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Invitrogen
PRAS40 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
Product Details
PA5-31178
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 58 and 206 of Human PRAS40
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2548652
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12.
Predicted reactivity: Mouse (94%), Rat (92%), Chimpanzee (99%), Bovine (95%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia. AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Treatment with the insulin-like growth factor-1 (IGF1) can induce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 40 kDa proline-rich AKT substrate; AKT1 substrate 1 (proline rich); AKT1 substrate 1 (proline-rich); AKT1S1; OTTHUMP00000196756; OTTHUMP00000196760; PKB/Akt substrate 40; Proline-rich AKT substrate; Proline-rich AKT1 substrate 1
Gene Aliases: 1110012J22Rik; AI227026; AKT1S1; Lobe; Lobel; Pras; PRAS40
UniProt ID: (Human) Q96B36, (Mouse) Q9D1F4
Entrez Gene ID: (Human) 84335, (Rat) 292887, (Mouse) 67605
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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