Invitrogen

PRAS40 Monoclonal Antibody (73P21)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

8 References

View all (32) PRAS40 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

PRAS40 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

PRAS40 Antibody (AHO1031) in ICC/IF

Immunofluorescent analysis of PRAS40 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with PRAS40 Mouse monoclonal Antibody (Product # AHO1031) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at... View More

PRAS40 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

AHO1031

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunocytochemistry (ICC/IF)

1:100-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

73P21

Immunogen

Recombinant full length human PRAS40 expressed in E. coli.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536321

Target Information

The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia. AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Treatment with the insulin-like growth factor-1 (IGF1) can induce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus.

Nature communications

Zhou X,Zhong Y,Molinar-Inglis O,Kunkel MT,Chen M,Sun T,Zhang J,Shyy JY,Trejo J,Newton AC,Zhang J

AHO1031 was used in Immunocytochemistry-immunoflourescence to reveal a mode of regulation of mTORC1 that is distinct from its lysosomal activation, which controls mTORC1 activity in the nuclear compartment.

Mon Nov 30 00:00:00 EST 2020

Combination of heat shock protein 90 and focal adhesion kinase inhibitors synergistically inhibits the growth of non-small cell lung cancer cells.

Oncoscience

Webber PJ,Park C,Qui M,Ramalingam SS,Khuri FR,Fu H,Du Y

Published figure using PRAS40 monoclonal antibody (Product # AHO1031) in Western Blot

Tue Oct 27 00:00:00 EDT 2015

Characterization of the activity of the PI3K/mTOR inhibitor XL765 (SAR245409) in tumor models with diverse genetic alterations affecting the PI3K pathway.

Molecular cancer therapeutics

Yu P,Laird AD,Du X,Wu J,Won KA,Yamaguchi K,Hsu PP,Qian F,Jaeger CT,Zhang W,Buhr CA,Shen P,Abulafia W,Chen J,Young J,Plonowski A,Yakes FM,Chu F,Lee M,Bentzien F,Lam ST,Dale S,Matthews DJ,Lamb P,Foster P

AHO1031 was used in western blot to study the anti-tumor effects of a pan class I PI3 kinase/mTOR inhibitor in cells with a genetically diverse range of PI3 kinase pathway modifications.

Thu May 01 00:00:00 EDT 2014

Effects of adding exercise to a 16-week very low-calorie diet in obese, insulin-dependent type 2 diabetes mellitus patients.

The Journal of clinical endocrinology and metabolism

Snel M,Gastaldelli A,Ouwens DM,Hesselink MK,Schaart G,Buzzigoli E,Frölich M,Romijn JA,Pijl H,Meinders AE,Jazet IM

Sun Jul 01 00:00:00 EDT 2012

Phosphorylation of PRAS40 on Thr246 by PKB/AKT facilitates efficient phosphorylation of Ser183 by mTORC1.

Cellular signalling

Nascimento EB,Snel M,Guigas B,van der Zon GC,Kriek J,Maassen JA,Jazet IM,Diamant M,Ouwens DM

Tue Jun 01 00:00:00 EDT 2010

Phosphorylation of PRAS40 on Thr246 by PKB/AKT facilitates efficient phosphorylation of Ser183 by mTORC1.

Cellular signalling

Nascimento EB,Snel M,Guigas B,van der Zon GC,Kriek J,Maassen JA,Jazet IM,Diamant M,Ouwens DM

Tue Jun 01 00:00:00 EDT 2010

Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40.

Nature cell biology

Vander Haar E,Lee SI,Bandhakavi S,Griffin TJ,Kim DH

Thu Mar 01 00:00:00 EST 2007

Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40.

Nature cell biology

Vander Haar E,Lee SI,Bandhakavi S,Griffin TJ,Kim DH

Thu Mar 01 00:00:00 EST 2007

Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40.

Nature cell biology

Vander Haar E,Lee SI,Bandhakavi S,Griffin TJ,Kim DH

Thu Mar 01 00:00:00 EST 2007

Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40.

Nature cell biology

Vander Haar E,Lee SI,Bandhakavi S,Griffin TJ,Kim DH

Thu Mar 01 00:00:00 EST 2007

The proline-rich Akt substrate of 40 kDa (PRAS40) is a physiological substrate of mammalian target of rapamycin complex 1.

The Journal of biological chemistry

Oshiro N,Takahashi R,Yoshino K,Tanimura K,Nakashima A,Eguchi S,Miyamoto T,Hara K,Takehana K,Avruch J,Kikkawa U,Yonezawa K

AHO1031 was used in western blot to show that proline-rich Akt substrate of 40 kilodaltons is a raptor-binding protein phosphorylated directly by mammalian target of rapamycin complex 1

Fri Jul 13 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: 40 kDa proline-rich AKT substrate; AKT1 substrate 1 (proline rich); AKT1 substrate 1 (proline-rich); AKT1S1; OTTHUMP00000196756; OTTHUMP00000196760; Proline-rich AKT1 substrate 1

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Gene Aliases: AKT1S1; Lobe; PRAS40

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UniProt ID: (Human) Q96B36

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Entrez Gene ID: (Human) 84335

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Function(s)

protein binding

Process(es)

negative regulation of protein kinase activity negative regulation of TOR signaling regulation of neuron apoptotic process negative regulation of cell size neurotrophin TRK receptor signaling pathway regulation of cellular response to heat

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PRAS40 Monoclonal Antibody (73P21)

PRAS40 Antibody (AHO1031) in ICC/IF

Product Details

AHO1031

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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