Proteintech

PPP2CA Polyclonal Antibody

PPP2CA Antibody in Immunocytochemistry (ICC/IF)

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PPP2CA Antibody (13482-1-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA) fixed MCF-7 cells using 13482-1-AP (PPP2CA antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PPP2CA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PPP2CA Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13482-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 24 publications 24 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 4 publications 4 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PPP2CA Fusion Protein Ag4294 (1-309 aa encoded by BC031696)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MDEKVFTKE LDQWIEQLNE CKQLSESQVK SLCEKAKEIL TKESNVQEVR CPVTVCGDVH GQFHDLMELF RIGGKSPDTN YLFMGDYVDR GYYSVETVTL LVALKVRYRE RITILRGNHE SRQITQVYGF YDECLRKYGN ANVWKYFTDL FDYLPLTALV DGQIFCLHGG LSPSIDTLDH IRALDRLQEV PHEGPMCDLL WSDPDDRGGW GISPRGAGYT FGQDISETFN HANGLTLVSR AHQLVMEGYN WCHDRNVVTI FSAPNYCYRC GNQAAIMELD DTLKYSFLQF DPAPRRGEPH VTRRTPDYFL (1-309 aa encoded by BC031696)

Target Information

In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. The PP2A family comprises subfamily members PP2A alpha and PP2A beta. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Berberine stimulates lysosomal AMPK independent of PEN2 and maintains cellular AMPK activity through inhibiting the dephosphorylation regulator UHRF1.

Frontiers in pharmacology

Ren G,Ding YW,Wang LL,Jiang JD

Sun May 07 00:00:00 EDT 2023

Histone phosphorylation integrates the hepatic glucagon-PKA-CREB gluconeogenesis program in response to fasting.

Molecular cell

Zhao Y,Li S,Chen Y,Wang Y,Wei Y,Zhou T,Zhang Y,Yang Y,Chen L,Liu Y,Hu C,Zhou B,Ding Q

Thu Apr 06 00:00:00 EDT 2023

Melatonin increases susceptibility to atrial fibrillation in obesity via Akt signaling impairment in response to lipid overload.

Journal of pineal research

Qin X,Fu Y,Fan J,Liu B,Liu P,Zhang Y,Jiang T,Zheng Q

Sat Apr 01 00:00:00 EDT 2023

Berberine reduces hepatic ceramide levels to improve insulin resistance in HFD-fed mice by inhibiting HIF-2α.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Xia QS,Wu F,Wu WB,Dong H,Huang ZY,Xu L,Lu FE,Gong J

Wed Jun 01 00:00:00 EDT 2022

PP2A-dependent TFEB activation is blocked by PIKfyve-induced mTORC1 activity.

Molecular biology of the cell

Hasegawa J,Tokuda E,Yao Y,Sasaki T,Inoki K,Weisman LS

Tue Mar 01 00:00:00 EST 2022

Nuclear UHRF1 is a gate-keeper of cellular AMPK activity and function.

Cell research

Xu X,Ding G,Liu C,Ding Y,Chen X,Huang X,Zhang CS,Lu S,Zhang Y,Huang Y,Chen Z,Wei W,Liao L,Lin SH,Li J,Liu W,Li J,Lin SC,Ma X,Wong J

Sat Jan 01 00:00:00 EST 2022

Nuclear UHRF1 is a gate-keeper of cellular AMPK activity and function.

Cell research

Xu X,Ding G,Liu C,Ding Y,Chen X,Huang X,Zhang CS,Lu S,Zhang Y,Huang Y,Chen Z,Wei W,Liao L,Lin SH,Li J,Liu W,Li J,Lin SC,Ma X,Wong J

Sat Jan 01 00:00:00 EST 2022

Combination of berberine and low glucose inhibits gastric cancer through the PP2A/GSK3β/MCL-1 signaling pathway.

European journal of pharmacology

Peng Z,Wangmu T,Li L,Han G,Huang D,Yi P

Thu May 05 00:00:00 EDT 2022

Disruption of nuclear envelope integrity as a possible initiating event in tauopathies.

Cell reports

Prissette M,Fury W,Koss M,Racioppi C,Fedorova D,Dragileva E,Clarke G,Pohl T,Dugan J,Ahrens D,Chiu J,Hunt C,Siao CJ,Young T,Bhowmick A,Rogulin V,Desclaux M,Hayden EY,Podgorski M,Gao M,Macdonald LE,Frendewey D,Yancopoulos GD,Zambrowicz B

Tue Aug 23 00:00:00 EDT 2022

WSB1 regulates c-Myc expression through β-catenin signaling and forms a feedforward circuit.

Acta pharmaceutica Sinica. B

Gao X,You J,Gong Y,Yuan M,Zhu H,Fang L,Zhu H,Ying M,He Q,Yang B,Cao J

Tue Mar 01 00:00:00 EST 2022

Sustained IKKβ phosphorylation and NF-κB activation by superoxide-induced peroxynitrite-mediated nitrotyrosine modification of B56γ3 and PP2A inactivation.

Redox biology

Yee YH,Chong SJF,Kong LR,Goh BC,Pervaiz S

Sat May 01 00:00:00 EDT 2021

Programmed cell death 10 promotes metastasis and epithelial-mesenchymal transition of hepatocellular carcinoma via PP2Ac-mediated YAP activation.

Cell death & disease

Sun B,Zhong FJ,Xu C,Li YM,Zhao YR,Cao MM,Yang LY

Tue Sep 14 00:00:00 EDT 2021

Programmed cell death 10 promotes metastasis and epithelial-mesenchymal transition of hepatocellular carcinoma via PP2Ac-mediated YAP activation.

Cell death & disease

Sun B,Zhong FJ,Xu C,Li YM,Zhao YR,Cao MM,Yang LY

Tue Sep 14 00:00:00 EDT 2021

Programmed cell death 10 promotes metastasis and epithelial-mesenchymal transition of hepatocellular carcinoma via PP2Ac-mediated YAP activation.

Cell death & disease

Sun B,Zhong FJ,Xu C,Li YM,Zhao YR,Cao MM,Yang LY

Tue Sep 14 00:00:00 EDT 2021

Vitamin D Attenuates Alzheimer-like Pathology Induced by Okadaic Acid.

ACS chemical neuroscience

Pan Y,Zhang Y,Liu N,Lu W,Yang J,Li Y,Liu Z,Wei Y,Lou Y,Kong J

Wed Apr 21 00:00:00 EDT 2021

Protein phosphatase 1 catalyzes HBV core protein dephosphorylation and is co-packaged with viral pregenomic RNA into nucleocapsids.

PLoS pathogens

Hu Z,Ban H,Zheng H,Liu M,Chang J,Guo JT

Wed Jul 01 00:00:00 EDT 2020

Mouse 4T1 Breast Cancer Cell-Derived Exosomes Induce Proinflammatory Cytokine Production in Macrophages via miR-183.

Journal of immunology (Baltimore, Md. : 1950)

Guo J,Duan Z,Zhang C,Wang W,He H,Liu Y,Wu P,Wang S,Song M,Chen H,Chen C,Si Q,Xiang R,Luo Y

Sun Nov 15 00:00:00 EST 2020

Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.

PLoS biology

He X,Li M,Yu H,Liu G,Wang N,Yin C,Tu Q,Narla G,Tao Y,Cheng S,Yin H

Tue Dec 01 00:00:00 EST 2020

BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells.

Frontiers in cell and developmental biology

Chen B,Zhao Q,Xu T,Yu L,Zhuo L,Yang Y,Xu Y

Mon Sep 28 00:00:00 EDT 2020

The proteasome activator REGγ accelerates cardiac hypertrophy by declining PP2Acα-SOD2 pathway.

Cell death and differentiation

Xie Y,Gao Y,Gao R,Yang W,Dong Z,Moses RE,Sun A,Li X,Ge J

Thu Oct 01 00:00:00 EDT 2020

Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.

Science (New York, N.Y.)

Zheng H,Qi Y,Hu S,Cao X,Xu C,Yin Z,Chen X,Li Y,Liu W,Li J,Wang J,Wei G,Liang K,Chen FX,Xu Y

Fri Nov 27 00:00:00 EST 2020

Role of type 2A phosphatase regulatory subunit B56α in regulating cardiac responses to β-adrenergic stimulation in vivo.

Cardiovascular research

Puhl SL,Weeks KL,Güran A,Ranieri A,Boknik P,Kirchhefer U,Müller FU,Avkiran M

Fri Mar 01 00:00:00 EST 2019

Role of type 2A phosphatase regulatory subunit B56α in regulating cardiac responses to β-adrenergic stimulation in vivo.

Cardiovascular research

Puhl SL,Weeks KL,Güran A,Ranieri A,Boknik P,Kirchhefer U,Müller FU,Avkiran M

Fri Mar 01 00:00:00 EST 2019

KIAA1199 promotes metastasis of colorectal cancer cells via microtubule destabilization regulated by a PP2A/stathmin pathway.

Oncogene

Zhao L,Zhang D,Shen Q,Jin M,Lin Z,Ma H,Huang S,Zhou P,Wu G,Zhang T

Fri Feb 01 00:00:00 EST 2019

KIAA1199 promotes metastasis of colorectal cancer cells via microtubule destabilization regulated by a PP2A/stathmin pathway.

Oncogene

Zhao L,Zhang D,Shen Q,Jin M,Lin Z,Ma H,Huang S,Zhou P,Wu G,Zhang T

Fri Feb 01 00:00:00 EST 2019

PP2Ac Modulates AMPK-Mediated Induction of Autophagy in Mycobacterium bovis-Infected Macrophages.

International journal of molecular sciences

Hussain T,Zhao D,Shah SZA,Sabir N,Wang J,Liao Y,Song Y,Hussain Mangi M,Yao J,Dong H,Yang L,Zhou X

Fri Nov 29 00:00:00 EST 2019

MFHAS1 suppresses TLR4 signaling pathway via induction of PP2A C subunit cytoplasm translocation and inhibition of c-Jun dephosphorylation at Thr239.

Molecular immunology

Shi Q,Xiong B,Zhong J,Wang H,Ma D,Miao C

Tue Aug 01 00:00:00 EDT 2017

MFHAS1 suppresses TLR4 signaling pathway via induction of PP2A C subunit cytoplasm translocation and inhibition of c-Jun dephosphorylation at Thr239.

Molecular immunology

Shi Q,Xiong B,Zhong J,Wang H,Ma D,Miao C

Tue Aug 01 00:00:00 EDT 2017

Glucocorticoid Receptor-mediated transactivation is hampered by Striatin-3, a novel interaction partner of the receptor.

Scientific reports

Petta I,Bougarne N,Vandewalle J,Dejager L,Vandevyver S,Ballegeer M,Desmet S,Thommis J,De Cauwer L,Lievens S,Libert C,Tavernier J,De Bosscher K

Mon Aug 21 00:00:00 EDT 2017

Glucocorticoid Receptor-mediated transactivation is hampered by Striatin-3, a novel interaction partner of the receptor.

Scientific reports

Petta I,Bougarne N,Vandewalle J,Dejager L,Vandevyver S,Ballegeer M,Desmet S,Thommis J,De Cauwer L,Lievens S,Libert C,Tavernier J,De Bosscher K

Mon Aug 21 00:00:00 EDT 2017

Glucocorticoid Receptor-mediated transactivation is hampered by Striatin-3, a novel interaction partner of the receptor.

Scientific reports

Petta I,Bougarne N,Vandewalle J,Dejager L,Vandevyver S,Ballegeer M,Desmet S,Thommis J,De Cauwer L,Lievens S,Libert C,Tavernier J,De Bosscher K

Mon Aug 21 00:00:00 EDT 2017

Glucocorticoid Receptor-mediated transactivation is hampered by Striatin-3, a novel interaction partner of the receptor.

Scientific reports

Petta I,Bougarne N,Vandewalle J,Dejager L,Vandevyver S,Ballegeer M,Desmet S,Thommis J,De Cauwer L,Lievens S,Libert C,Tavernier J,De Bosscher K

Mon Aug 21 00:00:00 EDT 2017

Post-transcriptional modulation of protein phosphatase PPP2CA and tumor suppressor PTEN by endogenous siRNA cleaved from hairpin within PTEN mRNA 3'UTR in human liver cells.

Acta pharmacologica Sinica

Gao YE,Wang Y,Chen FQ,Feng JY,Yang G,Feng GX,Yang Z,Ye LH,Zhang XD

Fri Jul 01 00:00:00 EDT 2016

Cardiac function is regulated by B56α-mediated targeting of protein phosphatase 2A (PP2A) to contractile relevant substrates.

The Journal of biological chemistry