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PRAS40 Antibody (MA5-54104) in ICC/IF
Immunocytochemistry analysis of PRAS40 in C2C12 cells. Nuclei were stained blue with DAPI; PRAS40 recombinant monoclonal antibody (Product # MA5-54104) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Product Details
MA5-54104
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
23GB640
Immunogen
A synthetic peptide of human PRAS40 (150-200AA).
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Target Information
The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia. AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Treatment with the insulin-like growth factor-1 (IGF1) can induce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 40 kDa proline-rich AKT substrate; AKT1 substrate 1 (proline rich); AKT1 substrate 1 (proline-rich); AKT1S1; OTTHUMP00000196756; OTTHUMP00000196760; PKB/Akt substrate 40; Proline-rich AKT substrate; Proline-rich AKT1 substrate 1
Gene Aliases: 1110012J22Rik; AI227026; AKT1S1; Lobe; Lobel; Pras; PRAS40
UniProt ID: (Human) Q96B36, (Mouse) Q9D1F4
Entrez Gene ID: (Human) 84335, (Rat) 292887, (Mouse) 67605
Performance Guarantee
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