E1 Cells via the Sirtuin 1-Dependent Signaling Pathway.

Molecules and cells

Gong K,Qu B,Wang C,Zhou J,Liao D,Zheng W,Pan X

PA1-824 was used in Western Blotting to indicate that PPARα promotes osteogenic differentiation via the Sirt1-dependent signalling pathway.

Fri Jun 30 00:00:00 EDT 2017

Databases for technical aspects of immunohistochemistry.

Journal of toxicologic pathology

Furukawa S,Nagaike M,Ozaki K

PA1-824 was used in Immunohistochemistry to gather responses to a questionnaire gathering information on suitable choices of antibody for histopathological examination.

Sun Jan 01 00:00:00 EST 2017

Sirtuin1 promotes osteogenic differentiation through downregulation of peroxisome proliferator-activated receptor γ in MC3T3-E1 cells.

Biochemical and biophysical research communications

Qu B,Ma Y,Yan M,Gong K,Liang F,Deng S,Jiang K,Ma Z,Pan X

PA1-824 was used in immunoprecipitation and western blot to investigate the molecular mechanism of Sirt1 in osteogenic differentiation

Fri Sep 09 00:00:00 EDT 2016

Effect of retinoic acid and vitamin D3 on osteoblast differentiation and activity in aging.

Journal of bone and mineral metabolism

Bosetti M,Sabbatini M,Calarco A,Borrone A,Peluso G,Cannas M

PA1-824 was used in immunocytochemistry to study the age-dependent effects of retinoic acid and calcitriol on PPARgamma2 signaling in osteoblast

Fri Jan 01 00:00:00 EST 2016

Lipid-Laden Multilocular Cells in the Aging Thymus Are Phenotypically Heterogeneous.

PloS one

Langhi LG,Andrade LR,Shimabukuro MK,van Ewijk W,Taub DD,Borojevic R,de Mello Coelho V

PA1-824 was used in immunohistochemistry - frozen section to characterize intrathymic lipid-laden multilocular cells in the aging thymus

Thu Jun 16 00:00:00 EDT 2016

Dietary β-conglycinin prevents fatty liver induced by a high-fat diet by a decrease in peroxisome proliferator-activated receptor γ2 protein.

The Journal of nutritional biochemistry

Yamazaki T,Kishimoto K,Miura S,Ezaki O

PA1-824 was used in western blot to study the mechanism for the effect of dietary conglycinin on hepatic lipogenesis

Wed Feb 01 00:00:00 EST 2012

An increase in liver PPARγ2 is an initial event to induce fatty liver in response to a diet high in butter: PPARγ2 knockdown improves fatty liver induced by high-saturated fat.

The Journal of nutritional biochemistry

Yamazaki T,Shiraishi S,Kishimoto K,Miura S,Ezaki O

PA1-824 was used in western blot to investigate the role of PPARgamma2 on fat liver formation mediated by high-saturated fat

Wed Jun 01 00:00:00 EDT 2011

Signaling and biological effects of glucagon-like peptide 1 on the differentiation of mesenchymal stem cells from human bone marrow.

American journal of physiology. Endocrinology and metabolism

Sanz C,Vázquez P,Blázquez C,Barrio PA,Alvarez Mdel M,Blázquez E

PA1-824 was used in western blot to investigate the influence of glucagon-like peptide 1 on the differentiation of mesenchymal stem cells

Mon Mar 01 00:00:00 EST 2010

Fat-storing multilocular cells expressing CCR5 increase in the thymus with advancing age: potential role for CCR5 ligands on the differentiation and migration of preadipocytes.

International journal of medical sciences

Mello Coelho Vd,Bunbury A,Rangel LB,Giri B,Weeraratna A,Morin PJ,Bernier M,Taub DD

PA1-824 was used in western blot to characterize receptor activity during fat-storage in the thymus

Fri Dec 04 00:00:00 EST 2009

Perivascular cells expressing annexin A5 define a novel mesenchymal stem cell-like population with the capacity to differentiate into multiple mesenchymal lineages.

Development (Cambridge, England)

Brachvogel B,Moch H,Pausch F,Schlötzer-Schrehardt U,Hofmann C,Hallmann R,von der Mark K,Winkler T,Pöschl E

PA1-824 was used in immunohistochemistry to evaluate the annexin A5 as a marker for multipotent stem cell population

Wed Jun 01 00:00:00 EDT 2005

A human peripheral blood monocyte-derived subset acts as pluripotent stem cells.

Proceedings of the National Academy of Sciences of the United States of America

Zhao Y,Glesne D,Huberman E

PA1-824 was used in immunocytochemistry To investigate the pluripotency of the monocyte derived from human peripheral blood

Tue Mar 04 00:00:00 EST 2003

ADAM 12 protease induces adipogenesis in transgenic mice.

The American journal of pathology

Kawaguchi N,Xu X,Tajima R,Kronqvist P,Sundberg C,Loechel F,Albrechtsen R,Wewer UM

PA1-824 was used in immunohistochemistry to study the role of ADAM 12 protease in adipogenesis.

Wed May 01 00:00:00 EDT 2002

Overexpression and ribozyme-mediated targeting of transcriptional coactivators CREB-binding protein and p300 revealed their indispensable roles in adipocyte differentiation through the regulation of peroxisome proliferator-activated receptor gamma.

The Journal of biological chemistry

Takahashi N,Kawada T,Yamamoto T,Goto T,Taimatsu A,Aoki N,Kawasaki H,Taira K,Yokoyama KK,Kamei Y,Fushiki T

PA1-824 was used in western blot to investigate the role of CBP and p300 in the activation of PPARgama and the adipocyte differentiation.

Fri May 10 00:00:00 EDT 2002

Platelet-derived growth factor promotes the expression of peroxisome proliferator-activated receptor gamma in vascular smooth muscle cells by a phosphatidylinositol 3-kinase/Akt signaling pathway.

Circulation research

Fu M,Zhu X,Wang Q,Zhang J,Song Q,Zheng H,Ogawa W,Du J,Chen YE

PA1-824 was used in western blot to investigate the role of platelet-derived growth factor on the expression of PPARgamma in vascular smooth muscle cells

Fri Nov 23 00:00:00 EST 2001

Diverse signaling pathways modulate nuclear receptor recruitment of N-CoR and SMRT complexes.

Proceedings of the National Academy of Sciences of the United States of America

Lavinsky RM,Jepsen K,Heinzel T,Torchia J,Mullen TM,Schiff R,Del-Rio AL,Ricote M,Ngo S,Gemsch J,Hilsenbeck SG,Osborne CK,Glass CK,Rosenfeld MG,Rose DW

PA1-824 was used in western blot to study the modulatory mechanism for the nuclear receptor recruitment of N-CoR and SMRT complexes

Tue Mar 17 00:00:00 EST 1998

MCF-7 and T47D human breast cancer cells contain a functional peroxisomal response.

Molecular and cellular endocrinology

Kilgore MW,Tate PL,Rai S,Sengoku E,Price TM

PA1-824 was used in EMSA assay to characterize MCF-7 and T47D human breast cancer cells in terms of peroxisomal response

Fri May 16 00:00:00 EDT 1997

Bioinformatics

Protein Aliases: Nuclear receptor subfamily 1 group C member 3; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator activated receptor gamma 2; peroxisome proliferator activated receptor gamma 4; peroxisome proliferator-activated nuclear receptor gamma variant 1; Peroxisome proliferator-activated receptor gamma; PPAR-gamma

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Gene Aliases: CIMT1; GLM1; NR1C3; PPAR-gamma; PPAR-gamma2; PPARG; PPARG1; PPARG2; PPARgamma; PPARgamma2

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UniProt ID: (Human) P37231, (Mouse) P37238

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Entrez Gene ID: (Human) 5468, (Mouse) 19016

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Function(s)

core promoter sequence-specific DNA binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding prostaglandin receptor activity protein binding transcription factor binding drug binding zinc ion binding enzyme binding protein phosphatase binding estrogen receptor binding ligand-dependent nuclear receptor transcription coactivator activity activating transcription factor binding identical protein binding sequence-specific DNA binding transcription regulatory region DNA binding retinoid X receptor binding arachidonic acid binding alpha-actinin binding RNA polymerase II regulatory region DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding metal ion binding WW domain binding C4 zinc finger nuclear receptor DNA-binding transcription factor zinc finger transcription factor gene-specific transcriptional regulator

Process(es)

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PPAR gamma-2 Polyclonal Antibody

PPAR gamma-2 Antibody (PA1-824) in ICC/IF

Product Details

PA1-824

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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