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PPBP Antibody (500-P03GBT-25UG) in ELISA
Sandwich ELISA: To detect Human NAP-2 by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of PPBP Polyclonal Antibody, Biotin (Product # 500-P03GBT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech PPBP Polyclonal Antibody (Product # 500-P03-1MG) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human NAP-2.
Product Details
500-P03GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 7.6 kDa Recombinant Human NAP-2 (CXCL7)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929145
Product Specific Information
AA Sequence of recombinant protein: AELRCMCIKT TSGIHPKNIQ SLEVIGKGTH CNQVEVIATL KDGRKICLDP DAPRIKKIVQ KKLAGDESAD.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human NAP-2 (CXCL7). Anti-Human NAP-2 (CXCL7)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human NAP-2 by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human NAP-2 (CXCL7) (500-P03) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human NAP-2.
Western Blot: To detect Human NAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human NAP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P03GBT-1MG will be provided as 2 x 500 µg
Target Information
Members of the a-chemokine subfamily of inducible, secreted, pro-inflammatory cytokines contain a similar motif, in which the first two cysteine residues are separated by a single residue (Cys-X-Cys), and are also chemotactic for neutrophils. The platelet basic protein (PBP), a member of the a-chemokine family, resides in the a-granules of platelets and is released upon their activation. Proteolytic cleavage of the amino terminus of PBP leads to the generation of several peptides, which include mature PBP, connective tissue-activating peptide III (CTAP III, also designated low affinity platelet factor IV (LA-PF4)), b-thromboglobulin (b-TG), and neutrophil-activating peptide 2 (NAP-2). PBP and its N-truncated derivatives mediate inflammation and wound healing. Specifically, NAP-2 activates chemotaxis and degranulation in neutrophils during inflammation. The gene encoding human PBP maps to chromosome 4q12-q13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: beta-thromboglobulin; C-X-C motif chemokine 7; chemokine (C-X-C motif) ligand 7; connective tissue-activating peptide III; CXC chemokine ligand 7; LDGF; Leukocyte-derived growth factor; low-affinity platelet factor IV; Macrophage-derived growth factor; MDGF; neutrophil-activating peptide 2; neutrophil-activating peptide-2; PBP; Platelet basic protein; pro-platelet basic protein (chemokine (C-X-C motif) ligand 7); small inducible cytokine B7; small inducible cytokine subfamily B, member 7; Small-inducible cytokine B7; thrombocidin 1; thrombocidin 2; thromboglobulin, beta-1
Gene Aliases: B-TG1; Beta-TG; CTAP-III; CTAP3; CTAPIII; CXCL7; LA-PF4; LDGF; MDGF; NAP-2; PBP; PPBP; SCYB7; TC1; TC2; TGB; TGB1; THBGB; THBGB1
UniProt ID: (Human) P02775
Entrez Gene ID: (Human) 5473
leukocyte migration involved in inflammatory response
platelet degranulation
positive regulation of leukocyte chemotaxis
inflammatory response
immune response
G-protein coupled receptor signaling pathway
glucose transport
neutrophil chemotaxis
response to lipopolysaccharide
regulation of cell proliferation
defense response to bacterium
neutrophil degranulation
positive regulation of cell division
chemokine-mediated signaling pathway
glucose transmembrane transport
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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