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PPP3CA Polyclonal Antibody

PPP3CA Antibody in Immunocytochemistry (ICC/IF)

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PPP3CA Antibody (13422-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed SH-SY5Y cells using 13422-1-AP (PPP3CA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PPP3CA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PPP3CA Antibody in Flow Cytometry (Flow)

PPP3CA Antibody in Immunoprecipitation (IP)

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Product Details

13422-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:8,000

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 3 publications 3 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse

Published species

Chicken, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PPP3CA Fusion Protein Ag4203 (1-302 aa encoded by BC025714)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSEPKAIDP KLSTTDRVVK AVPFPPSHRL TAKEVFDNDG KPRVDILKAH LMKEGRLEES VALRIITEGA SILRQEKNLL DIDAPVTVCG DIHGQFFDLM KLFEVGGSPA NTRYLFLGDY VDRGYFSIEC VLYLWALKIL YPKTLFLLRG NHECRHLTEY FTFKQECKIK YSERVYDACM DAFDCLPLAA LMNQQFLCVH GGLSPEINTL DDIRKLDRFK EPPAYGPMCD ILWSDPLEDF GNEKTQEHFT HNTVRGCSYF YSYPAVCEFL QHNNLLSILR AHEAQDAGYR MYRKSQTTGF PSL (1-302 aa encoded by BC025714)

Target Information

Calcineurin plays an important role in the T-cell receptor-mediated signal transduction pathway. The protein encoded by this gene binds specifically to the activated form of calcineurin and inhibits calcineurin-mediated signal transduction. The encoded protein is found in the nucleus and contains a leucine zipper domain as well as several PEST motifs, sequences which confer targeted degradation to those proteins which contain them. Alternative splicing results in multiple transcript variants encoding two different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Activation of Kir4.1/Kir5.1 contributes to the cyclosporin A-induced stimulation of the renal NaCl cotransporter and hyperkalemic hypertension.

Acta physiologica (Oxford, England)

Gao ZX,Zhou R,Li MY,Li ST,Mao ZH,Shu TT,Liu DW,Liu ZS,Wu P

Thu Jun 01 00:00:00 EDT 2023

A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.

Molecular psychiatry

Qin Y,Du Y,Chen L,Liu Y,Xu W,Liu Y,Li Y,Leng J,Wang Y,Zhang XY,Feng J,Zhang F,Jin L,Qiu Z,Gong X,Wang H

Fri Jul 01 00:00:00 EDT 2022

The Effect of Cyclosporine A on Proteins Controlling Intracellular Calcium Concentration in Breast Cancer Cells.

The Journal of membrane biology

Caner A,Onal MG,Bitgen N,Sezer G

Tue Feb 01 00:00:00 EST 2022

MicroRNA-30 regulates left ventricular hypertrophy in chronic kidney disease.

JCI insight

Bao J,Lu Y,She Q,Dou W,Tang R,Xu X,Zhang M,Zhu L,Zhou Q,Li H,Zhou G,Yang Z,Shi S,Liu Z,Zheng C

Mon May 24 00:00:00 EDT 2021

Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway.

European journal of pharmacology

Zheng J,Tian J,Wang S,Hu P,Wu Q,Shan X,Zhao P,Zhang C,Guo W,Xu M,Chen H,Lu R

Tue Sep 15 00:00:00 EDT 2020

Transcriptional Study Revealed That Boron Supplementation May Alter the Immune-Related Genes Through MAPK Signaling in Ostrich Chick Thymus.

Biological trace element research

Xiao K,Yang K,Wang J,Sun P,Huang H,Khaliq H,Naeem MA,Zhong J,Peng K

Wed May 01 00:00:00 EDT 2019

Transcriptional Study Revealed That Boron Supplementation May Alter the Immune-Related Genes Through MAPK Signaling in Ostrich Chick Thymus.

Biological trace element research

Xiao K,Yang K,Wang J,Sun P,Huang H,Khaliq H,Naeem MA,Zhong J,Peng K

Wed May 01 00:00:00 EDT 2019

Effect of Hypoxia on the Muscle Fiber Switching Signal Pathways CnA/NFATc1 and Myostatin in Mouse Myocytes.

Acta histochemica

Lixin L,Juan W,Yun B,Jingwei L,Xiuju Y,Xiaomao L,Zhiwei Z,Xiaoyan H,Yanjun D,Hongquan L,Haidong W

Mon Jul 01 00:00:00 EDT 2019

Major contribution of the 3/6/7 class of TRPC channels to myocardial ischemia/reperfusion and cellular hypoxia/reoxygenation injuries.

Proceedings of the National Academy of Sciences of the United States of America

He X,Li S,Liu B,Susperreguy S,Formoso K,Yao J,Kang J,Shi A,Birnbaumer L,Liao Y

Tue Jun 06 00:00:00 EDT 2017

Major contribution of the 3/6/7 class of TRPC channels to myocardial ischemia/reperfusion and cellular hypoxia/reoxygenation injuries.

Proceedings of the National Academy of Sciences of the United States of America

He X,Li S,Liu B,Susperreguy S,Formoso K,Yao J,Kang J,Shi A,Birnbaumer L,Liao Y

Tue Jun 06 00:00:00 EDT 2017

Inhibition of Calcineurin A by FK506 Suppresses Seizures and Reduces the Expression of GluN2B in Membrane Fraction.

Neurochemical research

Wen Y,Fu P,Wu K,Si K,Xie Y,Dan W,Zhan Y,Shi Q

Tue Aug 01 00:00:00 EDT 2017

Inhibition of Calcineurin A by FK506 Suppresses Seizures and Reduces the Expression of GluN2B in Membrane Fraction.

Neurochemical research

Wen Y,Fu P,Wu K,Si K,Xie Y,Dan W,Zhan Y,Shi Q

Tue Aug 01 00:00:00 EDT 2017

Inhibition of Calcineurin A by FK506 Suppresses Seizures and Reduces the Expression of GluN2B in Membrane Fraction.

Neurochemical research

Wen Y,Fu P,Wu K,Si K,Xie Y,Dan W,Zhan Y,Shi Q

Tue Aug 01 00:00:00 EDT 2017

Inhibition of Calcineurin A by FK506 Suppresses Seizures and Reduces the Expression of GluN2B in Membrane Fraction.

Neurochemical research

Wen Y,Fu P,Wu K,Si K,Xie Y,Dan W,Zhan Y,Shi Q

Tue Aug 01 00:00:00 EDT 2017

Calcineurin β protects brain after injury by activating the unfolded protein response.

Neurobiology of disease

Chen Y,Holstein DM,Aime S,Bollo M,Lechleiter JD

Sat Oct 01 00:00:00 EDT 2016

Calcineurin β protects brain after injury by activating the unfolded protein response.

Neurobiology of disease

Chen Y,Holstein DM,Aime S,Bollo M,Lechleiter JD

Sat Oct 01 00:00:00 EDT 2016

Calcineurin β protects brain after injury by activating the unfolded protein response.

Neurobiology of disease

Chen Y,Holstein DM,Aime S,Bollo M,Lechleiter JD

Sat Oct 01 00:00:00 EDT 2016

Calcineurin β protects brain after injury by activating the unfolded protein response.

Neurobiology of disease

Chen Y,Holstein DM,Aime S,Bollo M,Lechleiter JD

Sat Oct 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Calcineurin A alpha; CalcineurinA; Calmodulin-dependent calcineurin A subunit alpha isoform; CALNA 1; CAM-PRP catalytic subunit; CCN 1; CNA alpha; PP2B alpha 1; PP2BA alpha; PP2CB; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform; Protein phosphatase 3 catalytic subunit alpha; protein phosphatase 3 catalytic subunit alpha isozyme; protein phosphatase 3, catalytic subunit, alpha isozyme; Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

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Gene Aliases: 2900074D19Rik; CALN; CALNA; CALNA1; CCN1; CN; CNA; CNA1; PPP2B; PPP3CA

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UniProt ID: (Human) Q08209, (Mouse) P63328

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Entrez Gene ID: (Human) 5530, (Mouse) 19055

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Function(s)

protein serine/threonine phosphatase activity calcium ion binding protein binding calmodulin binding drug binding enzyme binding calmodulin-dependent protein phosphatase activity protein heterodimerization activity protein dimerization activity phosphoprotein phosphatase activity calcium-dependent protein serine/threonine phosphatase activity hydrolase activity metal ion binding protein phosphatase

Process(es)

G1/S transition of mitotic cell cycle response to amphetamine protein dephosphorylation protein import into nucleus calcium ion transport Wnt signaling pathway, calcium modulating pathway transition between fast and slow fiber cardiac muscle hypertrophy in response to stress dephosphorylation calcineurin-NFAT signaling cascade multicellular organismal response to stress negative regulation of chromatin binding cellular response to drug Fc-epsilon receptor signaling pathway T cell activation positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin secretion skeletal muscle fiber development negative regulation of dendrite morphogenesis modulation of synaptic transmission positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NFAT protein import into nucleus response to calcium ion excitatory postsynaptic potential cellular response to glucose stimulus positive regulation of cardiac muscle hypertrophy in response to stress calcium-mediated signaling

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PPP3CA Polyclonal Antibody

PPP3CA Antibody (13422-1-AP) in ICC/IF

Product Details

13422-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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